Evaluation of a novel nanocrystalline hydroxyapatite paste Ostim® in comparison to Alpha-BSM® - more bone ingrowth inside the implanted material with Ostim® compared to Alpha BSM®
© Huber et al; licensee BioMed Central Ltd. 2009
Received: 10 May 2009
Accepted: 22 December 2009
Published: 22 December 2009
The purpose of this study was to evaluate the performance a newly developed nanocrystalline hydroxyapatite, OSTIM® following functional implantation in femoral sites in thirty-eight sheep for 1, 2 or 3 months. Ostim® 35 was compared to an established calcium phosphate, Alpha BSM®.
Biomechanical testing, μ-CT analysis, histological and histomorphological analyses were conducted to compare the treatments including evaluation of bone regeneration level, material degradation, implant biomechanical characteristics.
The micro-computed tomography (μCT) analysis and macroscopic observations showed that Ostim® seemed to diffuse easily particularly when the defects were created in a cancellous bone area. Alpha BSM® remained in the defect.
The performance of Ostim was good in terms of mechanical properties that were similar to Alpha BSM® and the histological analysis showed that the bone regeneration was better with Ostim® than with Alpha BSM®. The histomorphometric analysis confirmed the qualitative analysis and showed more bone ingrowth inside the implanted material with Ostim® when compared to Alpha BSM ® at all time points.
The successful bone healing with osseous consolidation verifies the importance of the nanocrystalline hydroxyapatite in the treatment of metaphyseal osseous volume defects in the metaphyseal spongiosa.
Operative reconstruction of bone defects beyond a certain size still remains a challenge to trauma and orthopedic surgeons. Every year, millions of people worldwide are suffering from bone defects arising from trauma, tumor or bone diseases. In approximately 10% of all traumatically related loss of bone structure or even tumor related bone defects, spontaneous bone healing is not able to restore the required physiological stability. In such cases bone replacement materials are often necessary to reconstruct the anatomical morphology and restore stability of the bone.
The use of autologous pelvic bone is still considered as the gold standard in the reconstruction of bone defects because of its unsurpassed biological activity even in implant sites with low osteogenic potential. Pelvic bone harvesting from the iliac crest does, however, presents unacceptable rates of morbidity at the grafting site and at the same time may only provide a limited amount of cancellous bone[2–8]. Chronic pain can be present in up to 39% of patients at the donor site after iliac crest harvesting. Other published complications include: fractures, infection, nerve and arterial injury.
Other bone sources include bone allografts which carry the potential of disease transmission, immunogenicity and possibly lower union rates[4, 9]. Furthermore, the structural, mechanical, and resorption properties of allografts are usually much altered by processing, preservation, and sterilization techniques[4, 10, 11]. The relative concerns over the use of either autograft or allograft have led to the development of numerous bone graft substitutes[12–20].
In the ideal case artificial bone replacement materials should present a similar structure and composition to human bone and thus be able to present bone function. The materials should be osteoconductive and osteoinductive by allowing osteoblast and osteoclast activity. At present there are over 100 approved bone replacement materials in Germany alone. The spectrum encompasses mainly hydroxyapatite ceramics, absorbable calcium phosphate cements, various metals, plastics and a variety of composites. The most commonly used synthetic mineral substitutes for bone defect and trauma applications as implant coatings and defect fillers are hydroxyapatite cements, which have already undergone comprehensive animal testing and have also established themselves in many surgical procedures on human patients[18–39].
Ostim® represents a brand new development among the purely synthetically produced and rapidly absorbable Hydroxyapatite compounds. It has been widely and successfully used in the fields of oral and maxillofacial surgery and orthopedic and trauma surgery[40–46].
The aim of the following study was to compare the newly developed Ostim® with tricalcium phosphate cement Alpha-BSM®, an already established bone replacement material, in relation to their biocompatibility and bone ingrowth in a bone defect.
Material properties of the hydroxyapatite compounds used
The product is supplied in a ready-to-use syringe to which a needle or a flexible 5 cm nozzle can be attached in order to inject the paste into deeper voids.
Alpha BSM® (ETEX Corp, CAMBRIDGE, MA 02139, USA), an established calcium phosphate cement, is an endothermically setting apatitic calcium phosphate bone substitute. The final product (means after the conversion reaction is complete) consists of purely crystalline HA with an average crystallite size of 12-14 nm. Alpha BSM® has a paste like consistency until it is injected and hardens at body temperature. In 20 min it achieves complete conversion and mechanical strength. The reported mechanical strength is 15 MPa. Specific surface area of the dried product is 78 m²/g.
None of two materials has macropores and both are applied as wet pastes. The water content of Ostim is 65%, freshly prepared alpha-BSM paste has a water content of 55%.
Animal experiment protocol
Permission was granted to conduct all parts of our animal experiments we applied for (authorization number 59036 by BIOMATECH Ethical Committee on March 16, 2007). The sheep model has been used in intra-osseous implantation studies and is recognized by International Regulatory organizations[48, 49]. Thirty eight with three in reserve (38 + 3) female sheep (Ovisaries), between 53 and 73 kg and between 2 and 5 years old were used for the animal experiments.
The evaluation of the performance and the biocompatibility of a bone substitute was tested following functional implantation in femoral sites in thirty-eight sheep for 1, 2 or 3 months (total of 76 sites). The Ostim® material (n = 38 sites) was compared to Alpha-BSM® (n = 38 sites) following implantation in a cancellous bone defect (see additional file 1).
Each animal received an implantation of each bone replacement material in the cancellous bone of the right and left medial distal femoral epiphysis respectively. The treatment allocation was randomly assigned between the right and left leg. Pre-medication and anesthesia were performed by intravenous injection of thiopental-pentobarbital mixture (NESDONAL®, MERIAL, France; pentobarbital sodique, CEVA Santé animale, France) and atropine (Atropinum sulfuricum, AGUETTANT, France) followed by inhalation of a O2 - isoflurane (1-4%) mixture. Each animal received an analgesic (flunixine, FINADYNE® Injectable, Schering-Plough, France) pre-operatively. As a prophylactic measure, perioperative antibiotics penicillin-procaine and penicillin benzathine (DUPHAPEN® LA, FORT DODGE, France) was given.
Using standard aseptic techniques, a cutaneous incision was made on the medial face of the knee. The medial condyle of the stifle joint of each animal was exposed by blunt dissection of the subcutaneous tissue and the fascia. The muscle and joint capsule were gently dissected and the supracondyle bone area was approached. The periosteum was carefully removed from the femoral epiphysis and metaphysis to expose the implant site. A core drill was used to drill a 10 mm diameter × 15 mm deep hole in the distal femoral epiphysis. After drilling the defect to the required depth, an extractor was inserted and driven down to the bottom of the defect. The extractor was turned to break the bone trabecula at the bottom of the defect. Depth of the defect was measured. Each defect was filled with either the test or the control article. The incision was then closed by closing the subcutaneous layers one by one with absorbable sutures (Vicryl 2-0 and Vicryl 1, ETHICON, France), and the skin layer was closed using surgical staples. The animal was repositioned and the other bone replacement material implanted in the same fashion. At the appropriate termination interval, the designated sheep were terminated by lethal injection of barbiturate (DOLETHALND, VETOQUINOL, France). A macroscopic examination of the implanted femoral epiphyses was performed. The epiphyses were then harvested and identified. The epiphyses from the 2 and 3 months animals were placed in 10% buffered formalin. After fixation, these samples were submitted for μ-CT analysis. The epiphyses from the 3 months interval were arbitrarily divided so that sixteen sites sampled from eight animals (eight test and eight control) were selected for biomechanical testing and the remaining sites were fixed in 10% buffered formalin.
Preparation of the samples
The test specimens were prepared so that the implantation site were in the centre of the sample. The tissue samples were provided in physiological serum and the trials were performed within 48 h after removal. Their thickness has been measured using a slide caliper at the implantation point.
First, the thickness of the samples was measured with a calliper. Following thickness measurements, the samples were immersed in room temperature saline solution. The samples were submitted to an indentation test. Load was applied axially to the specimens by a pin with a diameter of 4 mm and with a displacement speed of 0.4 mm/min until failure. To determine the mechanical characteristics of the samples, the data collected were stiffness and Young's modulus, yield point, maximum load and corresponding stress and strain. This biomechanical testing was also performed on four non implanted test articles.
Non implanted samples were prepared by placing Ostim samples in a cylindrical container of a diameter of 12 mm and a height of 20 mm. They were tested immediately thereafter.
Following fixation, the epiphyses sites for the 2 months animals and the ten animals designated from the 3 months interval were used to quantify the mean BV/TV (Bone + substitute volume/Total bone defect volume). The μCT measurements were conducted at ETH Zürich by means of a μCT 80, Scanco Medical AG, Switzerland. The measured data was filtered using a constrained Gaissian filter with finite filter support (1 Voxel) and filter width (σ = 1.2). The images were then binarized to separate the object from the background using a global thresholding procedure. For density analysis a cylinder of 11.7 mm in diameter and 17 mm length was analyzed. The cylinder dimension was obtained from the μCT images and was chosen to represent the original defect as good as possible. For this the cylinder was morphed along the axis of the defect zone in the following way: A circle with 11.7 mm was positioned over the defect zone on the first and the last μCT slice of the given range of the substitute (17 mm) and the cylinder was morphed in-between. Within this cylinder the apparent density was computed as the ratio of substitute versus background.
Each implant site and surrounding tissue was isolated using a band saw. After labelling and fixing in 10% buffered formalin solution, the sites were longitudinally divided in two blocks. One half of each site was dehydrated in alcohol solutions of increasing concentrations, cleared in xylene, decalcified and embedded in paraffin. Embedded sites were then longitudinally cut at 5 to 7 μm using a microtome (MICROM®, France). One paraffin section per site was prepared and stained with Masson's trichrome. The other half of each site was dehydrated in alcohol solutions at increasing concentrations, cleared in xylene and embedded in polymethylmethacrylate (PMMA). One longitudinal section was prepared using the Exakt system [25, 50]. The resin sections were stained with a modified Paragon.
Qualitative evaluation of implanted sites
Histological sections were observed using a Nikon microscope (Eclipse E600) fitted with ×4, ×10, ×25 and ×40 objectives. Semi-quantitative analysis was performed in accordance with ISO 10993-6 standard: local tolerance criteria and performance were analyzed and graded. (necrosis, fibrinous exudate, tissue degeneration, inflammatory cells, fibrous tissue, osteolysis, newly formed bone, osteointegration, osteoconduction, and material degradation.)
the neoformed bone, the direct bone/implant interface and the osteoconduction process
presence of fibrosis
The semi-quantitative scoring system is shown in additional file 2
Quantitative histomorphometry of implanted sites
The histological slides obtained from resin blocks were evaluated under a Zeiss Axioscope microscope fitted with ×5, ×10, ×20 and ×40 objectives and equipped with a color image analyzing system SAMBA® (SAMBA TECHNOLOGIES, France). Two standard areas of investigation were located peripherally to the Bone/Implant junction, and two standard areas of investigation were located internally to the Bone/Implant junction.
bone to implant contact, BIC (expressed in percentage)
bone density around the material (expressed in percentage)
bone density inside the material (expressed in percentage)
At each time point a statistical analysis (ANOVA method) was performed for these 3 parameters, considering control versus test article.
The statistical tests used for a comparison between groups for the main parameters of biomechanical testing and quantitative histological analysis were Mann and Whitney or ANOVA tests performed with a software (SPSS® version 14.0, edited by SPSS Inc, Chicago).
Biomechanical testing was performed on 16 samples from the 3 month time-period (see additional file 3). At yield point, the stress was higher in the Alpha-BSM® Group and the strain was higher in the Ostim® group. Resistance, stress and strain were higher in the Alpha-BSM® group. Elasticity, stiffness and Young modulus were higher in the Ostim® group. However, no statistically significant differences were observed for any parameters. The biomechanical testing performed on four non implanted test articles showed a maximum load for resistance and yield point at least ten times lower than the values observed after implantation, suggesting a significant hardening and/or bone ingrowth during the 3 month implantation period.
Microscopic analysis of femoral sites at 1 month (see additional file 4)
Microscopic analysis of femoral sites at 2 months (see additional file 4)
Microscopic analysis of femoral sites at 3 months (see additional file 4)
Quantitative histolomorphometrical analysis
At early time points, Ostim® showed much better microscopic signs of osteointegration, osteoconduction and overall bone ingrowth when compared to the Alpha-BSM®. After 2 and 3 months, the bone regeneration level within the Ostim® treated sites was better than the Alpha-BSM® treated sites, but new bone formation tended to slow down when compared to the steadier progression and fewer inter-individual variations observed with Alpha-BSM® (see additional file 5 and 6).
Bone to Implant Contact (BIC)
After 1 month, bone to implant contact was significantly different between alpha BSM and Ostim groups (p = 0.004). Bone to implant contact around the material was found higher in the alpha BSM than in the Ostim group. This difference was transient and not observed at later time-points (see additional file 7).
Bone density inside the material
Bone density around the material did not show statistically significant variations at all considered time-points.
Discussion and Conclusions
There were a number of attempts in the past to replace bone loss with bone replacement materials. Hamilton in 1881 used disinfected sponges and Gluck in 1891 implanted ivory into bone defects. Dreesmann even tried gypsum in 1892. In the 20th century Maatz experimented with bovine bone and Tarsoly filled the defects with egg shells. Modern bone replacement materials composed of calcium phosphate are either extracted from bovine cancellous bone or are produced synthetically[14, 18, 52, 53]. The requirements of an ideal bone replacement material have not changed over the past 30 years: simple application, osteoconductive properties, and complete resobarbility during bone ingrowth[53, 54].
Alpha-BSM® fulfils these requirements very well as a synthetic calcium phosphate bone replacement material. Compared to other calcium phosphates which require five years or more to be resorbed by the host bone[55, 56], Alpha BSM® appears to undergo more rapid resorption[22, 52]. It does, however, have less compressive strength than other types of cements . Compared to autologous cancellous bone, calcium phosphate cements have shown superior compressive strength. Welch et al reported a significantly lower secondary loss of correction with the use of Alpha-BSM®, in the treatment of tibia head fractures in goats compared to autologous spongiosa. Russell also revealed a significantly lower subsidence in tibia head fractures with the use of Alpha-BSM® compared to autologous bone grafts in the scope of a multicentre study with 119 Patients.
The cement microstructure of Alpha-BSM®, which results from a reaction of precipitation, is not equivalent to the crystalline structure of human bone[18, 57, 58]. The nanocrystalline structure of Ostim® on the other hand resembles human bone much closer (Calcium/Posphate ratio of 1,675 and specific surface area of 106 m2g-1 is synthesized by a wet chemical reaction of precipitation under permanent pH control using CaO dispersed in water under constant stirring to maintain a suspension state and H3PO4 as starting material [47, 58]. Ostim® has been observed as needle shaped HA crystals forming agglomerates in transmission electron microscopy [1, 47]. According to the hypotheses put forward by Constanz and Knaack such HA-compounds accelerate the bony ingrowth into the CSD as they closely mimic the required resorptive and osseointegrative properties of poor crystalline apatitic structure of natural bone[52, 57, 58]. Moreover Kilian et al also presented evidence of angiogenesis within bone defects filled with nanocrystalline hydroxyapatite [59, 1, 60]. These properties appear to explain the significantly increased ingrowth of bone of the Ostim® group compared to the Alpha-BSM® group. There was no significant difference between the two materials with regard to their biomechanical stability.
Ostim® 35 showed limited signs of degradation after 1 month but degradation increased over time and was of moderate grade after 3 months in our experimental series. This confirms the results obtained in a number of different experimental animal models of other studies. Grigoryan et al described the rapid bone ingrowth at a low complication rate following the treatment of jaw defects with Ostim® in dogs in 2000 . Our own animal experiments dealing with the filling of critical size defects with Ostim in New Zealand white rabbits also resulted in swift and uniform bone ingrowth. The results were further enhanced by the additional use of a sintered hydroxyapatite ceramic. Ostim® was always present in the bone after 60 days following implantation, as in the present study. Laschke reported a guided neovascularization directed toward areas of Ostim® degradation in Syrian golden hamsters . Spiess further confirmed the good biocompatibility, osteoconductivity and bone ingrowth in New Zealand whites, but reported a halt in the Ostim® degradation process 6 weeks following implantation.
Comprehensive clinical experience of using the hydrated HA-paste as a void filling exists in the field of maxillofacial surgery. Various stomatology publications describe an accelerated fracture healing and bone density increase at a high degree of tolerance. In 1996 Zuev treated 395 patients with jaw defects and peridontal abscesses. The complication rate of the 200 patients in the Ostim group was at 1.5% compared to 3.6% in the group with 195 patients treated with allografts. Bezrukow achieved excellent results in 1998 after treating 49 patients with Ostim® following cystectomy of benign cyst-tumours of the jaw. The defects in all 49 patients were replaced with fresh bone three months following the defect filling . Gerlachfilled 44 mandibular cysts with Ostim® and reported complete material resorption with an extemely low complication rate. Strietzel et al performed a lateral alveolar ridge augmentation with Ostim® in 14 patients. Six months later histological results showed good bone ingrowth of the defect and small amounts of Ostim® remnants .
Ostim® in combination with conventional or angularly stable osteosynthesis has also been successfully used in the field of traumatology. Particularly good results were achieved in prospective cohort studies with patients for the treatment of radial fractures, with closed intraarticular calcaneus fractures, as well as tibia compression fractures [40–42]. Histological examinations of bone harvested from patients during elective metal removal, have shown excellent bone ingrowth into the past bone defect with well structured cortical and cancellous bone tissue and without any inflammatory reaction orosteofibrosis. The implanted material was mainly resorbed.
Our data verifies the importance of the nanocrystalline hydroxyapatite in the treatment of metaphyseal osseous volume defects in the metaphyseal spongiosa, even though this study was done on an experimental animal model. Further clinical studies need to be conducted to validate the significance of Ostim in human bone defects. Due to its good biocompatibility and high rates of resorption we can see a number of indications for the use of Ostim in the field of traumatology. In our opinion the application of Ostim® should always be combined with some form of stable osteosynthesis, preferably with an angularly stable plate, due to its lack of dimensional stability.
- Schnettler R, Stahl JP, Alt V, Pavlidis T, Dingeldein E, Wenisch S: Calcium phosphat-based bone substitutes. European Journal of Trauma. 2004, 4: 219-229.Google Scholar
- Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA: Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res. 1996, 300-309. 10.1097/00003086-199608000-00037.Google Scholar
- Blakemore ME: Fractures at cancellous bone graft donor sites. Injury. 1983, 14: 519-522. 10.1016/0020-1383(83)90055-4.View ArticlePubMedGoogle Scholar
- Boyce T, Edwards J, Scarborough N: Allograft bone. The influence of processing on safety and performance. Orthop Clin North Am. 1999, 30: 571-581. 10.1016/S0030-5898(05)70110-3.View ArticlePubMedGoogle Scholar
- Goulet JA, Senunas LE, DeSilva GL, Greenfield ML: Autogenous iliac crest bone graft. Complications and functional assessment. Clin Orthop Relat Res. 1997, 76-81. 10.1097/00003086-199706000-00011.Google Scholar
- Ubhi CS, Morris DL: Fracture and herniation of bowel at bone graft donor site in the iliac crest. Injury. 1984, 16: 202-203. 10.1016/0020-1383(84)90162-1.View ArticlePubMedGoogle Scholar
- Younger EM, Chapman MW: Morbidity at bone graft donor sites. J Orthop Trauma. 1989, 3: 192-195. 10.1097/00005131-198909000-00002.View ArticlePubMedGoogle Scholar
- Wippermann BW, Schratt HE, Steeg S, Tscherne H: [Complications of spongiosa harvesting of the ilial crest. A retrospective analysis of 1, 191 cases]. Chirurg. 1997, 68 (12): 1286-1291. 10.1007/s001040050361.View ArticlePubMedGoogle Scholar
- Stevenson S: Biology of bone grafts. Orthop Clin North Am. 1999, 30 (4): 543-552. 10.1016/S0030-5898(05)70107-3.View ArticlePubMedGoogle Scholar
- Goldberg VM, Stevenson S: Natural history of autografts and allografts. Clin Orthop Relat Res. 1987, 7-16.Google Scholar
- Mankin HJ, Doppelt S, Tomford W: Clinical experience with allograft implantation. The first ten years. Clin Orthop Relat Res. 1983, 69-86.Google Scholar
- Bauer TW, Muschler GF: Bone graft materials. An overview of the basic science. Clin Orthop Relat Res. 2000, 10-27. 10.1097/00003086-200002000-00003.Google Scholar
- Burstein FD: Bone substitutes. Cleft Palate Craniofac J. 2000, 37: 1-4. 10.1597/1545-1569(2000)037<0001:ESNTIT>2.3.CO;2.View ArticlePubMedGoogle Scholar
- Delloye C, Cnockaert N, Cornu O: Bone substitutes in 2003: an overview. Acta Orthop Belg. 2003, 69: 1-8.PubMedGoogle Scholar
- Hollinger JO, Brekke J, Gruskin E, Lee D: Role of bone substitutes. Clin Orthop Relat Res. 1996, 55-65. 10.1097/00003086-199603000-00008.Google Scholar
- Van Heest A, Swiontkowski M: Bone-graft substitutes. Lancet. 1999, 353 (Suppl 1): SI28-SI29.View ArticlePubMedGoogle Scholar
- Pietrzak WS, Ronk R: Calcium sulfate bone void filler: a review and a look ahead. J Craniofac Surg. 2000, 11: 327-333. 10.1097/00001665-200011040-00009.View ArticlePubMedGoogle Scholar
- Schnurer SM, Gopp U, Kuhn KD, Breusch SJ: [Bone substitutes]. Orthopade. 2003, 32: 2-10. 10.1007/s00132-002-0407-9.View ArticlePubMedGoogle Scholar
- Briem D, Linhart W, Lehmann W, Meenen NM, Rueger JM: [Long-term outcomes after using porous hydroxyapatite ceramics (Endobon) for surgical management of fractures of the head of the tibia]. Unfallchirurg. 2002, 105: 128-133. 10.1007/s001130100303.View ArticlePubMedGoogle Scholar
- Mahan KT, Carey MJ: Hydroxyapatite as a bone substitute. J Am Podiatr Med Assoc. 1999, 89: 392-397.View ArticlePubMedGoogle Scholar
- Cassidy C, Jupiter JB, Cohen M, li-Santi M, Fennell C, Leinberry C: Norian SRS cement compared with conventional fixation in distal radial fractures. A randomized study. J Bone Joint Surg Am. 2003, 85-A: 2127-2137.PubMedGoogle Scholar
- Sarkar MR, Wachter N, Patka P, Kinzl L: First histological observations on the incorporation of a novel calcium phosphate bone substitute material in human cancellous bone. J Biomed Mater Res. 2001, 58 (3): 329-334. 10.1002/1097-4636(2001)58:3<329::AID-JBM1025>3.0.CO;2-9.View ArticlePubMedGoogle Scholar
- Trenholm A, Landry S, McLaughlin K, Deluzio KJ, Leighton J, Trask K: Comparative fixation of tibial plateau fractures using alpha-BSM, a calcium phosphate cement, versus cancellous bone graft. J Orthop Trauma. 2005, 19 (10): 698-702. 10.1097/01.bot.0000183455.01491.bb.View ArticlePubMedGoogle Scholar
- Simpson D, Keating JF: Outcome of tibial plateau fractures managed with calcium phosphate cement. Injury. 2004, 35: 913-918. 10.1016/S0020-1383(03)00109-8.View ArticlePubMedGoogle Scholar
- Gierse H, Donath K: Reactions and complications after the implantation of Endobon including morphological examination of explants. Arch Orthop Trauma Surg. 1999, 119: 349-355. 10.1007/s004020050426.View ArticlePubMedGoogle Scholar
- Hing KA, Best SM, Tanner KE, Bonfield W, Revell PA: Mediation of bone ingrowth in porous hydroxyapatite bone graft substitutes. J Biomed Mater Res A. 2004, 68: 187-200. 10.1002/jbm.a.10050.View ArticlePubMedGoogle Scholar
- Jarcho M: Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop Relat Res. 1981, 259-278.Google Scholar
- Fernandez E, Gil FJ, Ginebra MP, Driessens FC, Planell JA, Best SM: Calcium phosphate bone cements for clinical applications. Part II: precipitate formation during setting reactions. J Mater Sci Mater Med. 1999, 10: 177-183. 10.1023/A:1008989525461.View ArticlePubMedGoogle Scholar
- Fernandez E, Gil FJ, Ginebra MP, Driessens FC, Planell JA, Best SM: Calcium phosphate bone cements for clinical applications. Part I: solution chemistry. J Mater Sci Mater Med. 1999, 10: 169-176. 10.1023/A:1008937507714.View ArticlePubMedGoogle Scholar
- Lobenhoffer P, Gerich T, Witte F, Tscherne H: Use of an injectable calcium phosphate bone cement in the treatment of tibial plateau fractures: a prospective study of twenty-six cases with twenty-month mean follow-up. J Orthop Trauma. 2002, 16: 143-149. 10.1097/00005131-200203000-00001.View ArticlePubMedGoogle Scholar
- Keating JF, Hajducka CL, Harper J: Minimal internal fixation and calcium-phosphate cement in the treatment of fractures of the tibial plateau. A pilot study. J Bone Joint Surg Br. 2003, 85: 68-73. 10.1302/0301-620X.85B1.12575.View ArticlePubMedGoogle Scholar
- Gangopadhyay S, Ravi K, Packer G: Dorsal plating of unstable distal radius fractures using a bio-absorbable plating system and bone substitute. J Hand Surg [Br]. 2006, 31 (1): 93-100.View ArticleGoogle Scholar
- Bajammal SS, Zlowodzki M, Lelwica A, Tornetta P, Einhorn TA, Buckley R: The use of calcium phosphate bone cement in fracture treatment. A meta-analysis of randomized trials. J Bone Joint Surg Am. 2008, 90 (6): 1186-1196. 10.2106/JBJS.G.00241.View ArticlePubMedGoogle Scholar
- Welch RD, Zhang H, Bronson DG: Experimental tibial plateau fractures augmented with calcium phosphate cement or autologous bone graft. J Bone Joint Surg Am. 2003, 85-A (2): 222-231.PubMedGoogle Scholar
- Kopylov P, Adalberth K, Jonsson K, Aspenberg P: Norian SRS versus functional treatment in redisplaced distal radial fractures: a randomized study in 20 patients. J Hand Surg [Br]. 2002, 27: 538-541.View ArticleGoogle Scholar
- Sanchez-Sotelo J, Munuera L, Madero R: Treatment of fractures of the distal radius with a remodellable bone cement: a prospective, randomised study using Norian SRS. J Bone Joint Surg Br. 2000, 82: 856-863. 10.1302/0301-620X.82B6.10317.View ArticlePubMedGoogle Scholar
- Dickson KF, Friedman J, Buchholz JG, Flandry FD: The use of BoneSource hydroxyapatite cement for traumatic metaphyseal bone void filling. J Trauma. 2002, 53: 1103-1108. 10.1097/00005373-200212000-00012.View ArticlePubMedGoogle Scholar
- Zimmermann R, Gabl M, Lutz M, Angermann P, Gschwentner M, Pechlaner S: Injectable calcium phosphate bone cement Norian SRS for the treatment of intra-articular compression fractures of the distal radius in osteoporotic women. Arch Orthop Trauma Surg. 2003, 123: 22-27.PubMedGoogle Scholar
- Russell TA, Leighton RK: Comparison of autogenous bone graft and endothermic calcium phosphate cement for defect augmentation in tibial plateau fractures. A multicenter, prospective, randomized study. J Bone Joint Surg Am. 2008, 90 (10): 2057-2061. 10.2106/JBJS.G.01191.View ArticlePubMedGoogle Scholar
- Huber FX, Hillmeier J, Herzog L, McAthur N, Kock HJ, Meeder PJ: Open reduction and palmar plate-osteosynthesis in combination with a nanocrystalline hydroxyapatite spacer in the treatment of comminuted fractures of the distal radius. J Hand Surg [Br]. 2006, 31 (3): 298-303.View ArticleGoogle Scholar
- Huber FX, McArthur N, Hillmeier J, Kock HJ, Baier M, Diwo M: Void filling of tibia compression fracture zones using a novel resorbable nanocrystalline hydroxyapatite paste in combination with a hydroxyapatite ceramic core: first clinical results. Arch Orthop Trauma Surg. 2006, 126: 533-540. 10.1007/s00402-006-0170-1.View ArticlePubMedGoogle Scholar
- Huber FX, Hillmeier J, McArthur N, Kock HJ, Meeder PJ: The use of nanocrystalline hydroxyapatite for the reconstruction of calcaneal fractures: Preliminary results. J Foot Ankle Surg. 2006, 45: 322-328. 10.1053/j.jfas.2006.05.006.View ArticlePubMedGoogle Scholar
- Bezrukov VM, Grigor'iants LA, Zuev VP, Pankratov AS: [The surgical treatment of jaw cysts using hydroxyapatite with an ultrahigh degree of dispersity]. Stomatologiia (Mosk). 1998, 77: 31-35.Google Scholar
- Zuev VP, Dmitrieva LA, Pankratov AS, Filatova NA: [The comparative characteristics of stimulators of reparative osteogenesis in the treatment of periodontal diseases]. Stomatologiia (Mosk). 1996, 75: 31-34.Google Scholar
- Schwarz F, Bieling K, Latz T, Nuesry E, Becker J: Healing of intrabony peri-implantitis defects following application of a nanocrystalline hydroxyapatite (Ostim) or a bovine-derived xenograft (Bio-Oss) in combination with a collagen membrane (Bio-Gide). A case series. J Clin Periodontol. 2006, 33: 491-499. 10.1111/j.1600-051X.2006.00936.x.View ArticlePubMedGoogle Scholar
- Strietzel FP, Reichart PA, Graf HL: Lateral alveolar ridge augmentation using a synthetic nano-crystalline hydroxyapatite bone substitution material (Ostim). Preliminary clinical and histological results. Clin Oral Implants Res. 2007, 18: 743-751. 10.1111/j.1600-0501.2007.01416.x.View ArticlePubMedGoogle Scholar
- Rauschmann MA, Wichelhaus TA, Stirnal V, Dingeldein E, Zichner L, Schnettler R: Nanocrystalline hydroxyapatite and calcium sulphate as biodegradable composite carrier material for local delivery of antibiotics in bone infections. Biomaterials. 2005, 26: 2677-2684. 10.1016/j.biomaterials.2004.06.045.View ArticlePubMedGoogle Scholar
- Aebli N, Stich H, Schawalder P, Theis JC, Krebs J: Effects of bone morphogenetic protein-2 and hyaluronic acid on the osseointegration of hydroxyapatite-coated implants: an experimental study in sheep. J Biomed Mater Res A. 2005, 73 (3): 295-302.View ArticlePubMedGoogle Scholar
- Cimerman M, Cor A, Ceh M, Kristan A, Pizem J, Tonin M: Microstructural analysis of implant-bone interface of hydroxyapatite-coated and uncoated Schanz screws. J Mater Sci Mater Med. 2005, 16 (7): 627-634. 10.1007/s10856-005-2533-5.View ArticlePubMedGoogle Scholar
- Donath K, Breuner G: A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique. J Oral Pathol. 1982, 11: 318-326. 10.1111/j.1600-0714.1982.tb00172.x.View ArticlePubMedGoogle Scholar
- Draenert K, Wiese FG, Garde U, Draenert Y, Helber U, Börner M: Synthetic bone substitutes based on hydroxyapatites and tricalcium phosphates. Trauma und Berufskrankheiten. 2001, 3: 293-300. 10.1007/s10039-001-0494-6.View ArticleGoogle Scholar
- Knaack D, Goad ME, Aiolova M, Rey C, Tofighi A, Chakravarthy P: Resorbable calcium phosphate bone substitute. J Biomed Mater Res. 1998, 43: 399-409. 10.1002/(SICI)1097-4636(199824)43:4<399::AID-JBM7>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Noetzel J, Kielbassa AM: [Calcium phosphate cements in medicine and dentistry--a review of literature]. Schweiz Monatsschr Zahnmed. 2005, 115 (12): 1148-1156.PubMedGoogle Scholar
- Giannoudis PV, Dinopoulos H, Tsiridis E: Bone substitutes: an update. Injury. 2005, 36 (Suppl 3): S20-S27. 10.1016/j.injury.2005.07.029.View ArticlePubMedGoogle Scholar
- Welch RD, Berry BH, Crawford K, Zhang H, Zobitz M, Bronson D: Subchondral defects in caprine femora augmented with in situ setting hydroxyapatite cement, polymethylmethacrylate, or autogenous bone graft: biomechanical and histomorphological analysis after two-years. J Orthop Res. 2002, 20 (3): 464-472. 10.1016/S0736-0266(01)00124-3.View ArticlePubMedGoogle Scholar
- Frankenburg EP, Goldstein SA, Bauer TW, Harris SA, Poser RD: Biomechanical and histological evaluation of a calcium phosphate cement. J Bone Joint Surg Am. 1998, 80 (8): 1112-1124.PubMedGoogle Scholar
- Constantz BR, Ison IC, Fulmer MT, Poser RD, Smith ST, VanWagoner M: Skeletal repair by in situ formation of the mineral phase of bone. Science. 1995, 267: 1796-1799. 10.1126/science.7892603.View ArticlePubMedGoogle Scholar
- Posner AS: The mineral of bone. Clin Orthop Relat Res. 1985, 87-99.Google Scholar
- Kilian O, Alt V, Heiss C, Jonuleit T, Dingeldein E, Flesch I: New blood vessel formation and expression of VEGF receptors after implantation of platelet growth factor-enriched biodegradable nanocrystalline hydroxyapatite. Growth Factors. 2005, 23: 125-133. 10.1080/08977190500126306.View ArticlePubMedGoogle Scholar
- Blokhuis TJ, Termaat MF, den Boer FC, Patka P, Bakker FC, Haarman HJ: Properties of calcium phosphate ceramics in relation to their in vivo behavior. J Trauma. 2000, 48: 179-186. 10.1097/00005373-200001000-00037.View ArticlePubMedGoogle Scholar
- Grigor'ian AS, Grigor'iants LA, Podoinikova MN: [A comparative analysis of the efficacy of different types of filling materials in the surgical elimination of tooth perforations (experimental morphological research)]. Stomatologiia (Mosk). 2000, 79: 9-12.Google Scholar
- Huber FX, Berger I, McArthur N, Huber C, Kock HP, Hillmeier J: Evaluation of a novel nanocrystalline hydroxyapatite paste and a solid hydroxyapatite ceramic for the treatment of critical size bone defects (CSD) in rabbits. J Mater Sci Mater Med. 2008, 19 (1): 33-38. 10.1007/s10856-007-3039-0.View ArticlePubMedGoogle Scholar
- Laschke MW, Witt K, Pohlemann T, Menger MD: Injectable nanocrystalline hydroxyapatite paste for bone substitution: in vivo analysis of biocompatibility and vascularization. J Biomed Mater Res B Appl Biomater. 2007, 82: 494-505.View ArticlePubMedGoogle Scholar
- Spies C, Schnurer S, Gotterbarm T, Breusch S: [Animal study of the bone substitute material Ostim within osseous defects in Gottinger minipigs]. Z Orthop Unfall. 2008, 146 (1): 64-69. 10.1055/s-2007-965756.View ArticlePubMedGoogle Scholar
- Gerlach KL, Niehues D: Treatment of jaw cysts with a new kind of nanoparticular hydroxylapatite. Mund Kiefer Gesichtschir. 2007, 11: 131-137. 10.1007/s10006-007-0064-6.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/10/164/prepub
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