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Lack of associations between two previously identified susceptible single nucleotide polymorphisms of interleukin-23 receptor gene and ankylosing spondylitis: a replication study in a Chinese Han population
© Qian et al.; licensee BioMed Central Ltd. 2013
Received: 7 November 2012
Accepted: 10 June 2013
Published: 17 June 2013
The human leukocyte antigen (HLA)-B27 gene is considered to be a major gene associated with predisposition to ankylosing spondylitis (AS); however, studies have demonstrated that non-HLA-B27 genes also contribute substantially to the susceptibility to AS. Two single nucleotide polymorphisms (SNPs), rs1004819 and rs10889677, of the interleukin-23 receptor (IL-23R) gene have been shown to be associated with AS susceptibility in European populations. However, ethnicity factors contribute to population splitting and genetic variation, and ethnic-specific genetic association studies are needed to validate these associations in patients from different ethnic backgrounds. This study therefore aimed to replicate the associations between these two SNPs and AS susceptibility in a Chinese Han population.
A total of 195 AS patients and 203 normal controls were recruited in this study. Two IL-23R gene SNPs, rs1004819 and rs10889677 were selected. Genotyping was performed in all subjects using the TaqMan probe method. Genotype and allele frequencies were compared between AS patients and normal controls by χ2 tests.
There were no significant differences in either the genotype frequencies (TT 36.4%, TC 48.7% and CC 14.9% in AS patients; TT 35.0%, TC 50.0% and CC 15.0% in normal controls) or allele frequencies (T 60.8% and C 39.2% in AS patients; T 60.0% and C 40.0% in normal controls) of rs1004819 between AS patients and normal controls (P > 0.05). In addition, both the genotype frequencies (AA 51.3%, AC 43.1% and CC 5.6% in AS patients; AA 57.6%, AC 35.5% and CC 6.9% in normal controls) and allele frequencies (A 72.8% and C 27.2% in AS patients; A 75.4% and C 24.6% in normal controls) of rs10889677 were also comparable between AS patients and normal controls (P > 0.05).
This study found no evidence for an association between either of the two previously identified AS-susceptibility IL-23R SNPs (rs1004819 and rs10889677) and onset of AS, indicating a possible difference in pathogenesis of AS between Chinese and European patients.
Ankylosing spondylitis (AS) is an autoimmune-related disease characterized by chronic inflammations and gradual ossifications of the spine and sacroiliac joints . Substantial evidence suggests that genetic predisposition has a significant impact on individual susceptibility to AS . Although the human leukocyte antigen (HLA)-B27 gene is considered to be a primary gene associated with predisposition to AS and confers disease susceptibility, it is unable to explain all cases of AS and its contribution to the overall genetic predisposition is only 20–30% . Increasing evidence suggests that other, non-HLA-B27 genes also play crucial roles in AS susceptibility [4–6].
The etiology of AS is characterized by aberrant regulations of both the adaptive and innate immune responses , suggesting that inflammatory mediators involved in the regulations of immunological reactions could be potential candidate genes. The interleukin-23 receptor (IL-23R) is a member of the hematopoietin receptor superfamily, and constitutes a specific component of the heterodimeric receptor for the cytokine IL-23 . IL-23R plays a key role in the differentiation of CD4 T-cell into TH17 lymphocytes, which mediate inflammations in several animal models of autoimmunity [9, 10]. IL-23R gene polymorphisms may thus be implicated in susceptibility to human autoimmune diseases, such as AS .
A recent genome-wide association study reported that the IL-23R gene conferred susceptibility to AS , while IL-23R gene variation was responsible for 9% of the population-attributable risks of AS . The associations between IL-23R gene polymorphisms and AS susceptibility have been confirmed in European populations by genetic association studies [13–15], though the genotype distributions may differ among patients with different ethnic backgrounds . Ethnic-specific genetic association studies are therefore needed to validate the associations between IL-23R gene polymorphisms and AS susceptibility. The aim of this study was to reevaluate the identified associations between two IL-23R gene single nucleotide polymorphisms (SNPs), rs1004819 and rs10889677, and AS susceptibility in a Chinese Han population.
The study population consisted of 195 sequential unrelated AS patients (183 men and 12 women) and 203 age- and gender-matched normal controls (178 men and 25 women) recruited from January 2008 to January 2012. All the subjects investigated were of Chinese Han ethnicity. All the AS patients were recruited from the outpatient clinic of the affiliated Drum Tower Hospital of Nanjing University Medical School, while the normal controls were recruited from the medical control center for the healthy population at the hospital. The average age of the AS patients was 32 years (range, 21–53 years), and all patients satisfied the modified 1984 New York criteria . The normal controls were healthy subjects with no autoimmune or musculoskeletal disease, with an average age of 37 years (range, 26–57 years). One hundred and ninety-two AS patients (98.5%) were HLA-B27-positive and all the normal controls were HLA-B27-negative.
Ethical approval was obtained from the research ethics committee of the medical school of Nanjing University and informed consent for DNA analysis was obtained from all subjects.
Sequences of primers and probes for SNP rs1004819 and rs10889677
Statistical analysis was performed using SPSS software (version 16.0, SAS, Inc, Chicago, IL, USA). The genotype and allelic frequencies in AS patients and normal controls were compared using χ2 tests. P < 0.05 was considered to be statistically significant.
The genotype frequencies of these two SNPs did not deviate significantly from the Hardy-Weinberg equilibrium in either the AS patients or controls.
Allele and genotype frequencies of SNP rs1004819 and rs10889677 in ankylosing spondylitis patients and normal controls
Genotype and Allele
AS patients N (%)
Normal controls N (%)
Allele frequencies of SNP rs10889677 in European and Chinese Han populations
The IL-23R gene encodes an important cytokine receptor in the Th17 subset of T lymphocytes [18, 19], and IL-23R, together with IL-12Rβ1, comprises the IL-23 receptor complex in IL-23-responsive cells . IL-23 is one of the major regulators of innate and adaptive immunities . It stimulates a unique CD4+ inflammatory T-cell characterized by secretion of IL-17, tumor necrosis factor and IL-6, which are strongly associated with proinflammatory responses and severe autoimmunity . These conclusions suggest a possible involvement of the IL-23/IL-23R pathway in the etiology of AS. SNP rs1004819 and SNP rs10889677 are located in the intronic region and 3’-untranslated (3’-UTR) region of the IL-23R gene, respectively . rs1004819 may exert its influence by regulating differential splicing of IL-23R mRNA, while rs10889677 may lead to variation in the 3’-UTR region, which could be associated with reduced binding of IL-23R mRNA . Both these variations may have functional consequences for the IL-23R pathway and could provide a possible explanation for the associations between these two variants and AS susceptibility in European populations.
Despite the positive associations between these two SNPs and AS in European populations, neither rs1004819 nor rs10889677 was found to be related to AS susceptibility in the current study. The genotype and allele frequencies of both SNPs were similar in AS patients and controls, indicating that neither variation in the IL-23R gene was relevant to the etiology of AS. These results suggest that the mechanism responsible for AS may differ between Chinese and European patients, and that AS in Chinese patients may develop through a mechanism independent of these two IL-23R gene variants. Our results support the idea that different disease mechanisms exist in different ethnic populations , and a different predisposition gene may be involved in the IL-23/IL-23R signaling pathway in Chinese AS patients. However, we only investigated two SNPs in our study, and therefore cannot completely exclude a role for IL-23R gene in the development of AS in Chinese populations. Further studies including other SNPs that cover the IL-23R gene are necessary to clarify its true role in AS susceptibility.
In conclusion, the results of this study differed substantially from those of previous European studies. We suggest that this discrepancy can be attributed to racial differences, indicating the need to perform genetic association studies in different ethnic populations to identify race-specific predisposition genes. However, the results of genetic association studies may also differ within races and may produce false-negative or false-positive results as a result of selection bias of the samples. Thus further studies in the Chinese Han population, including a larger sample size, are also required to confirm the effects of the SNP rs1004819 and SNP rs10889677 on the development of AS.
The authors would like to thank the Development of Medical Technology Key Project (Nanjing, China, ZKX10008) and Medical Technology Development Project (Nanjing, China, YKK09100) for their financial supports.
- Brown MA, Laval SH, Brophy S, Calin A: Recurrence risk modelling of the genetic susceptibility to ankylosing spondylitis. Ann Rheum Dis. 2000, 59: 883-886. 10.1136/ard.59.11.883.View ArticlePubMedPubMed CentralGoogle Scholar
- Reveille JD: Recent studies on the genetic basis of ankylosing spondylitis. Curr Rheumatol Rep. 2009, 11: 340-348. 10.1007/s11926-009-0049-6.View ArticlePubMedGoogle Scholar
- Santos FP, Bastos E, Ligeiro D, Mourao AF, Chaves R, Trindade H, Guedes-Pinto H, Branco JC: Genetic basis of ankylosing spondylitis. Acta Reumatol Port. 2007, 32: 243-252.PubMedGoogle Scholar
- Gunal EK, Sarvan FO, Kamali S, Gul A, Inanc M, Carin M, Konice M, Aral O, Ocal L: Low frequency of HLA-B27 in ankylosing spondylitis patients from Turkey. Joint Bone Spine. 2008, 75: 299-302. 10.1016/j.jbspin.2007.06.021.View ArticlePubMedGoogle Scholar
- Laval SH, Timms A, Edwards S, Bradbury L, Brophy S, Milicic A, Rubin L, Siminovitch KA, Weeks DE, Calin A: Whole-genome screening in ankylosing spondylitis: evidence of non-MHC genetic-susceptibility loci. Am J Hum Genet. 2001, 68: 918-926. 10.1086/319509.View ArticlePubMedPubMed CentralGoogle Scholar
- Brown MA: Breakthroughs in genetic studies of ankylosing spondylitis. Rheumatology (Oxford). 2008, 47: 132-137.View ArticleGoogle Scholar
- Rueda B, Orozco G, Raya E, Fernandez-Sueiro JL, Mulero J, Blanco FJ, Vilches C, Gonzalez-Gay MA, Martin J: The IL23R Arg381Gln non-synonymous polymorphism confers susceptibility to ankylosing spondylitis. Ann Rheum Dis. 2008, 67: 1451-1454. 10.1136/ard.2007.080283.View ArticlePubMedGoogle Scholar
- Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F: A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002, 168: 5699-5708.View ArticlePubMedGoogle Scholar
- Bettelli E, Oukka M, Kuchroo VK: T(H)-17 cells in the circle of immunity and autoimmunity. Nat Immunol. 2007, 8: 345-350.View ArticlePubMedGoogle Scholar
- Langrish CL, McKenzie BS, Wilson NJ, de Waal Malefyt R, Kastelein RA, Cua DJ: IL-12 and IL-23: master regulators of innate and adaptive immunity. Immunol Rev. 2004, 202: 96-105. 10.1111/j.0105-2896.2004.00214.x.View ArticlePubMedGoogle Scholar
- Safrany E, Melegh B: Functional variants of the interleukin-23 receptor gene in non-gastrointestinal autoimmune diseases. Curr Med Chem. 2009, 16: 3766-3774. 10.2174/092986709789104975.View ArticlePubMedGoogle Scholar
- Burton PR, Clayton DG, Cardon LR, Craddock N, Deloukas P, Duncanson A, Kwiatkowski DP, McCarthy MI, Ouwehand WH, Samani NJ: Association scan of 14,500 nonsynonymous SNPs in four diseases identifies autoimmunity variants. Nat Genet. 2007, 39: 1329-1337. 10.1038/ng.2007.17.View ArticlePubMedGoogle Scholar
- Karaderi T, Harvey D, Farrar C, Appleton LH, Stone MA, Sturrock RD, Brown MA, Wordsworth P, Pointon JJ: Association between the interleukin 23 receptor and ankylosing spondylitis is confirmed by a new UK case–control study and meta-analysis of published series. Rheumatology (Oxford). 2009, 48: 386-389. 10.1093/rheumatology/ken501.View ArticleGoogle Scholar
- Safrany E, Pazar B, Csongei V, Jaromi L, Polgar N, Sipeky C, Horvath IF, Zeher M, Poor G, Melegh B: Variants of the IL23R gene are associated with ankylosing spondylitis but not with Sjogren syndrome in Hungarian population samples. Scand J Immunol. 2009, 70: 68-74. 10.1111/j.1365-3083.2009.02265.x.View ArticlePubMedGoogle Scholar
- Pimentel-Santos FM, Ligeiro D, Matos M, Mourao AF, Sousa E, Pinto P, Ribeiro A, Sousa M, Barcelos A, Godinho F: Association of IL23R and ERAP1 genes with ankylosing spondylitis in a Portuguese population. Clin Exp Rheumatol. 2009, 27: 800-806.PubMedGoogle Scholar
- Lee YH, Choi SJ, Ji JD, Song GG: Associations between interleukin-23R polymorphisms and ankylosing spondylitis susceptibility: a meta-analysis. Inflamm Res. 2012, 61: 143-149. 10.1007/s00011-011-0398-2.View ArticlePubMedGoogle Scholar
- van der Linden S, Valkenburg HA, Cats A: Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria. Arthritis Rheum. 1984, 27: 361-368. 10.1002/art.1780270401.View ArticlePubMedGoogle Scholar
- Wilson NJ, Boniface K, Chan JR, McKenzie BS, Blumenschein WM, Mattson JD, Basham B, Smith K, Chen T, Morel F: Development, cytokine profile and function of human interleukin 17-producing helper T cells. Nat Immunol. 2007, 8: 950-957.View ArticlePubMedGoogle Scholar
- McKenzie BS, Kastelein RA, Cua DJ: Understanding the IL-23-IL-17 immune pathway. Trends Immunol. 2006, 27: 17-23. 10.1016/j.it.2005.10.003.View ArticlePubMedGoogle Scholar
- Hunter CA: New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat Rev Immunol. 2005, 5: 521-531. 10.1038/nri1648.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/14/190/prepub
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