Table 1 List of genes and their specific primer sequences for RT-PCR validation
Gene name | Primer | Sequence |
|---|---|---|
Aggrecan | Forward primer | 5'-GGACTTCCGCTGGTCAGATG-3' |
| Â | Reverse primer | 5'-GTTTGTAGGTGGTGGCTGTG-3' |
CollagenII | Forward primer | 5'- CCGTGCTCCTGCCGTTTC-3' |
| Â | Reverse primer | 5'- CTGAGGCAGTCTTTCACGTCT-3' |
Sox9 | Forward primer | 5'- TCTGAACGAGAGCGAGAAGC-3' |
| Â | Reverse primer | 5'- CCGTTCTTCACCGACTTCCT-3' |
GAPDH | Forward primer | 5'- AATGGGCAGCCGTTAGGAAA-3' |
| Â | Reverse primer | 5'- GCGCCCAATACGACCAAATC-3' |