Figure 6

Relative gene expression of porcine NC in co-culture with bovine NPC in 3D alginate microspheres after 14 days of culture in normoxic (in blue) and hypoxic conditions (in brown). A. Aggrecan (ACAN) expression, B. Collagen type 2 (col2) expression, C. Brachyury (T) expression, D. CD24 expression *indicates p < 0.05. Gene expression is given relative to the pure NC without FCS and in normoxia (NO) at 14 days. CoC = co-culture with NPC, FCS = 7 days pre-culture in medium containing 10% fetal calf serum (mean ± SEM, N = 4 co-culture repeats).