Fig. 5

Induced chondrogenic properties assay. A I- III Alizarin red staining, type II collagen staining, and AB-PSA staining to assess chondrogenic properties, respectively; (B) Western blot to detect the protein expression of Aggrecan, Sox9, and Collagen II; (C) Relative expression of Aggrecan, Sox9, and Collagen II proteins; (D) RT-PCR to detect the relative expression of Aggrecan, Sox9, and Collagen II mRNA