Fig. 2
From: miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP
Impacts of miR-215-5p on hBMSCs osteogenic differentiation. A Western blot assay was conducted to detect ALP, OCN and RUNX2 expressions. B ALP activity was examined to evaluate the mineralization of hMBSCs. C Alizarin red staining was used to measure calcium deposits in hBMSCs. D Expression of miR-215-5p after β-glycerophosphate treatment in hBMSCs. E Transfection efficacy of miR-215-5p was verified by qRT-PCR analysis. F Effect of miR-215-5p transfection on ALP, OCN and RUNX2 was confirmed by western blot assay. G Effect of miR-215-5p on ALP activity in hBMSCs. H Effect of miR-215-5p on Alizarin red staining result in hBMSCs