Fig. 5

Fluorescent staining of the callus after green fluorescent protein (GFP) bone marrow transplantation. Double image of proinsulin and GFP staining. The upper column shows samples without long-term hyperglycemia (LT-HG) and the lower column shows samples with LT-HG. Proinsulin-positive cells are stained red and bone marrow-derived cells are stained green. The white arrow in the right side panel shows a double image of proinsulin and GFP. (bar: 100 μm). The graph shows the proportion of GFP-positive cells (GFP+) and proinsulin-positive cells. *: p < 0.05. GFP and LT-HG positive; n = 5, GFP positive and LT-HG negative; n = 5