Figure 5

Histological analyses 10 days after in vivo electroporation. Histological examinations were carried out 10 days after electroporation with pCAGGS and pCAGGS-BMP-2 or pCAGGS-BMP-2 and pCAGGS-BMP-7. A section stained with HE from muscle electroporated with pCAGGS and pCAGGS-BMP-2 (A) contained cartilage (arrow). A serial section stained with von Kossa showing partially calcified areas (B, arrows). A HE-stained section of muscle electroporated with pCAGGS-BMP-2 and pCAGGS-BMP-7 showing ectopic bone (C, arrows). The calcified areas that stained with von Kossa (D, arrows) in the pCAGGS-BMP-2 and pCAGGS-BMP-7-transfected muscles were much wider and more strongly stained than in the BMP-2-transfected sections (B). Scale bar: 100 μm.