Fig. 3
From: Upregulating miR-181b promotes ferroptosis in osteoarthritic chondrocytes by inhibiting SLC7A11
miR-181b mediates ferroptosis in chondrocytes by targeting SLC7A11. (A) qPCR detection of SLC7A11 gene expression. (B) hsa-miR-181b-5p and SLC7A11 target binding sequence and mutation sequence. (C) Dual luciferase activity reporter detection of the targeting relationship between hsa-miR-181b-5p and SLC7A11. (D) qPCR detection of SLC7A11 siRNA-mediated inhibition of SLC7A11 gene. (E) The inhibition efficiency of SLC7A11 siRNA on SLC7A11 protein expression was detected by Western blotting. (F) Statistical quantification of the optical density values of the Western blot bands. (G) CCK-8 assays to detect the effect of the miR-181b inhibitor or si-SLC7A11 on chondrocyte viability. (H) Immunofluorescence analysis to detect the expression level and distribution of SLC7A11. (I) Immunofluorescence analysis to quantify the fluorescence intensity. (J-K) Western blot analysis of the expression levels of ferroptosis-related proteins. **P < 0.01, *P < 0.05, compared with the control group, Erastin group: treated with 1 µM erastin, miR-181b group: treated with 1 µM erastin + miR-181b inhibitor, si-SLC7A11 group: treated with 1 µM erastin + si-SLC7A11. ##P < 0.01, #P < 0.05, the difference between the two groups is statistically significant