Fig. 2
From: Upregulating miR-181b promotes ferroptosis in osteoarthritic chondrocytes by inhibiting SLC7A11
Inhibition of miR-181b inhibited the occurrence of ferroptosis. (A-B) qPCR detection of miR-181b gene expression levels. (C) Western blot detection of matrix-related protein and ferroptosis-related protein expression levels. (D-F) Western blot detection of bands and statistical quantification of the density values. (G) detection of MDA levels in cells. (H) Detection of GSH levels in cells. (I) ROS levels were detected by flow cytometry. (J) Detection of Fe2+ levels in cells. (K) Ferrous ions were stained with the Ferro Orange fluorescent probe. **P < 0.01, *P < 0.05, the difference between the model group and the control group was statistically significant. G-K. Erastin group: treated with 1 µM erastin, miR-181b group: treated with 1 µM erastin + miR-181b inhibitor. ##P < 0.01, #P < 0.05, the difference between the miR-181b in the group and the model group was statistically significant