Fig. 1
From: Nutritional deficiency induces nucleus pulposus cell apoptosis via the ATF4-PKM2-AKT signal axis
Nutritional deficiency can inhibit increase the expression of ATF4 and inhibit PKM2 in NP cells
(A) The NP cell proliferation status detected by CCK8 under the different condition of nutritional deficiency (B)The expression level of ATF4 and PKM2 detected by western blot under the different condition of nutritional deficiency. (Group A, B, C, D denotes the media contain 10%, 5%, 1% and 0% FBS, respectively) (C) The quantity analysis of expression level of ATF4 and PKM2 as showed in B (D) The expression level of ATF4 and PKM2 detected by western blot under serum free condition with different time (E) The quantity analysis of expression level of ATF4 and PKM2 as showed in D. Data are mean ± s.d. The percentage calculation in each tissue were measured at least 1000 cells each sample. Statistical significance was determined by one-way ANOVA and Student’s t-test. **, ##, △△ P < .001