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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Astragalus polysaccharide ameliorates steroid-induced osteonecrosis of the femoral head by regulating miR-200b-3p-mediated Wnt/β-catenin signaling pathway via inhibiting SP1 expression

Fig. 4

SP1 inhibited Wnt/β-catenin signaling by targeting miR-200b-3p. (A) ALGGEN predicted binding sequence of SP1 to miR-200b-3p promoter. (B) Dual luciferase reporter assay verified targeting relationship between SP1 and miR-200b-3p. (C) ChIP assay detected effect of SP1 on miR-200b-3p promoter. APS-treated SONFH cell model was then transfected with oe-SP1 or control vector. (D) RT-qPCR assessed miR-200b-3p expression. (E) Western blot measured β-catenin protein level. Values are mean ± SD. **P < 0.01

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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