Skip to main content
Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: Astragalus polysaccharide ameliorates steroid-induced osteonecrosis of the femoral head by regulating miR-200b-3p-mediated Wnt/β-catenin signaling pathway via inhibiting SP1 expression

Fig. 5

Silencing miR-200b-3p weakened effect of SP1 overexpression on SONFH cell model by activating Wnt/β-catenin signaling. APS-treated SONFH cell model was transfected with oe-SP1 and miR-200b-3p inhibitor (APS + oe-SP1 + miR-200b-3p inhibitor group) or inhibitor NC (APS + oe-SP1 + inhibitor NC group). (A) RT-qPCR assessed miR-200b-3p expression. (B) Western blot measured β-catenin protein level. (C) Immunofluorescence detection of LC3 expression in cells. (D) Western blot determination of autophagy-related proteins. (E) Flow cytometry analyzed cell apoptosis. (F) Western blot analysis of apoptosis-related proteins. Results expressed as mean ± SD. *P < 0.05, **P < 0.01

Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us