This article has Open Peer Review reports available.
The PRICE study (Protection Rest Ice Compression Elevation): design of a randomised controlled trial comparing standard versus cryokinetic ice applications in the management of acute ankle sprain [ISRCTN13903946]
© Bleakley et al; licensee BioMed Central Ltd. 2007
Received: 04 October 2007
Accepted: 19 December 2007
Published: 19 December 2007
Cryotherapy (the application of ice for therapeutic purposes) is one of the most common treatment modalities employed in the immediate management of acute soft tissue injury. Despite its widespread clinical use, the precise physiological responses to therapeutic cooling have not been fully elucidated, and effective evidence-based treatment protocols are yet to be established. Intermittent ice applications are thought to exert a significant analgesic effect. This could facilitate earlier therapeutic exercise after injury, potentially allowing for a quicker return to activity. The primary aim of the forthcoming study is therefore to examine the safety and effectiveness of combining intermittent ice applications with periods of therapeutic exercise in the first week after an acute ankle sprain.
The study is a randomised controlled trial. 120 subjects with an acute grade I or grade II ankle sprain will be recruited from Accident & Emergency and a University based Sports Injury Clinic. Subjects will be randomised under strict double-blind conditions to either a standard cryotherapy (intermittent ice applications with compression) or cryokinetic treatment group (intermittent ice applications with compression and therapeutic exercise). After the first week, treatment will be standardised across groups. Assessor blinding will be maintained throughout the trial. Primary outcome will be function, assessed using the Lower Extremity Functional Scale (LEFS). Additional outcomes will include pain (10 cm Visual Analogue Scale), swelling (modified figure-of-eight method) and activity levels (activ PAL™ physical activity monitor, PAL Technologies, Glasgow, UK). Diagnostic Ultrasound (Episcan-1-200 high frequency ultrasound scanning system, Longport International Ltd, PA) will also be used to assess the degree of soft tissue injury. After baseline assessment subjects will be followed up at 1, 2, 3 & 4 weeks post injury. All data will be analysed using repeated measures analysis of co-variance (ANCOVA).
This paper describes the rationale and design of a randomised controlled trial which will examine the effectiveness of two different cryotherapy protocols in the early management of acute ankle sprain.
Ankle injuries represent one of the most commonly occurring musculoskeletal complaints. The vast majority of such injuries occur as a result of inversion trauma with the foot in some degree of plantar flexion and involve damage to the lateral structures of the ankle. Sprains of the lateral ankle ligaments are associated with significant costs  and account for an estimated 302,000 annual admissions to Accident & Emergency (A&E) Departments in the UK .
In addition to the immediate onset of pain, swelling and loss of joint motion, it has been reported that in 15 – 73% of cases, chronic ankle instability (CAI) with recurrent sprains and residual sensations of giving way may occur following lateral ankle sprain [3, 4]. However, the precise etiology of CAI is unclear and as a consequence the optimal intervention for the management of acute ankle sprain is controversial. While a significant body of evidence supports the use of early functional treatment [5–7], there is little high quality research evidence to suggest which interventions best augment this treatment approach. Clinicians therefore continue to treat such injuries pragmatically, with current recommendations ranging from no intervention to physiotherapy referral, prophylactic bracing, or cast immobilisation [8–11].
Cryotherapy (the application of ice for therapeutic purposes) is a common treatment modality employed in the management of acute soft tissue injuries. Despite its widespread clinical use, the precise physiological responses to ice application have not been fully elucidated. Moreover, the rationale for its use at different stages of recovery is quite distinct. In the acute inflammatory phase after soft tissue injury, cryotherapy is thought to decrease oedema formation via induced vasoconstriction, and reduce secondary hypoxic damage by lowering the metabolic demand of injured tissues [12, 13]. Cooling skin surface temperature to below approximately 15°C is also thought to exert a localised analgesic effect by inhibiting nerve conduction velocity [14, 15]. Short periods of ice application have been used during the later, sub-acute phase of inflammation to produce a similar analgesic effect, thus facilitating earlier and more aggressive therapeutic exercise after muscle injury [16, 17]. This combined use of cryotherapy and exercise has previously been termed cryokinetics . Recent evidence has suggested that the addition of exercise to ice application is more effective than ice application alone after various soft tissue injuries, including acute ankle sprain . However, by reducing the conduction velocity of other, non-nociceptive fibres, cold application may also have a number of deleterious effects, including reduced muscle torque . This is of particular relevance if ice is to be applied in combination with therapeutic exercise in the early stages after an acute soft tissue injury. Such effects could lead to the development of altered neuromuscular control patterns and potentially, to an increased risk of re-injury. Conversely, other evidence has shown that ice application does not negatively affect myotatic reflex activity , joint position sense , plantar flexion torque  or more functional measures of agility [24, 25]. Such conflicting findings may relate to the marked variation in cryotherapy protocols described in the literature, particularly in relation to the site, mode and duration of ice application. These factors, in addition to the level of subcutaneous fat, dictate the degree of superficial and deep tissue cooling, and therefore have a direct effect on the subsequent physiological response to cryotherapy .
Evidence from a large-scale systematic review suggested that intermittent ice applications of 10 minutes are most effective at reducing tissue temperature in both injured animal and healthy human models . Such ice applications have been shown to reduce skin temperature to 5°C immediately after treatment . A recent study by our research group also found that intermittent ice applications are more effective than continuous ice at reducing pain on activity after ankle sprain .
Given these findings, it seems a logical progression to examine if the analgesic effects of intermittent ice application can facilitate earlier therapeutic exercise, and subsequently improve clinical outcome following acute ankle sprain. The safety and effectiveness of incorporating therapeutic exercise with periods of intermittent ice application has not previously been examined in patients with acute soft tissue injury. The primary aim of the forthcoming trial is therefore to compare the effectiveness of standard intermittent versus cryokinetic ice applications in the management of acute grade I and grade II ankle sprains. In this manner we hope to contribute further to the existing evidence base in the area of acute soft tissue injury management.
All patients between 16–65 years with an acute lateral ankle sprain (less than 1 week since injury) will be considered for participation in the study.
Identification of potential subjects
Following initial assessment by an attending clinician (Emergency Nurse Practitioner, Physiotherapist or Doctor) patients with a suspected ankle sprain will be approached by a member of the research team and given a brief verbal explanation of the study. They will then be provided with a detailed study information sheet to consider while awaiting routine x-ray. This will allow for a standard 'cooling off' period of at least 1 hour.
Patients with an acute grade I or grade II sprain will be included in the trial on the basis of a standard physical examination of the acutely injured ankle. As part of this assessment, the following demographic information will be recorded: date of birth, height, mass, gender, limb dominance and occupation. Activity level prior to injury will also be recorded and used to group patients according to whether they are from an athletic (defined as participating in high-intensity exercise for more than 90 minutes at least 3 times per week) or non-athletic population.
Patients will be excluded if any of the following criteria are present: Complete (Grade III) ankle ligament rupture (mechanical instability diagnosed by a positive anterior drawer or inversion stress test); Bony ankle injury (indicated by Ottawa ankle rules  or plain x-ray); Multiple injuries (e.g. other joint injury or fracture); Ankle sprain more than 1 week since injury; Any contraindication to cryotherapy including cryoglobinaemia, peripheral vascular disease or Raynaud's syndrome. Subjects will also be excluded if they are non-English speaking, have any condition which will affect understanding and communication, are under the influence of drugs/alcohol, or if there is no sufficient address given for follow-up.
Baseline outcome measurements
Baseline recording of all outcome measures shall be carried out by the same researcher prior to randomisation.
Primary outcome measure
i) Subjective ankle function, assessed using the Lower Extremity Functional Scale (LEFS) . The LEFS is an 80 point scale which has been shown to have excellent test-retest reliability (intraclass correlation coefficient [ICC] = 0.94, 95% confidence interval [CI] lower limit = 0.89). The scale has a potential error of ± 5.3 points (90% CI), with a minimal detectable change (MDC) and minimal clinically important difference (MICD) of 9 points (both 90% CI).
Secondary outcome measures
ii) Pain assessed using a 10 cm visual analogue scale, marked "no pain" at one end and "worst pain imaginable" at the other. This form of assessment is considered most appropriate because of its high level of repeatability when used serially on the same patient .
iii) Swelling assessed using a modified version of the figure-of-eight method . High intra and inter-rater reliability has been reported using this technique (ICC = 0.99, 95% CI lower limit = 0.98), MDC = 9.6 mm (95% CI). To determine the degree of swelling, the mean value (of 2 measures) will be subtracted from the mean value of the uninjured ankle.
iv) Physical activity levels assessed using the activ PAL™ professional physical activity logger (PAL technologies, Glasgow, UK). Reported ICCs for inter-device reliability range from 0.79–0.97 (CIs not stated) . The activ PAL™ unit (5 cm × 3 cm) will be worn on the thigh of the injured leg, for one week post injury. Time spent sitting, standing and walking under free living conditions will be compared between groups.
Tertiary outcome measure
A statistician who will have no contact with the day to day running of the trial shall carry out all aspects of preparation for group randomisation. 184 randomisation envelopes will be produced in the following manner: 92 cards will be printed with Group I (standard ice application). 92 cards will be printed with Group II (cryokinetic ice application). Each card will be placed inside an opaque envelope with carbon paper on top. A randomisation sequence will then be generated using computer software. Stratified randomisation will be employed according to whether subjects are from an athletic (A) or non-athletic population (N). Separate block randomisation sequences will be produced for each stratum using an allocation ratio of 1:1 and a block size of 4 to ensure comparison groups are of approximate size. Envelopes for each stratum will be labeled sequentially (1A-92A & 1N-92N). Envelopes will then be sealed and signed across the seal. Following baseline assessment, these instructions, in sequentially numbered opaque sealed envelopes, will be used to assign sequentially enrolled subjects to one of the two treatment groups. Subjects shall therefore be randomised to treatment groups under strictly double-blind conditions. Before opening the envelope, the Research Physiotherapist will write the subjects unique identification number (stratification number & date of birth) on the outside of the envelope, transferring this information to the instruction card. At the end of the trial this will allow the Principle Investigator to check each card against the original randomisation lists and verify that all subjects received the treatment to which they were assigned.
All subjects will receive an initial treatment, administered by the same Research Physiotherapist, in A&E or the University Sports Injury Clinic. Standard treatment (Group I) will consist of intermittent ice and compression only. Subjects will receive a 10 minute ice application. The ice pack will then be removed for 10 minutes before a further 10 minute ice application. This will then be followed by a further 10 minutes of rest (10 minutes ice/10 minutes rest/10 minutes ice/10 minutes rest). Cryokinetic treatment (Group II) will consist of intermittent ice and compression with therapeutic exercise. Subjects will receive a 10 minute ice application. The ice pack will then be removed and the subject will perform 10 minutes of therapeutic exercise. This will be followed by another 10 minute ice application and a further 10 minutes of therapeutic exercise (10 minutes ice/10 minutes exercise/10 minutes ice/10 minutes exercise). Subjects will be responsible for ice pack preparation and self administering subsequent treatments at home (3 times per day for the first week after injury). Compliance with treatment and analgesic consumption will be monitored by the use of a treatment diary. Standard advice regarding general mobilisation exercises and weight bearing will also be given to both groups according to routine A&E practice.
Therapeutic exercises for treatment group II
Static ev/in/pf/df (with 10 second hold)
5 of each
Heel slides (with lower limb triple extension)
Static calf stretch (with 30 second hold)
Follow-up procedure & blinding
Blinding of each researcher during trial.
Recruitment, assessment, randomisation and treatment
All baseline and follow-up recording of function, pain, swelling and activity levels (outcome measures i, ii, iii, iv)
Blinded to group allocation
US images (outcome measure v)
Blinded to group allocation
Interpretation of US images (outcome measure v)
Blinded to group allocation
Additional follow up study
In a sub-section to the main trial, longer term function and ankle muscle strength will be examined in a sample of study subjects at 3 months post-injury. While chronic instability and recurrent injuries are a frequent complication following an initial sprain, the precise reasons why such injuries tend to reoccur are unclear at present, and as a consequence rehabilitation may be problematic. It has been suggested that specific evertor muscle strength deficits might be a significant factor in the pathogenesis of CAI [37, 38]. However, other evidence does not support this contention [39, 40]. Previous research has examined conventional eccentric to concentric ratios of individual muscle groups, or concentric evertor to invertor ratios [39–42]. Since dynamic joint stabilization is achieved by co-contraction of the muscles surrounding the joint, it might be more appropriate to divide the eccentric moment of the antagonist by the concentric moment of the agonist . Examination of such dynamic, reciprocal muscle-group ratios represents an alternative approach to the assessment of muscle strength deficits and imbalance following ankle sprain . Here, muscle strength will be assessed using a KinCom 500H isokinetic dynamometer (Chattecx Corp., Hixson, TN). Isokinetic dynamometers provide an accommodating resistance throughout full range of motion, and have been shown to provide a safe and reliable measure of ankle strength . Eccentric and concentric peak torque values for eversion, inversion, plantar flexion and dorsi flexion will be recorded at a velocity of 60° sec-1. Raw data will be normalised for body weight before dynamic ratios are calculated and compared to the un-injured (control) ankle. For example, the eccentric eversion to concentric inversion (eE/cI) ratio for a subject with a body weight (BW) of 88 kg is calculated in the following way: If eE = 81 Newton metres (N.m) (eE/BW = 0.92 N.m/kg) and cI = 92 N.m (cI/BW = 1.15 N.m/Kg), the eE/cI ratio would therefore be 0.92/1.15 = 0.80.
Using the same formula, a sample size calculation has also been carried out to determine the number of subjects required in the 3 month follow up study. This calculation is based on published data  and a clinically meaningful difference between ankle muscle strength ratios of approximately 30%. 30 subjects will be required with the un-injured ankle acting as the control.
Analysis will be on an intention to treat basis. Data will be analysed using SPSS (Windows Version 14.0). Assuming data is from a normal distribution, descriptive statistics will be performed to produce standard deviations (SD), standard errors of the mean (SEM) and 95% confidence intervals (CI). For all outcome measures, a within subject repeated measures analysis of covariance (ANCOVA) will be calculated to determine significant changes over time between groups. Treatment group (two levels: standard and cryokinetic ice application) will be the between subjects factor. Time (four levels: week 1, 2, 3 & 4) will be the within subject factor, with baseline values (week 0) used as the covariate. Where Mauchley's test reveals the assumption of sphericity had been violated the Greenhouse-Geisser epsilon procedure will be carried out to adjust the degrees of freedom accordingly. For all analysis Tukey's test will be used to make post hoc adjustments for multiple comparisons. If any significant interactions are detected between treatment group and time point, a univariate analysis of covariance will be used to indicate the time point at which significant differences are found. If required, analyses will be undertaken using the expectation maximisation algorithm method to impute missing values . A number needed to treat (NNT) analysis will also be carried out. For the 3 month follow-up study, a 2 × 2 ANOVA will be calculated to determine if any significant differences exist between isokinetic ratios of subjects injured and un-injured (control) ankles. The level of significance for all tests will be set at p < 0.05.
Here we have described the rationale and design of a randomised controlled trial comparing standard intermittent versus cryokintic ice applications in the early management of acute grade I and grade II ankle sprain. Although such injuries are often regarded as being fairly innocuous, recurrent sprains and sensations of instability are a frequent sequelae of lateral ankle sprain. Perhaps this is not surprising, given the complexity of the ankle joint and the uncertainty regarding the precise aetiology of chronic ankle instability. However, it may be that current treatment recommendations are insufficient. More intensive initial treatment and advice on potential complications may help to reduce the incidence and associated costs of long-term symptoms after an initial sprain. Intermittent, ten minute periods of ice application and therapeutic exercise in the early stages after injury may represent a simple and cost effective intervention for both athletic and non-athletic populations. However, high quality randomised controlled trials are first required in order to examine the effectiveness of such interventions.
The difficulties of conducting research in an acute setting have previously been highlighted . The process of ensuring all potential patients are assessed for study eligibility will be aided by the researchers being based in A&E on a day to day basis, by working closely with Emergency Nurse Practitioners responsible for triage assessment, and by providing regular trial updates for other relevant clinical staff. Trial profile shall be maintained by placing posters in A&E and by regularly updating media sources including the Hospital and University intranet.
Recruitment will begin in July 2007 and it is anticipated that all data collection will be completed by July 2008. Results of the trial will be disseminated through publication in relevant peer-reviewed journals and conference proceedings.
The authors would like to thank Ms Roisin Devlin, Ms Martina Dunlop, Mr Michael Turley (Emergency Nurse Practitioners) and the staff of the Outpatient Physiotherapy Department at the Royal Victoria Hospital, Belfast for their valued assistance during the trial. We would also like to thank Ms Evie Gardner for her assistance with the treatment allocation and statistical analysis sections of this manuscript. The trial is funded by grants from the Physiotherapy Research foundation (PRF) and the Strategic Priority Fund (SPF) [Department of Employment and Learning, Northern Ireland].
- de Bie RA, de Vet HC, van den Wildenberg FA, Lenssen T, Knipschild PG: The prognosis of ankle sprains. Int J Sports Med. 1997, 18: 285-289. 10.1055/s-2007-972635.View ArticlePubMedGoogle Scholar
- Bridgman SA, Clement D, Downing A, Walley G, Phair I, Maffulli N: Population based epidemiology of ankle sprains attending accident and emergency units in the West Midlands of England, and a survey of UK practice for severe ankle sprains. Emerg Med J. 2003, 20: 508-510. 10.1136/emj.20.6.508.View ArticlePubMedPubMed CentralGoogle Scholar
- Yeung MS, Chan KM, So CH, Yuan WY: An epidemiological survey on ankle sprain. Br J Sports Med. 1994, 28: 112-116.View ArticlePubMedPubMed CentralGoogle Scholar
- Safran MR, Benedetti RS, Bartolozzi AR, Mandelbaum BR: Lateral ankle sprains: a comprehensive review: part 1: etiology, pathoanatomy, histopathogenesis, and diagnosis. Med Sci Sports Exerc. 1999, 31 (7 Suppl): S429-S437.View ArticlePubMedGoogle Scholar
- Kerkhoffs GM, Rowe BH, Assendelft WJ, Kelly KD, Struijs PA, van Dijk CN: Immobilisation for acute ankle sprain. A systematic review. Arch Orthop Trauma Surg. 2001, 121: 462-471. 10.1007/s004020100283.View ArticlePubMedGoogle Scholar
- Kerkhoffs GM, Struijs PA, Marti RK, Blankevoort L, Assendelft WJ, van Dijk CN: Functional treatments for acute ruptures of the lateral ankle ligament: a systematic review. Acta Orthop Scand. 2003, 74: 69-77. 10.1080/00016470310013699.View ArticlePubMedGoogle Scholar
- Jones MH, Amendola AS: Acute treatment of inversion ankle sprains: immobilization versus functional treatment. Clin Orthop Relat Res. 2007, 455: 169-172. 10.1097/BLO.0b013e31802f5468.View ArticlePubMedGoogle Scholar
- Lamb SE, Nakash RA, Withers EJ, Clark M, Marsh JL, Wilson S, Hutton JL, Szczepura A, Dale JR, Cooke MW, Collaborative Ankle Support Trial research team: Clinical and cost effectiveness of mechanical support for severe ankle sprains: design of a randomised controlled trial in the emergency department [ISRCTN 37807450]. BMC Musculoskelet Disord. 2005, 6: 1-10.1186/1471-2474-6-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Cooke MW, Lamb SE, Marsh J, Dale J: A survey of current consultant practice of treatment of severe ankle sprains in emergency departments in the United Kingdom. Emerg Med J. 2003, 20: 505-507. 10.1136/emj.20.6.505.View ArticlePubMedPubMed CentralGoogle Scholar
- Boyce SH, Quigley MA, Campbell S: Management of ankle sprains: a randomised controlled trial of the treatment of inversion injuries using an elastic support bandage or an Aircast ankle brace. Br J Sports Med. 2005, 39: 91-96. 10.1136/bjsm.2003.009233.View ArticlePubMedPubMed CentralGoogle Scholar
- Beynnon BD, Renström PA, Haugh L, Uh BS, Barker H: A prospective, randomized clinical investigation of the treatment of first-time ankle sprains. Am J Sports Med. 2006, 34: 1401-1412. 10.1177/0363546506288676.View ArticlePubMedGoogle Scholar
- Deal DN, Tipton J, Rosencrance E, Curl WW, Smith TL: Ice reduces edema: A study of microvascular permeability in rats. J Bone Joint Surg. 2002, 84-A: 1573-1578.PubMedGoogle Scholar
- Schaser KD, Vollmar B, Menger MD, Schewior L, Kroppenstedt SN, Raschke M, Lubbe AS, Haas NP, Mittlmeier T: In vivo analysis of microcirculation following closed soft-tissue injury. J Orthop Res. 1999, 17: 678-685. 10.1002/jor.1100170509.View ArticlePubMedGoogle Scholar
- Algafly AA, George KP: The effect of cryotherapy on nerve conduction velocity, pain threshold and pain tolerance. Br J Sports Med. 2007, 41: 365-369. 10.1136/bjsm.2006.031237.View ArticlePubMedPubMed CentralGoogle Scholar
- Chesterton LS, Foster NE, Ross L: Skin temperature response to cryotherapy. Arch Phys Med Rehabil. 2002, 83: 543-549. 10.1053/apmr.2002.30926.View ArticlePubMedGoogle Scholar
- Hayden CA: Cryokinetics in an early treatment program. Phys Ther. 1964, 44: 990-993.PubMedGoogle Scholar
- Knight KL, Brucker JB, Stoneman PD, Rubley MD: Muscle injury management with cryotherapy. Athletic Therapy Today. 2000, 5: 26-30.Google Scholar
- Grant AE: Massage with ice (cryokinetics) in the treatment of painful conditions of the musculoskeletal system. Arch Phys Med Rehabil. 1964, 45: 233-238.PubMedGoogle Scholar
- Bleakley C, McDonough S, MacAuley D: The use of ice in the treatment of acute soft-tissue injury: a systematic review of randomized controlled trials. Am J Sports Med. 2004, 32: 251-261. 10.1177/0363546503260757.View ArticlePubMedGoogle Scholar
- Hatzel BM, Kaminski TW: The effects of ice immersion on concentric and eccentric isokinetic muscle performance in the ankle. Isokin and Ex Sci. 2000, 8: 103-107.Google Scholar
- Melnyk M, Faist M, Claes L, Friemert B: Therapeutic cooling: no effect on hamstring reflexes and knee stability. Med Sci Sports Exerc. 2006, 38: 1329-1334. 10.1249/01.mss.0000227635.86285.3b.View ArticlePubMedGoogle Scholar
- Hopper D, Whittington D, Davies J: Does ice immersion influence ankle joint position sense?. Physiother Res Int. 1997, 2: 223-236. 10.1002/pri.108.View ArticlePubMedGoogle Scholar
- Hopkins JT, Stencil R: Ankle cryotherapy facilitates soleus function. J Orthop Sports Phys Ther. 2002, 32: 622-627.View ArticlePubMedGoogle Scholar
- Hart JM, Leonard JL, Ingersoll CD: Single leg standing strategy after knee joint cryotherapy. J Sports Rehab. 2005, 14: 313-320.Google Scholar
- Evans TA, Ingersoll C, Knight KL, Worrell T: Agility Following the Application of Cold Therapy. J Athl Train. 1995, 30 (3): 231-234.PubMedPubMed CentralGoogle Scholar
- Merrick MA, Knight KL, Ingersoll CD, Potteiger JA: The effects of ice and compression wraps on intramuscular temperatures at various depths. J Athl Train. 1993, 28: 236-245.PubMedPubMed CentralGoogle Scholar
- MacAuley D: Ice therapy: how good is the evidence?. Int J Sports Med. 2001, 22: 379-384. 10.1055/s-2001-15656.View ArticleGoogle Scholar
- Ebrall PS, Bales GL, Frost BR: An improved clinical protocol for ankle cryotherapy. J Manual Med. 1992, 6: 161-165.Google Scholar
- Bleakley CM, McDonough SM, MacAuley DC: Cryotherapy for acute ankle sprains: a randomised controlled study of two different icing protocols. Br J Sports Med. 2006, 40: 700-705. 10.1136/bjsm.2006.025932.View ArticlePubMedPubMed CentralGoogle Scholar
- Begg C, Cho M, Eastwood S, Horton R, Moher D, Olkin I, Pitkin R, Rennie D, Schulz KF, Simel D, Stroup DF: Improving the quality of reporting of randomized controlled trials. The CONSORT statement. JAMA. 1996, 276: 637-639. 10.1001/jama.276.8.637.View ArticlePubMedGoogle Scholar
- Steill IG, McKnight RD, Greenberg GH, McDowell I, Nair RC, Wells GA, Johns C, Worthington JR: Implementation of the Ottawa ankle rules. JAMA. 1994, 271: 827-832. 10.1001/jama.271.11.827.View ArticleGoogle Scholar
- Binkley JM, Stratford PW, Lott SA, Riddle DL: The lower extremity functional scale (LEFS) Scale Development, Measurement Properties, and Clinical Application. Phys Ther. 1999, 79: 371-383.PubMedGoogle Scholar
- Katz J, Melzack R: Measurement of pain. Surg Clin North Am. 1999, 79: 231-252. 10.1016/S0039-6109(05)70381-9.View ArticlePubMedGoogle Scholar
- Rohner-Spengler M, Mannion AF, Babst R: Reliability and minimal detectable change for the figure-of-eight-20 method of measurement of ankle edema. J Orthop Sports Phys Ther. 2007, 37: 199-205.View ArticlePubMedGoogle Scholar
- Grant PM, Ryan CG, Tigbe WW, Granat MH: The validation of a novel activity monitor in the measurement of posture and motion during everyday activities. Br J Sports Med. 2006, 40: 992-997. 10.1136/bjsm.2006.030262.View ArticlePubMedPubMed CentralGoogle Scholar
- Knight KL: Cryotherapy in Sports Injury Management. 1995, Champaign IL, Human Kinetics, 220-229.Google Scholar
- Tropp H: Pronator muscle weakness in functional instability of the ankle joint. Int J Sports Med. 1986, 7: 291-294.View ArticlePubMedGoogle Scholar
- Willems T, Witvrouw E, Verstuyft J, Vaes P, De Clercq D: Proprioception and muscle strength in subjects with a history of ankle sprains and chronic instability. J Athl Train. 2002, 37: 487-493.PubMedPubMed CentralGoogle Scholar
- Munn J, Beard DJ, Refshauge KM, Lee RY: Eccentric muscle strength in functional ankle instability. Med Sci Sports Exerc. 2003, 35: 245-250. 10.1249/01.MSS.0000048724.74659.9F.View ArticlePubMedGoogle Scholar
- Ryan L: Mechanical stability, muscle strength and proprioception in the functionally unstable ankle. Aust J Physiother. 1994, 40: 41-47.View ArticlePubMedGoogle Scholar
- Hartsell HD, Spaulding SJ: Eccentric/concentric ratios at selected velocities for the invertor and evertor muscles of the chronically unstable ankle. Br J Sports Med. 1999, 33: 255-258.View ArticlePubMedPubMed CentralGoogle Scholar
- Hubbard TJ, Kramer LC, Denegar CR, Hertel J: Contributing factors to chronic ankle instability. Foot Ankle Int. 2007, 28: 343-354. 10.3113/FAI.2007.0343.View ArticlePubMedGoogle Scholar
- Aagaard P, Simonsen EB, Magnusson SP, Larsson B, Dyhre-Poulsen P: A new concept for isokinetic hamstring: quadriceps muscle strength ratio. Am J Sports Med. 1998, 26: 231-237.PubMedGoogle Scholar
- Kaminski TW, Buckley BD, Powers ME, Hubbard TJ, Ortiz C: Effect of strength and proprioception training on eversion to inversion strength ratios in subjects with unilateral functional ankle instability. Br J Sports Med. 2003, 37: 410-415. 10.1136/bjsm.37.5.410.View ArticlePubMedPubMed CentralGoogle Scholar
- Amaral De Noronha M, Borges NG: Lateral ankle sprain: isokinetic test reliability and comparison between invertors and evertors. Clin Biomech (Bristol, Avon). 2004, 19: 868-871. 10.1016/j.clinbiomech.2004.05.011.View ArticleGoogle Scholar
- Rosner B: Fundamentals of biostatistics. 2000, Pacific Grove CA, Duxbury Press, 331-334. 6Google Scholar
- Chalmers K, Hammond J, Hughes N, Jowett K, Jukes C, O'Connor S, Sajdler C: Isokinetic muscle strength ratios in subjects with chronic ankle instability [Abstract]. J Orthop Sports Phys Ther. 2006, 36: A-18-Google Scholar
- Arbuckle JL: Full information estimation in the presence of incomplete data. Advanced structural equation modelling: issues and techniques. Edited by: Marcoulides GA, Schaumaker RE. 1996, Mahwah NJ, Lawrence Erlbaum Associates, 245-277.Google Scholar
- Cooke M, Wilson S: Obstacles to research in A&E. J Accid Emerg Med. 1997, 14: 269-View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/8/125/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.