Fig. 3
From: Nutritional deficiency induces nucleus pulposus cell apoptosis via the ATF4-PKM2-AKT signal axis
ATF4 silence can increase NP cell’s glucose uptake and lactic acid level under the nutritional deficiency condition
(A) The NP cell’s glucose uptake level under the condition of nutritional deficiency and ATF4 silence (Group A, B, C denotes the 10%FBS, 1%FBS and 1%FBS with siATF4 group respectively) (B) The NP cell’s lactic acid level under the condition of nutritional deficiency and ATF4 silence (Group A, B, C denotes the 10%FBS, 1%FBS and 1%FBS with siATF4 group respectively) (C) The glucolysis status detected by western blot under the condition of nutritional deficiency and ATF4 silence (Group A, B, C denotes the 10%FBS, 1%FBS and 1%FBS with siATF4 group respectively) (D) The quantity the protein reflected glucolysis status as showed in C. Data are mean ± s.d. The percentage calculation in each tissue were measured at least 1000 cells each sample. Statistical significance was determined by one-way ANOVA and Student’s t-test. **, ##, △△ P < .001