Table 1 Details of primer pairs used

MMP1

5'-CTG GCC ACA ACT GCC AAA TG-3'

5'-CTG TCC CTG AAC AGC CCA GTA CTT A-3'

MMP2

5'-TGT TTG TGC TGA AGG ACA CAC TAA-3'

5'-CTT GCG AGG GAA GAA GTT GTA GTT-3'

MMP3

5'-TCA TTT TGG CCA TCT CTT CC-3'

5'-TGG CTC CAG GAA TTT CTC T-3'

MMP9

5'-AAG GCG CAG ATG GTG GAT-3'

5'-GCA GGA TGT CAT AGG TCA CGT A-3'

MMP13

5'-TAA GGA GCA TGG CAC TTC T-3'

5'-GTC TGG CGT TTT TGG ATG TT-3

TIMP1

5'-GCT GGA AAA CTG CAG GAT GGACTC-3'

5'-CTG GAA GCC CTT TTC AGA GCC TTG-3'

TIMP2

5'-CTT AGT GTT CCC TCC CTC AAA GAC-3'

5'-AAG AAG TGA GTG TGT CAC CAA AGC-3'

TIMP3

5'-AGC TTC CGA GAG TCT CTG TG-3'

5'-GTA GCA GGA CTT GAT CTT GCA GTT-3'

IL1B

5'-TCG CCA GTG AAA TGA TGG CTT-3'

5'-GTC CAT GGC CAC AAC AAC TGA-3'

IL6

5'-ACT CAC CTC TTC AGA ACG AAT TG-3'

5'-CCA TCT TTG GAA GGT TCA GGT TG-3'

TNF

5'-CTC TCT CCC CTG GAA AGG AC-3'

5'-TCA CCC ATC CCA TCT CTC TC-3'

IGF1

5'-ACA TCT CCC ATC TCT CTG GAT TTC CTT TTG C-3’

5'-CCC TCT ACT TGC GTT CTT CAA ATG TAC TTC C-3'

TGFB1

5'-AGG ACC TCG GCT GGA AGT GGA T-3'

5'-AGG CGC CCG GGT TAT GCT-3'

COL1A1

5'-AGC CTG GGG CAA GAC ATG ATT-3'

5'-TTG CTT GTC TGT TTC CGG GTT G-3'

COL2A1

5'-AAT TCC TGG AGC CAA AGG AT-3'

5'-AGG ACC AGT TGC ACC TTG AG-3'

SOX9

5'-AGC GAA CGC ACA TCA AGA C-3'

5'-CTG TAG GCG ATC TGT TGG GG-3'

NGF

5'-GGC AGA CCC GCA ACA TTA CT-3'

5'-CAC CAC CGA CCT CGA AGT C-3'

NGFR

5'-TGG CTC CCC TCT ATT TAG CAT G-3'

5'-ATA CTT GCA AGC CCC CAA AC-3'

ACTA2

5'-GAG ATC TCA CTG ACT ACC TCA TGA-3'

5'-AGA GCT ACA TAA CAC AGT TTC TCC TTG A-3'