Skip to main content
Download PDF

Low serum vitamin B12 levels are associated with degenerative rotator cuff tear

BMC Musculoskeletal Disorders volume 22, Article number: 364 (2021) Cite this article

Abstract

Background

Vitamin B12 (Vit B12) deficiency results in elevated homocysteine levels and interference with collagen cross-linking, which may affect tendon integrity. The purpose of this study was to investigate whether serum Vit B12 levels were correlated with degenerative rotator cuff (RC) tear.

Methods

Eighty-seven consecutive patients with or without degenerative RC tear were enrolled as study participants. Possible risk factors (age, sex, medical history, bone mineral density, and serum chemistries including glucose, magnesium, calcium, phosphorus, zinc, homocysteine, Vitamin D, Vit B12, homocysteine, and folate) were assessed. Significant variables were selected based on the results of univariate analyses, and a logistic regression model (backward elimination) was constructed to predict the presence of degenerative RC tear.

Results

In the univariate analysis, the group of patients with degenerative RC tear had a mean concentration of 528.4 pg/mL Vit B12, which was significantly lower than the healthy control group (627.1 pg/mL). Logistic regression analysis using Vit B12 as an independent variable revealed that Vit B12 concentrations were significantly correlated with degenerative RC tear (p = 0.044). However, Vit B12 levels were not associated with tear size.

Conclusion

Low serum levels of Vit B12 were independently related to degenerative RC tear. Further investigations are warranted to determine if Vit B12 supplementation can decrease the risk of this condition.

Peer Review reports

Background

Degenerative rotator cuff (RC) tear typically affects the supraspinatus tendon. This relatively common shoulder lesion can cause significant pain and disability. The number of affected patients is expected to increase as the average life expectancy increases. Approximately 40% of the US population > 60 years of age is affected by this condition annually, and 30,000 to 75,000 RC repairs are performed each year [1]. If untreated, the size of the tear may increase over time. Surgical repair is commonly performed in patients with persistent symptoms [2]. In the USA, the use of the RC repair procedure has increased considerably since 2000, and it is one of the most frequently performed orthopedic surgical procedures in the country [3]. Repair of degenerative RC tears has increased two-fold since 2010 [2, 4].

Study results suggest that the pathogenesis and biochemical changes associated with degenerative RC tear arise from a combination of extrinsic impingement and intrinsic alterations, including increased oxidative stress, neurogenic dysregulation, decreased blood flow, and changes in the extracellular matrix within tendon tissue [5, 6]. Histopathologically, tendon changes include collagen fiber thinning and disorganization, development of granulation tissue, glycosaminoglycan infiltration, fibrocartilaginous metaplasia, calcification, fatty degeneration, and necrosis of the tendon margin with cell apoptosis [7]. These changes are also present in macroscopically intact tendons during the early stages of the degenerative process [7].

Two steps are required to absorb the essential water-soluble vitamin B12 (Vit B12) (cobalamin) from food. First, in the stomach, Vit B12 bound to proteins in food is separated by hydrochloric acid. Vit B12 then combines with gastric intrinsic factor protein and is absorbed by the body. Vit B12 is obtained via ingestion of sources including meat, fish, dairy products, fortified cereals, and supplements [8]. It is crucial for the maintenance of neuronal health and hematopoiesis [9]. Because Vit B12 has antioxidant properties, a deficiency contributes to oxidative stress and the onset of age-related diseases [10]. However, the clinical relationships between Vit B12 deficiency and degenerative diseases remain unclear. Induction of nutrient reallocation to processes necessary for survival can occur during asymptomatic subclinical micronutrient deficiencies [11]. Over the long term, these oxidative stress and other factors contribute to the development of age-related diseases that result from neglect of daily metabolic processes [12].

Studies have not clearly revealed the relationships between Vit B12 levels and degenerative RC tear to the best of our knowledge. The purpose of this study was to evaluate whether serum Vit B12 levels were independently associated with the occurrence of degenerative RC tear.

Methods

Ethics statement

The study protocol (No. 2016–11–009-019) was approved by the institutional review board of this institute. Study procedures were performed following Declaration of Helsinki principles. Written informed consent was obtained from each participant.

Study design and population

In this prospective study, participants were enrolled at a hospital in South Korea between January 2018 and June 2019. Only participants 55–80 years of age were included in the study. Exclusion criteria were patients with a history of malignancy, active infection, auto-immune disease, or previous fracture or surgery of structures in or around the shoulder. No patient had megaloblastic anemia or neuropathy associated with clinically established Vit B12 deficiency.

The study group was divided into the RC tear group and the non-RC tear group. For inclusion in the RC tear group, a patient was required to have a full-thickness RC tear involving the supraspinatus or other RC tendons, or both, and admission to the study institute for arthroscopic RC repair. A non-operative treatment protocol was followed for at least 3 months before surgery. The non-operative treatment protocol included stopping offending activities, physiotherapy (manipulation, stretching, ultrasound, electrical stimulation, and strengthening exercises), corticosteroid injections, anti-inflammatory agents, and extracorporeal shock wave therapy. Only patients with degenerative RC tear were included in the RC group. The condition was confirmed by detailed history taking including the significant trauma history around the shoulder with a physical examination and magnetic resonance imaging (MRI) (Signa Architect 3.0 T; General Electronic Healthcare) to exclude the traumatic RC tear. MRI was used to measure tear size (width and length). During the study period, 75 patients had a diagnosis of full-thickness RC tear and underwent surgical treatment. Fourteen of these patients refused to participate in the study. Thirteen patients were excluded from the study were because the data were incomplete. One patient was excluded because of auto-immune disease (rheumatoid arthritis), malignancy (2 patients), or a history of fracture (3 patients) or surgery in the affected shoulder (2 patients). A total of 40 patients were assigned to the RC tear group.

To be eligible for inclusion in the non-RC tear group, a patient had no RC tear or associated symptoms or clinical signs (e.g., loss of shoulder motion, signs of impingement, tenderness), and visited the institute’s orthopedic clinic. Eligible patients visited the orthopedic clinic during the study period because of minor trauma (dressing superficial lacerations, contusions, and abrasions) of any area except the shoulder region. None previously underwent surgery relating to RC tear. A total of 47 healthy participants who met these criteria and agreed to participate in the study were assigned to the non-RC tear group (Fig. 1).

Fig. 1
figure 1

Flow diagram of results after inclusion and exclusion criteria were applied in this study. *Rotator cuff

Full size image

Variables and data collection

General information collected for each participant included demographic data and medical history. Data on biochemical markers including glucose, magnesium (Mg), calcium (Ca), phosphorus (P), zinc (Zn), homocysteine (HCY), vitamin D (Vit D), folate, and Vit B12 were evaluated. To measure biochemical markers, approximately 10-ml venous blood samples were collected in vacuum-sealed blood tubes in the outpatient or inpatient clinic after an overnight fast. Each sample was centrifuged (3000 rpm, 15 min) and then stored at − 80 °C. Serum electrolyte levels were obtained using a Roche COBAS E701 electrolyte analyzer. Serum Vit B12 was determined via electrochemiluminescence immune assay (ELISA) (Roche Elecsys 2010 analyzer; Switzerland). The remaining components were analyzed using a Siemens Atellica auto-analyzer. All samples were analyzed at the hospital’s laboratory services in the department of clinical pathology at our institution. All staff who performed the analyses were blinded to the study group assignments [13].

Bone mineral density (BMD, g/cm2) was also measured because study findings indicate that BMD is associated with RC tear development [14]. Dual-energy X-ray absorptiometry (Hologic QDR-4500, USA; software version 9.30.044) was used to measure lumbar spine (L1 - L4) and proximal femur (femur neck and total hip) areal BMD.

Arthroscopic examination and RC repair

The same surgeon performed each arthroscopic procedure with the patient in the lateral decubitus position. After the surgeon accessed the subacromial space, partial bursectomy and debridement were used to visualize torn RC tendon margins. When the torn cuff was visible, the RC repairs were performed based on tear size [15].

Sample size calculation and statistical analyses

The results were presented as mean (± standard deviation) values within groups. Variables with P-values < 0.05 were considered statistically significant. The minimum sample size was calculated to ensure sufficient power to analyze the primary outcome variable (RC tear). The parameters included in the sample size estimate were: two-sided t-test, alpha = 0.05, power = 0.80, assumed difference in Vit B12 levels of 21.2% between non-RC tear (744 ± 239 pg/ml) and RC tear (587 ± 215 pg/ml) groups. The values used were based on results of preliminary studies. The calculated minimum sample size was 34 for each group.

To identify correlations between nominal variables and RC tear, Chi-square tests were performed between the RC tear group and non-RC tear group for diabetes mellitus, hypertension, and stroke history. Independent sample t-tests were performed for continuous variables. Continuous variables included glucose, Mg, Ca, P, Zn, Vit D, Vit B12, folate, HCY and BMD.

Logistic regression analysis (backward elimination) was performed to identify independent predictors of RC tear while adjusting for demographic and clinical variables. The variables were retained or deleted based on their statistical contribution. After this process, we were able to find out following 8 variables: Sex, Age, DM, Glucose, P, Vit D, Vit B12, and BMD.

A bivariate analysis was done to compare Vit B12 by RC tear using log-binomial regression to calculate relative risk. We split the Vit B12 according to the quartiles into 4 groups: samples divided by the cut-off point 25th (460.1 pg/mL), 50th (559.0 pg/mL), and 75th (695.5 pg/mL) percentile. Unadjusted and adjusted relative risks and 95% confidence intervals were reported. Adjusted analyses were controlled for eight variables selected by backward elimination regression previously.

Analysis of variance (ANOVA) was used for the analysis of differences in Vit B12 concentrations according to the ranges of retraction (length) and anteroposterior extension (width). These statistical analyses were performed using G*Power software and R version 3.5.2 for Windows.

Results

The mean ages of the patients in the non-RC tear and RC tear groups were 59.3 ± 5.9 years and 61.0 ± 5.3 years, respectively, which was not statistically significant. However, the male had the 1.35 times higher chance of RC tear compared to the female (57.5% vs 42.5%), which was statistically significant (P = 0.017). Among the biochemical markers, the non-RC tear group had the significantly higher serum Vit B12 levels than the RC tear group (528.4 ± 145.7 pg/mL for the RC tear vs 627.1 ± 183.0 pg/mL for the non-RC tear group) (P = 0.007). The mean Vit D level was also lower in the RC tear group as 15.7 ± 7.2 ng/mL compared to the non-RC tear group as 21.6 ± 10.0 ng/mL with the statistical significance (P = 0.002). Next, the mean P level was significantly different between RC tear and non-RC tear group as 3.2 ± 0.6 mg/dL and 3.6 ± 0.7 mg/dL, respectively (P = 0.008). The other parameters showed no significant relationships with RC tear in the univariate analyses. Table 1 presents results for the baseline clinical and biochemical characteristics of each group.

Table 1 Demographic characteristics of all variables, non-RC tear group versus RC tear group
Full size table

After controlling for confounding variables in logistic regression (backward elimination), the following variables were independently associated with RC tear; Sex, DM, Age, Glucose, Vit D, and Vit B12 (Table 2). Sex was more likely (odds ratio [OR] = 7.96; 95% confidence interval [CI] = 1.9–41.57; P = 0.008) to receive a significant variable among RC tear group and non-RC tear group. Age was positively associated with RC tear, after adjustment for other factors (OR = 1.18; 95% CI = 1.05–1.36; P = 0.009). DM was associated with RC tear (OR = 8.74; 95% CI = 1.24–76.79; P = 0.035). Glucose (OR = 0.97; 95% CI = 0.93–0.99; P = 0.031), Vit D (OR = 0.89; 95% CI = 0.82–0.96; P = 0.006) were negatively associated with RC tear. The analysis revealed that after adjustment for other factors, serum Vit B12 level was negatively associated with RC tear (OR = 0.99; 95% CI = 0.99–1; P = 0.044).

Table 2 Multivariate analysis: Logistic regression with backward elimination selection AIC
Full size table

Next, low Vit B12 group had the higher RR for the RC tear in general. In comparison to the ‘Vit B12 < 460.1 pg/mL’ group, the ‘559 - 695.5’ and ‘695.5 -’ group were less likely to occur degenerative RC tear (RR = 0.73, CI = 0.34–1.45 and RR = 0.64, CI = 0.28–1.31). While the 460.1–559.0 group was more likely to occur degenerative RC tear (RR = 0.73, CI = 0.34–1.45) (Table 3).

Table 3 Relative risks of Vit B12 group according to RC tear
Full size table

We also performed an ANOVA to determine whether there was a correlation between RC tear size and Vit B12 level in the RC tear group only. The results indicated that there was no significant difference in Vit B12 level and MRI-measured RC tear size. These results are presented in Supplemental Table 1.

Discussion

This study was designed to examine whether there was an association between serum Vit B12 levels and degenerative RC tear in adults 55 to 80 years of age. Factors found to affect degenerative RC tear include age, diabetes, hyperlipidemia, and Vit D deficiency [16]. Few studies have reported results on the relationship between Vit B12 and RC tear. We measured Vit B12 levels prospectively in RC tear and non-RC tear patients and found that patients with lower Vit B12 levels had greater odds of having a degenerative RC tear.

Hashimoto et al. [17] reported age-related histological changes including collagen fiber thinning, disorientation, and fatty infiltration in the RC tear. Tendon properties and functions are causally related to the architecture and quality of collagen fibers. Collagen is the primary extracellular matrix of RC tendons, and the major focus of tenocyte metabolism is to maintain matrisome integrity by regulating collagen [18].

The pathogenesis of degenerative RC tear remains incompletely understood but can include impaired collagen synthesis, oxidative stress, chronic inflammation, ischemia, and impaired healing [19]. Tendinopathy pathogenesis may include decreased tendon cell collagen synthesis and increased collagen degradation in the tendon matrix [20]. Due to age-dependent reductions in tendon cells and enzymes essential for collagen synthesis, collagen synthesis declines with age. Delayed repair of soft tissues such as tendons is also associated with old age [21, 22]. These changes create a more fragile injury-prone area. Individuals who perform repetitive tasks during daily activities, jobs, or sports are at greater risk of these changes [23].

Some previous studies that reported the roles of Vit B12 in collagen production and cross-linking included consideration of HCY. Vit B12 is an essential HCY degrading remethylation and trans sulfuratin pathways co-enzyme [11]. Therefore, Vit B12 deficiency is a main cause of elevated HCY serum concentrations. Considering hyperhomocysteinemia and collagen, Lee et al. [24] investigated the effect of HCY on the production of matrix metalloproteinase (MMP). They concluded that increased HCY levels are associated with increased MMP production, which is implicated in collagen cross-linking breakdown and related to poor healing of RC tendon tears [25]. Robert et al. [26] found that a high serum HCY concentration may weaken bone by interfering with collagen cross-linking. Our findings also suggested that Vit B12 has a specific role that affects RC tendon structural properties via the pathways mentioned above.

Oxidative stress has an important role in tendinopathy development [27, 28]. Reactive oxygen species (ROS) modulate physiological events (e.g., tenocyte proliferation and differentiation, inflammation, and healing) [29]. Cell injury, senescence, and death can be caused by extensive ROS exposure or insufficient cellular antioxidant capacity, or both [30]. Vit B12 might be implicated in the pathogenesis of tendinopathy via modulation of oxidative stress. Potential Vit B12 antioxidant properties include: (1) direct ROS (e.g., superoxide) scavenging; (2) indirect ROS scavenging stimulation via glutathione preservation; (3) cytokine and growth factor production modulation for protection from oxidative stress induced by the immune response; (4) reduction of homocysteine-induced oxidative stress; and (5) reduction of oxidative stress caused by advanced glycation end products [10]. Tomohiro et al. [31] also found reductions in cellular L-ascorbic acid levels. During Vit B12 deficiency, this potent antioxidant participates in collagen biosynthesis in most mammals. They found that reductions resulted in concurrent disordered biosynthesis of worm collagen [32].

Cytokines regulate host immune responses to inflammation, infection, and trauma. These molecular messengers participate in matrix turnover during tendinopathy, tenocyte activity, and tendon matrix gene expression [33]. Among the various cytokines, up-regulation of pro-inflammatory cytokines is associated with RC tendinopathy in rat and human models [34]. Al-Daghri et al. [35] measured systemic Vit B12 concentrations with pro-inflammatory cytokines and concluded that serum Vit B12 concentrations were associated with pro-inflammatory cytokines. In their in vitro study, Jinous et al. [36] found that low adipocyte Vit B12 levels induced greater gene expression and secretion of pro-inflammatory cytokines. Their results suggest that this change results in adipocyte dysfunction.

Regarding the diagnostic criteria for Vit B12 deficiency, the normal range is classically defined as 200–900 (or 700) pg/mL [13]. Using these criteria, all except two participants had levels within the normal range. Some authors suggest even lower levels (e.g., 100 pg/mL) as the lower limit of the normal range [37]. However, this normal range was defined based on the development of anemia or neurological disorders and treatment using Vit B12 supplementation. Green and Hannibal et al. suggested that subclinical Vit B12 levels are 119–200 pmol/L (161.28–271.07 pg/mL) serum Vit B12 [9, 38]. However, while an individual remains apparently symptom-free, these subclinical levels can induce long-term damage to macromolecules (e.g., nucleic acids, proteins, and lipids) [39]. In this context, Mitsuyama et al. proposed the optimal range of Vit B12 as 500–1300 pg/mL although the classical normal range is much lower [40]. Likewise, other experts suggested that the optimal range for HCY is < 7 μmol/L for good health although the classical standard reference range was 5.0–12 μmol/L and these optimal range had a significant association with a lower likelihood of stroke, atherosclerosis, and improved overall cardiovascular function [41]. Collectively, the classical normal range might not be applicable in this study because the relationships between Vit B12 and other aging-related diseases such as degenerative tendinopathy were not considered.

This study had some limitations. First, we did not examine whether Vit B12 supplementation improved clinical results. Research is needed to investigate the effects of Vit B12 supplementation on healing after an RC tear. Second, as discussed above, Vit B12 deficiency is closely related to higher HCY levels. Still, although it was higher in the non-RC tear group, serum HCY levels were not significantly different between the RC tear and non-RC tear groups (P = 0.774, Table 1). We speculate that the sample size was insufficient to detect a statistically significant difference. Long-term follow-up studies with larger groups of patients and focusing on HCY are needed. Last, we measured Vit B12 only one time. To analyze the long-term effects of low serum Vit B12 levels, serial follow-up measurements of Vit B12 levels should be performed.

Conclusions

In conclusion, few studies about the relationship between Vit B12 and degenerative tendinopathy have been reported to date. Our results suggested that Vit B12 deficiency is an independent risk factor for RC tear. Further investigations are needed to understand relationships between the effects of Vit B12 and its byproducts on the structural properties of the RC tendon.

Availability of data and materials

The datasets generated during and analyzed during the current study are not available due to privacy or ethical restrictions but are available from the corresponding author on reasonable request.

Abbreviations

Vit B12 :

Vitamin B12

RC:

Rotator cuff

MRI:

Magnetic resonance imaging

Mg:

Magnesium

Ca:

Calcium

P:

Phosphorus

Zn:

Zinc

HCY:

Homocysteine

Vit D:

Vitamin D

ELISA:

Electrochemiluminescence immune assay

BMD:

Bone mineral density

ANOVA:

Analysis of variance

OR:

Odds ratio

MMP:

Matrix metalloproteinase

ROS:

Reactive oxygen species

References

  1. Maffulli N, Longo UG, Loppini M, Berton A, Spiezia F, Denaro V. Tissue engineering for rotator cuff repair: an evidence-based systematic review. Stem Cells Int. 2012;2012:418086.

    Article  Google Scholar 

  2. Park JG, Cho NS, Song JH, Baek JH, Jeong HY, Rhee YG. Rotator cuff repair in patients over 75 years of age: clinical outcome and repair integrity. Clin Orthop Surg. 2016;8(4):420–7. https://doi.org/10.4055/cios.2016.8.4.420.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Austin DC, Torchia MT, Lurie JD, Jevsevar DS, Bell JE. Mapping the diffusion of Technology in Orthopaedic Surgery: understanding the spread of arthroscopic rotator cuff repair in the United States. Clin Orthop Relat Res. 2019;477(11):2399–410. https://doi.org/10.1097/CORR.0000000000000860.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Schairer WW, Nwachukwu BU, Fu MC, Warren RF. Risk factors for short-term complications after rotator cuff repair in the United States. Arthroscopy. 2018;34(4):1158–63. https://doi.org/10.1016/j.arthro.2017.10.040.

    Article  PubMed  Google Scholar 

  5. Han SH, Choi W, Song J, Kim J, Lee S, Choi Y, et al. The Implication of Substance P in the Development of Tendinopathy: a Case Control Study. Int J Mol Sci. 2017;18:6.

    Google Scholar 

  6. Garofalo R, Cesari E, Vinci E, Castagna A. Role of metalloproteinases in rotator cuff tear. Sports Med Arthrosc Rev. 2011;19(3):207–12. https://doi.org/10.1097/JSA.0b013e318227b07b.

    Article  PubMed  Google Scholar 

  7. Chillemi C, Petrozza V, Garro L, Sardella B, Diotallevi R, Ferrara A, et al. Rotator cuff re-tear or non-healing: histopathological aspects and predictive factors. Knee Surg Sports Traumatol. 2011;19(9):1588–96. https://doi.org/10.1007/s00167-011-1521-1.

    Article  CAS  Google Scholar 

  8. Langan RC, Goodbred AJ. Vitamin B12 deficiency: recognition and management. Am Fam Physician. 2017;96(6):384–9.

    PubMed  Google Scholar 

  9. Green R, Allen LH, Bjorke-Monsen AL, Brito A, Gueant JL, Miller JW, et al. Vitamin B12 deficiency. Nat Rev Dis Primers. 2017;3(1):17040. https://doi.org/10.1038/nrdp.2017.40.

    Article  PubMed  Google Scholar 

  10. van de Lagemaat EE, de Groot L, van den Heuvel E. Vitamin B12 in Relation to Oxidative Stress: A Systematic Review. Nutrients. 2019;11:2.

    Google Scholar 

  11. Ames BN. Prevention of mutation, cancer, and other age-associated diseases by optimizing micronutrient intake. J Nucleic Acids. 2010;2010:725071.

    Article  Google Scholar 

  12. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018;13:757–72. https://doi.org/10.2147/CIA.S158513.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Hanna S, Lachover L, Rajarethinam RP. Vitamin b(1)(2) deficiency and depression in the elderly: review and case report. Primary Care Companion J Clin Psychiatry. 2009;11(5):269–70. https://doi.org/10.4088/PCC.08l00707.

    Article  PubMed  Google Scholar 

  14. Chen X, Giambini H, Ben-Abraham E, An KN, Nassr A, Zhao C. Effect of bone mineral density on rotator cuff tear: An osteoporotic rabbit model. PLoS One. 2015;10(10):e0139384. https://doi.org/10.1371/journal.pone.0139384.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Lo IK, Burkhart SS. Current concepts in arthroscopic rotator cuff repair. Am J Sports Med. 2003;31(2):308–24.

    Article  Google Scholar 

  16. Zumstein M-A, Lädermann A, Raniga S, Schär M-OJO, Surgery T. Research: The biology of rotator cuff healing. Orthop Traumatol Surg Res. 2017;103(1):S1–S10.

    Article  Google Scholar 

  17. Hashimoto T, Nobuhara K, Hamada TJCO. Research® R: Pathologic evidence of degeneration as a primary cause of rotator cuff tear. Clin Orthop Relat Res. 2003;415:111–20.

    Article  Google Scholar 

  18. Thankam FG, Dilisio MF, Gross RM, Agrawal DK. Collagen I: a kingpin for rotator cuff tendon pathology. Am J Transl Res. 2018;10(11):3291–309.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Reinking M. Tendinopathy in athletes. Phys Ther Sport. 2012;13(1):3–10. https://doi.org/10.1016/j.ptsp.2011.06.004.

    Article  PubMed  Google Scholar 

  20. Riley GP, Harrall RL, Cawston TE, Hazleman BL, Mackie EJ. Tenascin-C and human tendon degeneration. Am J Pathol. 1996;149(3):933.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Kannus P, Paavola M, Józsa L. Aging and degeneration of tendons. Tendon Injuries. 2005;1:25–31. https://doi.org/10.1007/1-84628-050-8_4.

    Article  Google Scholar 

  22. Kannus P, Jozsa LG, Josza L. Human tendons: anatomy, physiology, and pathology: Human Kinetics Publishers; 1997.

    Google Scholar 

  23. Tuite D, Renström P, O’brien MJ. The aging tendon. Scand J Med Sci Sports. 1997;7(2):72–7.

    Article  CAS  Google Scholar 

  24. Lee SJ, Lee YS, Seo KW, Bae JU, Kim GH, Park SY, et al. Homocysteine enhances MMP-9 production in murine macrophages via ERK and Akt signaling pathways. Toxicol Appl Pharmacol. 2012;260(1):89–94.

    Article  CAS  Google Scholar 

  25. Reider B. Big D. Am J Sports Med. 2014;42(1):25–6. https://doi.org/10.1177/0363546513516922.

    Article  PubMed  Google Scholar 

  26. McLean RR, Jacques PF, Selhub J, Tucker KL, Samelson EJ, Broe KE, et al. Homocysteine as a predictive factor for hip fracture in older persons. N Engl J Med. 2004;350(20):2042–9.

    Article  CAS  Google Scholar 

  27. Longo UG, Berton A, Papapietro N, Maffulli N, Denaro V. Epidemiology, genetics and biological factors of rotator cuff tears. Med Sport Sci. 2012;57:1–9. https://doi.org/10.1159/000328868.

    Article  PubMed  Google Scholar 

  28. Kaleagasioglu F, Olcay E. Fluoroquinolone-induced tendinopathy: etiology and preventive measures. Tohoku J Exp Med. 2012;226(4):251–8. https://doi.org/10.1620/tjem.226.251.

    Article  CAS  PubMed  Google Scholar 

  29. Sies H. Role of metabolic H2O2 generation: redox signaling and oxidative stress. J Biol Chem. 2014;289(13):8735–41. https://doi.org/10.1074/jbc.R113.544635.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Zou H, Stoppani E, Volonte D, Galbiati F. Caveolin-1, cellular senescence and age-related diseases. Mech Ageing Dev. 2011;132(11–12):533–42. https://doi.org/10.1016/j.mad.2011.11.001.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Bito T, Misaki T, Yabuta Y, Ishikawa T, Kawano T, Watanabe F. Vitamin B12 deficiency results in severe oxidative stress, leading to memory retention impairment in Caenorhabditis elegans. Redox Biol. 2017;11:21–9. https://doi.org/10.1016/j.redox.2016.10.013.

    Article  CAS  PubMed  Google Scholar 

  32. Du J, Cullen JJ, Buettner GR. Ascorbic acid: chemistry, biology and the treatment of cancer. Biochim Biophys Acta. 2012;1826(2):443–57. https://doi.org/10.1016/j.bbcan.2012.06.003.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Maffulli N, Longo UG, Berton A, Loppini M, Denaro V. Biological factors in the pathogenesis of rotator cuff tears. Sports Med Arthrosc Rev. 2011;19(3):194–201. https://doi.org/10.1097/JSA.0b013e3182250cad.

    Article  PubMed  Google Scholar 

  34. Millar NL, Wei AQ, Molloy TJ, Bonar F, Murrell GA. Cytokines and apoptosis in supraspinatus tendinopathy. J Bone Joint Surg Br Vol. 2009;91(3):417–24.

    Article  CAS  Google Scholar 

  35. Al-Daghri NM, Rahman S, Sabico S, Yakout S, Wani K, Al-Attas OS, et al. Association of Vitamin B12 with Pro-Inflammatory Cytokines and Biochemical Markers Related to Cardiometabolic Risk in Saudi Subjects. Nutrients. 2016;8:9.

    Article  Google Scholar 

  36. Samavat J, Adaikalakoteswari A, Boachie J, Saravanan P. Increased pro-inflammatory cytokine production in vitamin B12 deficient adipocytes. In: Society for Endocrinology BES 2018, vol. 2018; 2018. BioScientifica.

    Google Scholar 

  37. Oh R, Brown DL. Vitamin B12 deficiency. Am Fam Physician. 2003;67(5):979–86.

    PubMed  Google Scholar 

  38. Hannibal L, Lysne V, Bjorke-Monsen AL, Behringer S, Grunert SC, Spiekerkoetter U, et al. Biomarkers and algorithms for the diagnosis of vitamin B12 deficiency. Front Mol Biosci. 2016;3:27.

    Article  Google Scholar 

  39. Ames BN. Low micronutrient intake may accelerate the degenerative diseases of aging through allocation of scarce micronutrients by triage. Proc Natl Acad Sci U S A. 2006;103(47):17589–94. https://doi.org/10.1073/pnas.0608757103.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Mitsuyama Y, Kogoh H. Serum and cerebrospinal fluid vitamin B12 levels in demented patients with CH3-B12 treatment--preliminary study. Japanese J Psychiatry Neurol. 1988;42(1):65–71.

    CAS  Google Scholar 

  41. Iso H, Moriyama Y, Sato S, Kitamura A, Tanigawa T, Yamagishi K, et al. Serum total homocysteine concentrations and risk of stroke and its subtypes in Japanese. Circulation. 2004;109(22):2766–72. https://doi.org/10.1161/01.CIR.0000131942.77635.2D.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

Role of funder (NRF and MSIT): Financial support and administrative support.

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2019R1F1A1061015, 2019R1A2C4070492, 2019R1C1C1004017).

Author information

Authors and Affiliations

  1. Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea

    Jae Hwa Kim, Go-Tak Kim, Siyeoung Yoon, Sang-Cheol Lee & Soonchul Lee

  2. Department of Orthopedic Surgery, Ilsan Paik Hospital, Inje University, Goyang-si, Gyeonggi-do, 10380, Republic of Korea

    Hyun Il Lee

  3. Department of Orthopaedic Surgery, Samsung Medical Center, Sungkyunkwan University, School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul, 06351, Republic of Korea

    Kyung Rae Ko

  4. CHA Graduate School of Medicine, 120 Hyeryong-ro, Pocheon, 11160, Republic of Korea

    Do Kyung Kim

  5. Department of Computer Science, College of IT Engineering, SeMyung University, Semyung-ro, Jecheon-si, Chung-cheong bukdo, 27136, South Korea

    Jaeyeon Shin

  6. Department of Internal Medicine, CHA Bundang Medical Center, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea

    So-young Lee

Authors
  1. Jae Hwa Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Go-Tak Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Siyeoung Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hyun Il Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kyung Rae Ko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sang-Cheol Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Do Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jaeyeon Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. So-young Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Soonchul Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.K.: Project administration; formal analysis; writing original draft. G.K.: Manuscript review and editing. S.Y.: Formal analysis, methodology. H.L.: Formal analysis, methodology. K.K.: Formal analysis. S.L.: Data curation. D.K.: Data curation. J.S.: Data curation. S.-Y.L.: Manuscript review and editing. S.C.L.: Conceptualization; funding acquisition; investigation; manuscript review and editing. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Soonchul Lee.

Ethics declarations

Ethics approval and consent to participate

Institutional review board of CHA medical center approved this study. (No. 2016–11–009-019).

Written informed consent was obtained from each participant.

Consent for publication

Written informed consent was obtained from all study participants for publication.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1: Table S1.

Correlation between serum Vit B12 level and RC tear size measured by MRI.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, J.H., Kim, GT., Yoon, S. et al. Low serum vitamin B12 levels are associated with degenerative rotator cuff tear. BMC Musculoskelet Disord 22, 364 (2021). https://doi.org/10.1186/s12891-021-04231-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12891-021-04231-7

Keywords

  • Vitamin B12
  • Rotator cuff
  • Degenerative
  • Tear

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us