Fig. 2
From: Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression
Circ_0027885 promoted osteogenic differentiation of hBMSCs. (A-B) Transfection efficiency after infecting LV-circ_0027885 or sh-circ_0027885, validated by qRT-PCR. ****P < 0.0001, as comparison to the LV-NC group, *P < 0.05, as comparison to the sh-NC group. The circ_0027885 expression of LV-NC or sh-NC group was normalized. (C-D) Relative protein expression of RUNX2 in hBMSCs after infection with LV-circ_0027885 or sh-circ_0027885, measured by western blot. **P < 0.01, as comparison to the LV-NC group, **P < 0.01, as comparison to the sh-NC group. The RUNX2 expression of LV-NC group was normalized. (E) Relative ALP activity was examined in hBMSCs after infection with LV-circ_0027885 or sh-circ_0027885. **P < 0.01, as comparison to the LV-NC group, **P < 0.01, as comparison to sh-NC group. The ALP activity of LV-NC group was normalized. (F) The mineralization levels of hBMSCs after infection with LV-circ_0027885 or sh-circ_0027885, as determined by Alizarin red staining