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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: In vitro and in vivo tenocyte-protective effectiveness of dehydroepiandrosterone against high glucose-induced oxidative stress

Fig. 3

ROS accumulation . (A) Fluorescence staining showing reactive oxygen species (ROS) accumulation (green) in tenocytes and nuclei (4’ 6-diamidino-2-phenylindole, DAPI) (blue). There was lower ROS accumulation in the low-glucose without dehydroepiandrosterone (LG DHEA–) and with DHEA (LG DHEA+) groups. Increased ROS accumulation observed in the high-glucose (HG) groups, with the HG DHEA + being lower than the HG DHEA– group. (B) Reactive oxygen species (ROS) accumulation was analyzed by fluorescence intensity normalized to cell number. The ROS accumulation in the high-glucose without dehydroepiandrosterone (HG DHEA–) group was greater than that in the low-glucose without DHEA (LG DHEA–) at 48 h. The ROS accumulation in the HG DHEA + group was significantly smaller than that in the HG DHEA– group. *p < 0.05. DAPI, 4’,6-diamidino-2-phenylindole

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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