Fig. 4
From: FTH1 protects against osteoarthritis by MAPK pathway inhibition of extracellular matrix degradation
Overexpress FTH1 reduces cartilage loss and protect chondrocytes from DMM surgery. A Safranin O and Fast Green staining and quantification of sham-operated mice and mice treated with vehicle and Ad-FTH1 for 4 weeks after DMM surgery. n = 5 per group; Scale bar: 100 µm; B Osteoarthritis Research Society International (OARSI) grades of the joints described in A. n = 5 per group; C-D H&E staining of sham-operated mice and mice treated with vehicle and Ad-FTH1 for 4 weeks after DMM surgery; Scale bar: 200 µm; Immunohistochemical staining and quantification of FTH1 (E, F), GPX4 (G,H), SLC7A11 (I, J) aggrecan (L, L) and SOX9 (M, N); n = 5 per group; *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001; Scale bars: 200 µm (first row) and 100 µm (second row)