Table 1 Primers used for RT-PCR analysis
From: Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression
Gene | Forward primer | Reverse primer |
|---|---|---|
circ_0027885 | 5’-AGCCATCTGTGTTGCTGTTG‐3’ | 5’-ACAAATAAGTTTCCTTGGATACACC‐3’ |
RUNX2 | 5’-CCGGTCTCCTTCCAGAAT‐3’ | 5’-GGGAACTGCTGTGGCTTC‐3’ |
β-actin | 5’-CCATGTACGTTGCTATCCAG‐3’ | 5’-CTTCATGAGGTAGTCAGTCAG‐3’ |
miR-203-3p | 5’-ATGGTTCGTGGGGTGAAATGTTTAGGAC‐3’ | 5’-GCAGGGTCCGAGGTATTC‐3’ |
U6 | 5’-CTCGCTTCGGCAGCACA‐3’ | 5’-AACGCTTCACGAATTTGCGT‐3’ |