Fig. 3
From: Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression
MiR-203‐3p was sponged by circ_0027885. (A) The binding sites between miR‐203‐3p and circ_0027885, predicted by bioinformatics analysis. (B) Circ_0027885-wt and circ_0027885-mut luciferase activity in 293T cells treated with miR‐203‐3p mimic or NC mimic, determined by dual‐luciferase reporter gene assay. *P < 0.05, as comparison to the NC mimic group. The luciferase activity of circ_0027885-wt with NC mimic group was normalized. (C) Relative expression of miR-203-3p in hBMSCs after infection with LV-circ_0027885 or sh-circ_0027885, validated by qRT-PCR. **P < 0.01, as comparison to the LV-NC group, **P < 0.01, as comparison to the sh-NC group. The miR-203-3p expression of LV-NC group was normalized. (D) Relative expression of miR‐203‐3p in hBMSCs treated with osteogenic differentiation medium for 0, 1, 3, 7, and 14 days, detected by RT-qPCR. **P < 0.01, ***P < 0.001, as comparison to the 0 day group. The miR-203-3p expression of 0 day group was normalized