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Inactivated autograft–prosthesis composite have a role for grade III giant cell tumor of bone around the knee
© Xu et al.; licensee BioMed Central Ltd. 2013
Received: 26 January 2013
Accepted: 28 October 2013
Published: 9 November 2013
Giant cell tumors (GCT) around the knee are common and pose a special problem of reconstruction after tumor excision, especially for grade III GCT. We questioned whether en bloc resection and reconstruction with alcohol inactivated autograft-prosthesis composite would provide (1) local control and long-term survival and (2) useful limb function in patients who had grade III GCT around the knee.
We retrospectively reviewed eight patients (5 males and 3 females) treated with this procedure with mean age of 31 years (range 20 to 43 years) from Jan 2007 to Oct 2008. 5 lesions were located in distal femur and 3 in proximal tibia. 4 patients were with primary tumor and the other 4 with recurrence. 2 patients showed pathological fracture.
Mean Follow-up is 54 months ranging from 38 to 47 months. No recurrence, metastasis, prosthesis loosening were found. The mean healing time between autograft and host bone was 5.5 months. The mean MSTS score was 26.3 (88%) ranging from 25 to 29. The mean ISOLS composite graft score was 32.8 (88.5%) ranging from 28 to 35. Creeping substitution is possibly the main way in bony junction. The healing time in femoral lesion is faster than that in tibial lesion.
The technique of alcohol inactivated autograft-prosthesis composite could be able to achieve satisfactory oncological and functional outcomes in Grade III GCT.
Giant cell tumor (GCT) of bone is aggressive, potentially malignant lesion which remains a difficult and challenging management problem. The ends of long bones in skeletally mature individuals are involved in more than 80% of cases and 75% of them occur around the knee joint . The reconstruction of large bone defects and restoration of joint function resulting from resection of aggressive grade III GCT around knee remains a challenging problem.
Since the local behavior of giant cell tumors can be aggressive and they have a greater risk of local recurrence, some authors advocate en bloc resection and reconstruction for these grade III lesions from the point of view of preventing local recurrence rate and preserving joint function [2, 3]. Three most popular reconstructive methods are endoprosthesis, allograft–prosthesis composite and biological reconstruction. However, each of these methods has its short- and long-term advantages and disadvantages . We noted that mega-prosthesis is a feasible method for treating GCT of grade III around the knee, and complications related to which were mainly prosthesis loosening and limb shortening, increase gradually with longer survival time .
We questioned whether en bloc resection and reconstruction with alcohol inactivated autograft-prosthesis composite would provide (1) local control and long-term survival and (2) useful limb function in patients who had grade III GCT around the knee. To overcome possible disadvantages of the above methods, 8 patients with grade III GCT around knee were treated with this method. The objectives were to evaluate whether this method can provide both mechanical stability in early time and fine limb function in long time on the basis of complete resection and decreasing recurrence rate.
Collection of retrospective clinical data and the publication of the data were in accordance with local guidelines for research ethics and were approved by General Hospital of Ji'Nan Military Region. All patients had been treated the procedure by the same chief surgeon (XCY) and his assistants (SFX and MX) in the same institute. All procedures were in compliance with the Helsinki Declaration.
Details of grade III GCT patients treated with autograft-prosthesis composite
Status of tumor
MSTS function score
ISOLS image score
Status at latest follow-up
Left distal femur
Right distal femur
Left distal femur
Left distal femur
Left distal femur
Right proximal tibia
Left proximal tibia
Right proximal tibia
The indications for a wide resection were extensive tumor (Campanacci III ) invasion with or without pathological fracture and rapid or large recurrent tumor after intensive curettage. In addition, according to Yang’s study , if the diameter of GCT lesion around the knee is over 1/2 on CT transversal image, en bloc resection and reconstruction should be chosen. All patients were in line with the above criteria.
Prophylactic antibiotics were administered for 48 hours. Low-molecular-weight heparin was administered for 2 weeks. For postoperative treatment, patients with lesion in distal femur were placed on bed for 2 weeks and in proximal tibia for 3 weeks. After that, partial weight bearing was allowed initially, and then weight bearing using two elbow crutches was allowed. Full weight bearing with no support was allowed at the end of 3 months. Plain anteroposterior and lateral radiographic examinations were done every 3 months for two years, bi-annually for a further three years and annually thereafter.
Limb function was evaluated with the Musculoskeletal Tumor Society (MSTS) rating scales, which comprise six items, namely pain, function, emotional acceptance, supports, walking and gait. The highest possible score is 30 and 5 points being allocated to each item . Bone healing features were evaluated with the International Society of Limb Salvage (ISOLS) composite grafts evaluation method, which assesses bone remodeling, interface, anchorage, implant, fusion, resorption, fracture, graft shortening, fixation on plain X-ray radiographs and rates these items from 1 to 4 points .
All operations were performed successfully and the mean operating time was 2 hours and a half. No intraoperative complications occurred and the mean bleeding amount was about 450 ml.
The mean follow-up was for 54 months ranging from 38 to 47 months. 6 were followed for more than 4 years, and 2 were for more than 5 years. No recurrence, metastasis or composite removal occurred at the end of follow-up. No infection, composite fracture or prosthesis loosening was found. All patients were disease-free.
The ideal aim in the management of GCT is to eradicate the tumor and preserve joint function . Wide resection is the treatment of choice, especially for GCT with extensive destruction of bone structure, recurrence, pathological fractures and difficulty in reconstruction after intralesional curettage [7, 10]. Progress in biomedical engineering along with better surgical techniques has improved overall 10-year prosthetic survival rate after endoprosthetic replacement from 20% to 80% in the past three decades. As the survival of prosthesis improves, the major concern is its reconstruction longevity. However, it is difficult to overcome the mentioned disadvantages of prosthesis. It was believed by us that the alcohol inactivated autograft-prosthesis composite could be chosen for Campanacci grade III GCT of which the lesion diameter was over 1/2 on CT transversal image, with or without recurrence and pathological fracture.
The main aims of bone and joint reconstruction are: (1) skeletal stabilization, (2) restoration of length and alignment, and (3) preservation or reconstruction of optimum function . It has been recommended that the most effective and easiest way to reconstruct the extensor mechanism sometimes around the knee is to use an allograft-prosthetic composite .
Achieving complete ablation of the tumor and preserving a functional extremity at the same time proves to be a difficult task due to the various anatomical factors unique to this site. Difficulties in the local control of giant cell tumors with Campanacci III as well as high rate of local recurrence following initial surgery have led the investigators to use different surgical modalities of reconstruction. At present, the most popular biological reconstruction method following skeletal tumor resection is allografting [13–16]. However, there are problems relating to infectious transmission, immunological reaction and refusal based on social or religious beliefs especially in Asian countries. Under these circumstances, recycled tumor bone autografts are widely used as an alternative to bone allografts. Devitalized bone autograft is particularly well suited in the region where allografts are not readily available . Techniques that are capable of destroying tumor cells in resected bone include (1) irradiation [14, 16], (2) autoclaving , (3) pasteurization [19, 20], (4) freezing-thawing with liquid nitrogen  and alcohol inactivation [22, 23]. Harrington et al.  reported that the autoclaving technique preserved a graft strong enough to support a joint arthroplasty and to allow early weight-bearing with a low risk of pseudarthrosis or late fatigue fracture. Jeon et al.  treated 15 distal femoral osteosarcoma patients who underwent reconstructions using pasteurized autograft–prosthesis composite. They found union of the osteotomy site was identified in ten patients (67%) of whom the average union time for junction sites was 16 months. Nonunion was identified in five patients, who had cemented fixation and a relatively long stem length in host bone. Loosening of the stem was found in three patients. Resorption of host bone was detected in two patients with noncemented fixation. Biau et al.  reported on irradiated allograft-prosthesis composites for proximal tibia reconstruction, it yielded poor results for proximal tibial reconstruction as complications and failures were common.
Literature review of autograft/allograft-prosthesis composite for neoplasms around knee
Number of patients
Average follow-up (months)
Type of tumor
Type of reconstruction
MSTS functional score
Song (2012) 
Pasteurized autograft-prosthesis composite
Gilbert (2009) 
Donati (2008) 
56 malignant tumors
2 failure operation
Jeon (2007) 
Pasteurized autograft-prosthesis composite
Biau (2007) 
Irradiated allograft-prosthesis composite
Wunder (2001) 
Irradiated allograft-prosthesis composite
The disadvantage of alcohol inactivated bone is that it needs a long time to accomplish revascularization and to integrate with surrounding bone. The rationality of alcohol inactivation is that alcohol could devitalize the tumor bone shell. The tumor cells had been devitalized when the ingrowth of surrounding vessels occurre . Our previous studies showed that continuous bone callus presented after 8 weeks and complete bony healing showed after 12 weeks in rabbit femur . The irradiated allograft presented the likely bony healing process of creeping substitution with the bone formation rate of 1 cm per 10 months . In this study, according to the dynamic imaging observation and ISOLS composite scoring, the new bone originated from host bone and the bone healing time in femur is about 4 to 6 months and that in tibia about 6–8 months. We hold the viewpoint that creeping substitution is possibly the main way in bony junction and the healing time in femur is faster than that in tibia.
Metal prosthesis resulted in artifacts during CT and MRI imaging, which impaired the evaluation of bone healing. On this consideration, X-ray imaging was chosen to evaluate bone healing according to ISOLS composite grafts evaluation method . The mean ISOLS composite graft score was 32.8 (88.5%) as good in our group ranging from 28 to 35 which were all over 28 as better. The average union time was 5.5 months (range 4–7.5 months) which was better than that of allograft (7.4 months)  and pasteurized autograft (16 months) . The alcohol inactivated autograft was superior to allograft in bone healing. In addition, it was recommended by us that good anatomic reduction and specially made long stem play important roles in bone healing. If the lesion extent was over half of the affected bone and the host bone with enough strength, the patient should undergo en bloc resection of tumor and reconstruction with alcohol inactivated autograft–prosthesis composite.
There are limitations in this study, such as small amount of patients and the less than 5 years’ follow-up, which might result in biased result. The multi-centric randomized controlled study with large samples should be considered.
Based on our outcomes, we believe alcohol inactivated autograft-prosthesis composite is a reasonable option as a limb salvage procedure for Grade III GCT around knee. By using this technique we have been able to achieve satisfactory oncological and functional outcomes in these patients.
Written informed consent was obtained from the patient’s guardian/parent/next of kin for the publication of this report and any accompanying images.
- Ghert MA, Rizzo M, Harrelson JM: Giant-cell tumor of the appendicular skeleton. Clin Orthop Relat Res. 2002, 400: 201-210.View ArticlePubMedGoogle Scholar
- Prosser GH, Baloch KG, Tillman RM: Does curettage without adjuvant therapy provide low recurrence rates in giant-cell tumors of bone?. Clin Orthop Relat Res. 2005, 435: 211-218.View ArticlePubMedGoogle Scholar
- Lackman RD, Hosalkar HS, Ogilvie CM: Intralesional curettage for grades II and III giant cell tumors of bone. Clin Orthop Relat Res. 2005, 438: 123-127.View ArticlePubMedGoogle Scholar
- Vidyadhara S, Rao SK: Techniques in the management of juxta-articular aggressive and recurrent giant cell tumors around the knee. Eur J Surg Oncol. 2007, 33 (2): 243-251. 10.1016/j.ejso.2006.05.023.View ArticlePubMedGoogle Scholar
- Yu X, Xu M, Song R: Long-term outcome of giant cell tumors of bone around the knee treated by en bloc resection of tumor and reconstruction with prosthesis. Orthopaedic Surgery. 2010, 2 (3): 211-217. 10.1111/j.1757-7861.2010.00089.x.View ArticlePubMedGoogle Scholar
- Campanacci M, Baldini N, Boriani S: Giant-cell tumor of bone. J Bone Joint Surg. 1987, 69 (1): 106-114.PubMedGoogle Scholar
- Yang ZM, Tao HM, Yang DS: The choice strategy of surgical treatment for giant cell tumor close to the knee. Chinese J Sur. 2006, 44 (24): 1693-1698.Google Scholar
- Enneking WF, Dunham W, Gebhardt MC: A system for the functional evaluation of reconstructive procedures after surgical treatment of tumors of the musculoskeletal system. Clin Orthop Relat Res. 1993, 286: 241-246.PubMedGoogle Scholar
- Glasser DFL: The ISOLS radiological implants evaluation system. Limb Salvage: Major Reconstructions in Oncologic and Nontumoral Conditions. Edited by: Langlais FBT. 1991, Berlin: Springer-Verlag, S23-Google Scholar
- Eckardt JJ, Grogan TJ: Giant cell tumor of bone. Clin Orthop Relat Res. 1986, 204: 45-58.PubMedGoogle Scholar
- Nishida J, Shimamura T: Methods of reconstruction for bone defect after tumor excision: a review of alternatives. Med Sci Monit. 2008, 14 (8): RA107-RA113.PubMedGoogle Scholar
- Lietman SA, Joyce MJ: Bone sarcomas: Overview of management, with a focus on surgical treatment considerations. Cleve Clin J Med. 2010, 77 (Suppl): 1S8-1S12.Google Scholar
- Gilbert NF, Yasko AW, Oates SD: Allograft-prosthetic composite reconstruction of the proximal part of the tibia. An analysis of the early results. J Bone Joint Surg. 2009, 91 (7): 1646-1656. 10.2106/JBJS.G.01542.View ArticlePubMedGoogle Scholar
- Biau DJ, Dumaine V, Babinet A: Allograft-prosthesis composites after bone tumor resection at the proximal tibia. Clin Orthop Relat Res. 2007, 456: 211-217.View ArticlePubMedGoogle Scholar
- Donati D, Colangeli M, Colangeli S: Allograft-prosthetic composite in the proximal tibia after bone tumor resection. Clin Orthop Relat Res. 2008, 466 (2): 459-465. 10.1007/s11999-007-0055-9.View ArticlePubMedPubMed CentralGoogle Scholar
- Wunder JS, Leitch K, Griffin AM: Comparison of two methods of reconstruction for primary malignant tumors at the knee: a sequential cohort study. J Surg Oncol. 2001, 77 (2): 89-99. 10.1002/jso.1076. discussion 100View ArticlePubMedGoogle Scholar
- Muramatsu K, Ihara K, Miyoshi T: Stimulation of neo-angiogenesis by combined use of irradiated and vascularized living bone graft for oncological reconstruction. Surg Oncol. 2012, 21 (3): 223-229. 10.1016/j.suronc.2011.12.004.View ArticlePubMedGoogle Scholar
- Harrington KD, Johnston JO, Kaufer HN: Limb salvage and prosthetic joint reconstruction for low-grade and selected high-grade sarcomas of bone after wide resection and replacement by autoclaved [corrected] autogeneic grafts. Clin Orthop Relat Res. 1986, 211: 180-214.PubMedGoogle Scholar
- Jeon DG, Kim MS, Cho WH: Pasteurized autograft-prosthesis composite for distal femoral osteosarcoma. J Orthop Sci. 2007, 12 (6): 542-549. 10.1007/s00776-007-1173-7.View ArticlePubMedGoogle Scholar
- Song WS, Cho WH, Jeon DG: A comparison of tumor prosthesis implantation and pasteurized autograft-prosthesis composite for proximal tibial tumor. J Orthop Sci. 2012, 17 (4): 457-463. 10.1007/s00776-012-0224-x.View ArticlePubMedGoogle Scholar
- Tsuchiya H, Wan SL, Sakayama K: Reconstruction using an autograft containing tumour treated by liquid nitrogen. J Bone Joint Surg Br. 2005, 87-B (2): 218-225.View ArticleGoogle Scholar
- Yu X, Liu X, Zhou Y: Inactivated Bone Replantation with Preservation of the Epiphysis in Children with Osteosarcoma: Clinical Report of Two Cases. Chin-German J Clin Oncol. 2005, 4 (3): 167-170. 10.1007/s10330-004-0298-9.View ArticleGoogle Scholar
- Sung HW, Wang HM, Kuo DP: EAR method: an alternative method of bone grafting following bone tumor resection (a preliminary report). Semin Surg Oncol. 1986, 2 (2): 90-98. 10.1002/ssu.2980020205.View ArticlePubMedGoogle Scholar
- Yu XC, Liu XP, Zhou Y: Inactivated bone replantation with preservation of the epiphysis for osteosarcoma in children. Orthop J Chin. 2007, 15 (11): 811-813.Google Scholar
- Yu XC, Liu XP, Zhou Y: Influence of alcoholic devitalization and replantation with inactivated bone on knee function: Clinical and experimental study. Chin J Bone Tumor Bone Dis. 2004, 3 (6): 335-340.Google Scholar
- Poffyn B, Sys G, Van Maele G: Radiographic analysis of extracorporeally irradiated autografts. Skeletal Radiol. 2010, 39 (10): 999-1008. 10.1007/s00256-010-0889-1.View ArticlePubMedGoogle Scholar
- Tu CQ, Shen B, FX P: A composite of massive frozen allograft and prosthesis replacement used in limb salvage surgery. Chinese J Ortho. 2004, 24 (10): 604-608.Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/14/319/prepub
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