Fig. 6

Fluorescent staining of the callus after green fluorescent protein (GFP) bone marrow transplantation. Double image of TNF-α and GFP. The upper column shows samples without long-term hyperglycaemia (LT-HG) and the lower column shows samples with LT-HG. TNF-α-positive cells are stained blue, and bone marrow-derived cells are stained green. The white arrow in the right side panel shows the double image of TNF-α and GFP. (bar: 100 μm). The graph shows the proportion of GFP-positive cells (GFP+) and TNF-α-positive cells. N.S: no significant difference. GFP and LT-HG positive; n = 5, GFP positive and LT-HG negative; n = 5