Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

Table 1 Primer sequences used for relative real-time RT-PCR

Gene	Accession number	Forward (5′-3′)	Reverse (5′-3′)
bovine (Bos taurus)
Bt_r18S	DQ222453.1	ACG GAC AGG ATT GAC AGA TTG	CCA GAG TCT CGT TCG TTA TCG
Bt_ACAN	NM_173981.2	GGC ATC GTG TTC CAT TAC AG	ACT CGT CCT TGT CTC CAT AG
Bt_CD24	NC_007300.4	AGA CTT ACT CAA ATC AAA	AAC AGT AGA GAT GTA GAA
Bt_col1 A2	NM_174520.2	GCC TCG CTC ACC AAC TTC	AGT AAC CAC TGC TCC ATT CTG
Bt_col2 A1*	AY743675.1	CGG GTG AAC GTG GAG AGA CA	GTC CAG GGT TGC CAT TGG AG
porcine (Sus scrofa domestica)
Ssd_r18S	NR_046261.1	TAG AAG GAA GAG GAA CCA T	TAA TGT CCA ACT CAC TGA AG
Ssd_ACAN	NM_001164652.1	CAG TAA CTT CGT GCC TAG	GGT CCT CTA TCT CCA GTT
Ssd_T (brachyury)*	NC_010443.4	TCA CCA ACA AG CTCA ATG	CTC TCA CGA TGT GGA TTC
Ssd_col1 A2	AB237775.1	TAT CGG AAT TAA CCA GAC A	ACA GGA TTG ACA GAT TGA

*Denotes primer match for both species.
The PCR was run in a two-step protocol; 45 cycles with a 2-step protocol and an annealing temperature at 57°C.

ISSN: 1471-2474