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Figure 2 | BMC Musculoskeletal Disorders

Figure 2

From: Simultaneous gene transfer of bone morphogenetic protein (BMP) -2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression

Figure 2

Simultaneous gene transfer with BMP-2 and BMP-7 in vitro. ALP activity in C2C12 cells transfected with pCAGGS, pCAGGS and pCAGGS-BMP-2, pCAGGS and pCAGGS-BMP-7, or pCAGGS-BMP-2 and pCAGGS-BMP-7. ALP staining in the C2C12 cells 10 days after the treatment (A). There were many more ALP-positive clusters in the combined (BMP-2 and BMP-7) gene transfer group than in cultures transfected with either gene alone. Quantitative analysis of the ALP activity in C2C12 cells transfected with BMP-2, BMP-7, or the combined BMP-2 and BMP-7 genes (B). A more than five-fold increase in ALP activity was seen after gene transfer with pCAGGS-BMP-2 and pCAGGS-BMP-7 compared with pCAGGS-BMP-7 or pCAGGS-BMP-2 alone, respectively. (*P < 0.05.)

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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