Fig. 1
From: Upregulating miR-181b promotes ferroptosis in osteoarthritic chondrocytes by inhibiting SLC7A11
Erastin-induced ferroptosis in chondrocytes. (A) Immunofluorescence was used to detect the protein expression of aggrecan and collagen II. (B-C) CCK-8 detection of cell activity. (D) Alcian blue staining of chondrocyte matrix. (E-F) Western blot detection of matrix-related proteins (collagen II, ACNA and MMP13) and ferroptosis-related proteins (TFR1, FTH1, p53, SLC7A11, and GPX4) expression level. (G-I) Western blot optical density value quantification. (J) Cell MDA level detection. (K) Cell GSH level detection. (L) Flow cytometry analysis of cellular ROS accumulation and fluorescent labeling with DCFH-Da. (M) Detection of Fe2+ levels in cells. **P < 0.01, *P < 0.05, the difference between the model group and the control group was statistically significant. ##P < 0.01, #P < 0.05, the difference between the erastin + Fer group and model group was statistically significant