Fig. 5

Immunohistochemistry staining of PPARγ protein in primary bone tumors. The hematoxylin and eosin (H&E) staining (a-c) and immunohistochemistry of PPARγ (d-f) in bone tumor tissues were assessed. The representative images of H&E staining of the osteosarcoma tumor tissue (a), Ewing sarcoma (b) and GCT (c) are shown. The negative immune-reactivity of PPARγ is shown in (d), the weak intensity of PPARγ (< 10% immune-reactivity) is shown in (e) and the strong intensity of PPARγ (> 20% immune-reactivity) is shown in (f). The cytoplasmic expression of PPARγ is shown in (e) and (f) that represents Ewing sarcoma and osteosarcoma tumor tissues, respectively. The separated immunohistochemistry images into staining for the nuclei and DAB-positive area is shown (g-l). The staining for the nuclei is illustrated in g-i as (g): negative staining, (h): weak intensity, and (i): strong intensity. The DAB staining for target PPARγ protein expression is shown in j-l as (j): negative intensity, (k): weak intensity, and (l): strong intensity. The analysis was followed the Crowe’s method [22]. The scale of magnification for (a-c) is 40 and for (d-l) is 200