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Table 1 Primer sequences used for relative real-time RT-PCR

From: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

Gene Accession number Forward (5′-3′) Reverse (5′-3′)
bovine (Bos taurus)
Bt_r18S DQ222453.1 ACG GAC AGG ATT GAC AGA TTG CCA GAG TCT CGT TCG TTA TCG
Bt_ACAN NM_173981.2 GGC ATC GTG TTC CAT TAC AG ACT CGT CCT TGT CTC CAT AG
Bt_CD24 NC_007300.4 AGA CTT ACT CAA ATC AAA AAC AGT AGA GAT GTA GAA
Bt_col1 A2 NM_174520.2 GCC TCG CTC ACC AAC TTC AGT AAC CAC TGC TCC ATT CTG
Bt_col2 A1* AY743675.1 CGG GTG AAC GTG GAG AGA CA GTC CAG GGT TGC CAT TGG AG
porcine (Sus scrofa domestica)
Ssd_r18S NR_046261.1 TAG AAG GAA GAG GAA CCA T TAA TGT CCA ACT CAC TGA AG
Ssd_ACAN NM_001164652.1 CAG TAA CTT CGT GCC TAG GGT CCT CTA TCT CCA GTT
Ssd_T (brachyury)* NC_010443.4 TCA CCA ACA AG CTCA ATG CTC TCA CGA TGT GGA TTC
Ssd_col1 A2 AB237775.1 TAT CGG AAT TAA CCA GAC A ACA GGA TTG ACA GAT TGA
  1. *Denotes primer match for both species.
  2. The PCR was run in a two-step protocol; 45 cycles with a 2-step protocol and an annealing temperature at 57°C.