Skip to main content
Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Astragalus polysaccharide ameliorates steroid-induced osteonecrosis of the femoral head by regulating miR-200b-3p-mediated Wnt/β-catenin signaling pathway via inhibiting SP1 expression

Fig. 1

APS induced autophagy and reduced apoptosis in DEX-treated MLO-Y4 cells. MLO-Y4 cells were exposed to the treatment with 10− 5 M dexamethasone (DEX) for 3 days with the purpose of constructing SONFH cell model. (A) MTT assay examined the effect of different concentrations of APS on the viability of MLO-Y4 cells. Then, cells were subject to treatment with 8 mg/ml APS for one day. (B) Immunofluorescence detection of LC3 expression. (C) Western blot determination of autophagy-related proteins (LC3II/LC3I, Beclin1, p62). (D) Flow cytometry analyzed cell apoptosis. (E) Western blot analyzed apoptosis-related proteins (Bax, C-caspase3, C-caspase9, Bcl-2). Results expressed as mean ± SD. **P < 0.01,***P < 0.001

Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us