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Quantitative analysis of the reversibility of knee flexion contractures with time: an experimental study using the rat model
© Trudel et al.; licensee BioMed Central Ltd. 2014
Received: 26 May 2014
Accepted: 29 September 2014
Published: 7 October 2014
Knee flexion contractures prevent the full extension of the knee joint and cause disability. The etiology is not well defined. Extended periods of immobilization of joints lead to contractures difficult to completely reverse by rehabilitation treatments. Recovery of the complete range of motion without intervention has not been studied but is of importance to optimize clinical management. This study was designed to quantify the spontaneous reversibility of knee flexion contractures over time.
Knee flexion contractures of increasing severities were induced by internally fixing one knee of 250 adult male rats for 6 increasing durations. The contractures were followed for four different durations of spontaneous recovery up to 48 weeks (24 groups, target n = 10 per group). The angle of knee of extension at a standardized torque was measured. Contralateral knees constituted controls.
Full reversibility characterized by knee extension similar to controls was only measured in the lowest severity group where 4 weeks of spontaneous recovery reversed early-onset contractures. Spontaneous recovery of 2, 4 and 8 weeks caused partial gain of knee extension in longer-lasting contractures (P ≤ 0.05; all 4 comparisons). Extending the durations of spontaneous recovery failed to further improve knee extension (P > 0.05, all 12 comparisons). No reversal occurred in the highest severity group (32 week; P > 0.05).
Reversibility of knee flexion contractures was dependent on their severity. Full spontaneous recovery was limited to the least severe contractures. While contractures initially improved, a plateau was reached beyond which additional durations of spontaneous recovery led to no additional gain of knee extension. These results support our view that without treatment, permanent losses in knee mobility must be anticipated in immobility-induced contractures.
A contracture limits the passive range of motion of a joint and is caused by multiple factors that include joint immobility . Contractures are prevalent clinically as a consequence of casting, joint arthroplasty, sports injuries, bed rest and others [2–6]. Once established, joint contractures can limit function and performance .
Just how effective is spontaneous recovery in returning a contractured joint to normal range of motion? The recovery potential of joint contracture has been the focus of little research, restricted to animal experimentation of which very few studies reported quantitative data measured over time . In addition, published reports are controversial; some investigations in a rabbit model proposed that contractures left untreated may be fully reversible [8, 9]; this view was recently echoed . Other studies suggest the contrary: only contractures of recent onset have the potential to recover without treatment in the rat, rabbit and horse [11–15]. The potential for reversibility of joint contractures needs to be established.
Determining the reversibility of joint contractures in an animal model can have clinical relevance for patient care and for resource utilization. If contractures are largely reversible, treatment is not justified. If largely irreversible, delays in diagnosis or treatment may be costly since currently, there is no effective medical treatment to reverse or cure long-lasting joint contractures. For these reasons, a comprehensive reversibility experiment using a rat model and including quantitative measures of ROM over a time course would provide the rationale to improve the management of joint contractures.
In the current study, knee joint flexion contractures of various severities underwent incremental durations of spontaneous recovery in a rat model. The range of knee extension was measured using an automated goniometer. Our objectives were 1) to determine whether knee joint contractures are fully reversible during recovery without treatment and 2) to determine the duration of spontaneous recovery that produces maximal partial reversibility of joint contractures. Our hypotheses were that 1) while early-onset knee flexion contractures may be reversible, long-lasting contractures do not fully reverse spontaneously and 2) while initial partial reversibility may occur, extending the duration of recovery does not lead to further gains in knee extension.
This study provides evidence-based guidelines on the timing of intervention to manage joint contractures of various severities by accurately predicting their natural course.
We used SPSS 20.0 (IBM, Armonk, New York) for statistical testing. The data was compared in two ways. First, to detect a difference in mean knee range of extension between the experimental and contralateral knees; we ran 1-tail t-tests for the 24 experimental groups assuming the experimental knees would have a lower mean angle of extension than contralateral knees. A P value of ≤0.05 was interpreted as statistically significant: contractures had not fully reversed. Second, to detect the duration of recovery that improved contracture: the mean angle of extension of the experimental knees after each duration of recovery was compared to that at the previous duration of recovery using univariate analyses (recovery 1 vs 0, 2 vs 1, and 3 vs 2). A P value of ≤0.05 after Bonferroni correction (given the same rats were included in two different analyses) was interpreted as statistically significant: a plateau had not been achieved and this additional duration of recovery improved the contracture. Third, we tested for a change with time in the contralateral knee using an ANOVA for each contracture severity; a P value of ≤0.05 was interpreted as statistically significant. A confidence level of 95% was used on all analyses.
Thirteen rats required local wound care, of which 12 received antibiotics; all 13 were treated and included. At endpoint, data for 12 animals were not analyzed for persistent fibrous adhesions, leg fracture during testing, extension angle over 195° or images not recorded. Final number of experimental animals was 238; the distribution per group, fixation and recovery durations are shown in Figure 1. The animal model created contractures of various severities with maximal knee extension reaching 121° (101–140), 119° (96–133), 101° (77–136), 83° (75–93), 76° (60–103) and 76° (59–94) after 1, 2, 4, 8, 16 and 32 weeks of internal fixation, respectively (P = .003 for first comparison and <0.001 for the remaining 5 comparisons to contralateral knees; Figure 1).Full reversal occurred after 4 weeks of recovery in 1-week-old contracture. At all other durations of recovery, the mean angle of knee extension was smaller than contralateral knees (Figure 1). Thus, except for 1-week-old contractures, a full spontaneous reversal of the knee contracture was not observed in any joint contracture.
Durations of recovery that produced partial reversal of knee contracture were identified. Recovery for 2, 4, 8 and 8 weeks led to partial reversal of the contracture caused by 2, 4, 8 and 16 weeks of fixation, respectively (respective p-values of: .052, <.001, .048 and .009; Figure 1). The extent of partial gain was 15°, 31°, 15° and 20° for initial contractures of 44°, 40°, 59° and 70° respectively (Figure 1). These constituted a plateau after which additional durations of recovery added no significant gain in knee extension compared with the previous recovery duration (P > 0.05 for all 8 comparisons; Figure 1). Specifically, doubling the duration of recovery added no knee extension compared to the duration of recovery equal to the duration of fixation in all applicable groups (P > 0.05 for all 4 comparisons; Figure 1), and quadrupling the duration of recovery, when feasible, added no gain in knee extension compared to recovery double the duration of fixation (P > 0.05 for all 3 comparisons; Figure 1).
Recovery durations of 16, 32 or 48 weeks after 32 weeks of surgical fixation did not change the mean angles of knee extension (P > 0.05 for all 3 comparisons; Figure 1).Knees contralateral to the experimental knees showed a decrease in angle of extension over the duration of recovery for groups with unilateral fixation durations of 1, 2, 4 and 32 weeks (respective p-values of: .002, <.001, p < .001 and p = .005; Figure 1).
Spontaneous recovery of rat knee contractures for 4 weeks allowed full reversal of 1-week-old contractures. At all other contracture severities, knee flexion contractures were not fully reversible no matter the duration of recovery. These results confirm the first hypothesis that only recent-onset knee flexion contractures fully reverse spontaneously. This study confirmed reports of incomplete reversal of joint contractures in various animal models [11–16] and improved upon previous investigations by the broad range of 18 clinically relevant durations of spontaneous recovery, from adult to geriatric age, by standardized mechanical testing, and by sufficient sample size for statistical testing. These data should help resolve the controversy regarding the potential for full spontaneous recovery of joint contractures secondary to immobility [8, 10].Within a specific time window — recovery for 2–8 weeks, some knee joint contractures were partially reversible. The extent of partial reversal was modest, an average gain of 20° of knee extension. This corresponded to an average 41% of the contractures (Figure 1). These constituted plateaus in the spontaneous recovery; extending the duration of recovery never led to a significant improvement of the contractures, which confirms the second hypothesis. Once a joint contracture is diagnosed, the current study implies that simple observation is not an appropriate option. Our data predict a poor prognosis, with recovery of only 20° (or 41%) of the contracture. Therefore intervention may be necessary to regain knee extension beyond natural recovery.
This study also measured decreased knee extension over time in the rat knees contralateral to a contractured knee (Figure 1). In this animal model, at least three factors can contribute: both surgical procedures involved general anesthesia and postoperative recovery (approximately 1 week each). The temporary general hypomobility may have contributed to the loss of extension in contralateral knees. Secondly, an index contracture in the experimental leg may limit extension in contralateral knees to smoothen the gait pattern; patients with osteoarthritis and a unilateral knee flexion contracture lacked knee extension of the contralateral knee . Thirdly, aging remains a controversial contributor to decreased range of motion in diarthrodial joints [18–24]. In this study, using the contralateral knee joint for comparison controlled for the postoperative general hypomobility and for aging.
This animal model studied the simple immobility-induced joint contractures. No articular trauma, osteochondral damage or hemarthrosis accompanied the immobilization. No neurological injury altered the limb muscle tone (increased or decreased tone with an upper or a lower motor neuron injury, respectively) Importantly, no treatment was provided. This study produced normative data on the effects of joint immobility. The effects of other variables (partial mobility, articular trauma, change in neurological status, various treatments) need to be studied separately. Similarly, distinguishing the tissue limiting the knee joint, articular or muscular, can be obtained by comparing range of extension before and after myotomies .
The range of extension of the knee is a key variable in the biomechanical gait assessment. Full knee extension reaching 180 degrees is necessary for normal walking in humans. The magnitude of the knee flexion contracture proportionately disturbs the gait pattern. In humans, knee joint contractures required more quadriceps force for stability, caused a shorter stride length, increased oxygen consumption and negatively impacted balance and risk of falling [26–28]. For some patients, the immobility of normal joints is temporary (e.g., brace, cast, intensive care stay), and eventually they will actively use their joints again. The current study in the rat model shows that a statistically significant lack of knee extension remained despite long periods of spontaneous recovery similar to patients with fixed knee flexion contractures (Figure 3). The quantitative results measured over a time course of unassisted recovery in the current study may apply to patients with knee flexion contracture caused by prolonged immobility: after 2 weeks of immobility, their joints may develop a contracture that will not be fully reversible without treatment .
In the current study, 2 weeks of knee joint fixation had already caused contractures, not completely reversible without intervention. Early surveillance and intervention may decrease the risk of contractures . A survey found that, of patients spending 2 weeks or more in an intensive care unit, 26% never had their joint motion documented . Similar to the current experimental results, the development of contractures was linked to the duration of immobility: patients staying 8 weeks or longer in intensive care had an adjusted odds ratio of 7.1 to 1 of presenting a joint contracture compared to patients staying for 2–3 weeks . The current study confirmed that the longer a joint contracture remains undiagnosed or untreated, the more severe and irreversible the structural changes .
The animal model differed from clinical practice in that a brace or a cast causes less rigid immobility than the extraarticular fixation. Clinically, spasticity or musculoskeletal lesions often accompanies joint contractures in stroke, spinal cord injury, peripheral nerve injuries, trauma [2, 6]. Finally, in clinical practice some form of treatment may be instituted when a contracture is diagnosed.
First, the quadruped gait of the rat permitted long-term tolerance of the knee fixation in flexion because they never walk with their knee in full 180° of extension. However, functionally, a knee extension deficit in the rat is different than in humans . Whereas the basic mechanical data from this study may apply to other diarthrodial joints, the functional impact of contractures varies from joint to joint. Second, we did not study the effect of recovery beyond a fourfold duration of fixation, because we had already reached the life expectancy of some animals. Whether extended periods of recovery would have been beneficial is unlikely, since doubling or quadrupling the duration of recovery did not lead to any significant gain in knee extension. Finally, short durations of recovery after long periods of fixation may have shown that the plateau in partial reversal was achieved earlier than after 8 weeks of recovery.
In this animal model knee flexion contractures of 2-week onset or longer did not fully recover when untreated. Spontaneous recovery initially allowed modest, partial range of knee extension before a plateau was reached; extending the duration of spontaneous recovery was an ineffective intervention. In the absence of treatment, permanent losses in knee extension must be anticipated.
This research was performed in the Bone and Joint Research Laboratory at the University of Ottawa and supported by Canadian Institute of Health Research grant MOP 97831. None of the authors has a conflict of interest in regards to this research. The authors would like to acknowledge Joao Tomas and Tony Zandbelt for the arthrometer, Ying Nie for the surgeries with Sophie C. Bérubé, Li Dong and Hakim Louati, veterinarian staff, Khaoula Louati for image processing, Elizabeth Coletta for data analysis and graphs, Tim Ramsay for statistical review and Gloria Baker for editing the manuscript.
- Dudek N, Trudel G: Joint Contractures. Essentials of Physical Medicine and Rehabilitation: Musculoskeletal Disorders, Pain, and Rehabilitation. Edited by: Frontera WR, Silver JK, Rizzo TDJr. 2008, Philadelphia: Saunders, 651-655. Chapter 117, 2View ArticleGoogle Scholar
- Bushby K, Finkel R, Birnkrant DJ, Case LE, Clemens PR, Cripe L, Kaul A, Kinnett K, McDonald C, Pandya S, Poysky J, Shapiro F, Tomezsko J, Constantin C: Diagnosis and management of Duchenne muscular dystrophy, part 2: implementation of multidisciplinary care. Lancet Neurol. 2010, 9: 177-189. 10.1016/S1474-4422(09)70272-8.View ArticlePubMedGoogle Scholar
- Clavet H, Hébert PC, Fergusson DA, Doucette S, Trudel G: Joint contractures following prolonged stay in the intensive care unit. CMAJ. 2008, 178: 691-697. 10.1503/cmaj.071056.View ArticlePubMedPubMed CentralGoogle Scholar
- Fox P, Richardson J, McInnes B, Tait D, Bedard M: Effectiveness of a bed positioning program for treating older adults with knee contractures who are institutionalized. Phys Ther. 2000, 80: 363-372.PubMedGoogle Scholar
- Huang T, Blackwell SJ, Lewis SR: Ten years of experience in managing patients with burn contractures of axilla, elbow, wrist and knee joints. Plast Reconstr Surg. 1978, 61: 70-76. 10.1097/00006534-197801000-00012.View ArticlePubMedGoogle Scholar
- Singer BJ, Dunne JW, Singer KP, Jegasothy GM, Allison GT: Non-surgical management of ankle contracture following acquired brain injury. Disabil Rehabil. 2004, 26: 335-345. 10.1080/0963828032000174070.View ArticlePubMedGoogle Scholar
- Trudel G, Uhthoff HK, Brown M: Extent and direction of joint motion limitation after prolonged immobility: an experimental study in the rat. Arch Phys Med Rehabil. 1999, 80: 1542-1547. 10.1016/S0003-9993(99)90328-3.View ArticlePubMedGoogle Scholar
- Akeson WH, Woo SL, Amiel D, Doty DH: Rapid recovery from contractures in rabbit hindlimbs: a correlative biomechanical and biochemical study. Clin Orthop Relat Res. 1977, 122: 359-365.PubMedGoogle Scholar
- Haapala J, Arokoski JPA, Hyttinen MM, Lammi M, Tammi M, Kovanen V, Helminen H, Kiviranta I: Remobilization does not fully restore immobilization induced articular cartilage atrophy. Clin Orthop. 1999, 362: 218-229.View ArticlePubMedGoogle Scholar
- Hildebrand KA, Sutherland C, Zhang M: Rabbit knee model of post-traumatic joint contractures: the long-term natural history of motion loss and myofibroblasts. J Orthop Res. 2004, 22: 313-320. 10.1016/j.orthres.2003.08.012.View ArticlePubMedGoogle Scholar
- Ando A, Suda H, Hagiwara Y, Onoda Y, Chimoto E, Itoi E: Remobilization does not restore immobilization-induced adhesion of capsule and restricted joint motion in rat knee joints. Tohoku J Exp Med. 2012, 227: 13-22. 10.1620/tjem.227.13.View ArticlePubMedGoogle Scholar
- Finsterbush A, Friedman B: Early changes in immobilized rabbits knee joints: a light and electron microscopic study. Clin Orthop. 1973, 92: 305-319.View ArticlePubMedGoogle Scholar
- Trudel G, Zhou J, Uhthoff HK, Laneuville O: Four weeks of mobility after 8 weeks of immobility fails to restore normal motion: a preliminary study. Clin Orthop Relat Res. 2008, 466: 1239-1244. 10.1007/s11999-008-0181-z.View ArticlePubMedPubMed CentralGoogle Scholar
- Usuba M, Akai M, Shirasaki BS, Miyakawa S: Experimental joint contracture correction with low torque–long duration repeated stretching. Clin Orthop Relat Res. 2007, 456: 70-78.View ArticlePubMedGoogle Scholar
- Van Harreveld PD, Lillich JD, Kawcak CE, Gaughan EM, McLaughlin RM, DeBowes RM: Clinical evaluation of the effects of immobilization followed by remobilization and exercise on the metacarpophalangeal joint in horses. Am J Vet Res. 2002, 63: 282-288. 10.2460/ajvr.2002.63.282.View ArticlePubMedGoogle Scholar
- Katalinic OM, Harvey LA, Herbert RD: Effectiveness of stretch for the treatment and prevention of contractures in people with neurological conditions: a systematic review. Phys Ther. 2011, 91: 11-24. 10.2522/ptj.20100265.View ArticlePubMedGoogle Scholar
- Campbell TM: MS Thesis. Demographics and Posterior Knee Capsule Histologic and Genetic Characterization in Patients with Severe Knee Osteoarthritis: Comparing those with Contracture to those without Contracture. 2012, University of Ottawa, Department of Medicine, Available from [http://hdl.handle.net/10393/23176]Google Scholar
- James B, Parker AW: Active and passive mobility of lower limb joint in elderly men and women. Am J Phys Med Rehabil. 1989, 68 (4): 162-167. 10.1097/00002060-198908000-00002.View ArticlePubMedGoogle Scholar
- Barnes CJ, Van Steyn SJ, Fischer RA: The effects of age, sex, and shoulder dominance on range of motion of the shoulder. J Shoulder Elbow Surg. 2001, 10 (3): 242-246. 10.1067/mse.2001.115270.View ArticlePubMedGoogle Scholar
- Chapleau J, Canet F, Petit Y, Sandman E, Laflamme GY, Rouleau DM: Demographic and anthropometric factors affecting elbow range of motion in healthy adults. J Shoulder Elbow Surg. 2013, 22: 88-93. 10.1016/j.jse.2012.05.028.View ArticlePubMedGoogle Scholar
- Escalante A, Lichtenstein MJ, Hazuda HP: Determinants of shoulder and elbow flexion range: results from the San Antonio Longitudinal Study of Aging. Arthr Care Res. 1999, 12: 277-286. 10.1002/1529-0131(199908)12:4<277::AID-ART6>3.0.CO;2-5.View ArticleGoogle Scholar
- Lin CC, Ju MS, Huang HW: Gender and age effects on elbow joint stiffness in healthy subjects. Arch Phys Med Rehabil. 2005, 86: 82-85.View ArticlePubMedGoogle Scholar
- Roach KE, Miles TP: Normal hip and knee active range of motion: the relationship to age. Phys Ther. 1991, 71 (9): 656-665.PubMedGoogle Scholar
- Doriot N, Wang X: Effects of age and gender on maximum voluntary range of motion of the upper body joints. Ergonomics. 2006, 49 (3): 269-281. 10.1080/00140130500489873.View ArticlePubMedGoogle Scholar
- Trudel G, Laneuville O, Coletta E, Goudreau L, Uhthoff UK: Quantitative and temporal differential recovery of articular and muscular limitations of knee joint contractures; results in a rat model. J Appl Physiol. 2014, 117 (7): 730-737. 10.1152/japplphysiol.00409.2014.View ArticlePubMedGoogle Scholar
- Goudie ST AHD, Ahmad A, Maheshwari R, Picard F: Flexion contracture following primary total knee arthroplasty: risk factors and outcomes. Orthopedics. 2011, 34: e855-e859.PubMedGoogle Scholar
- Perry J, Antonelli D, Ford W: Analysis of knee-joint forces during flexed-knee stance. J Bone Joint Surg Am. 1975, 57 (7): 961-967.PubMedGoogle Scholar
- Campbell J, Waters RL, Thomas L, Lombardi R, Mayer C: Simulated knee contracture: demands of walking. Phys Ther. 1984, 64: 715-Google Scholar
- Needham DM: Mobilizing patients in the intensive care unit: improving neuromuscular weakness and physical function. JAMA. 2008, 300 (14): 1685-1690. 10.1001/jama.300.14.1685.View ArticlePubMedGoogle Scholar
- Clavet H, Hébert PC, Fergusson DA, Doucette S, Trudel G: Joint contractures in the intensive care unit: association with resource utilization and ambulatory status at discharge. Disabil Rehabil. 2011, 33: 105-112. 10.3109/09638288.2010.486468.View ArticlePubMedGoogle Scholar
- Trudel G, Seki M, Uhthoff HK: Synovial adhesions are more important than pannus proliferation in the pathogenesis of knee joint contracture after immobilization: an experimental investigation in the rat. J Rheumatol. 2000, 27: 351-357.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/15/338/prepub
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