Table 1 Primer pairs used for quantitative PCR analysis
Gene name | Forward primer | Reverse primer |
|---|---|---|
TNF | 5′AAGGACACCATGAGCACTGAAAGC3′ | 5′AGGAAGGAGAAGAGGCTGAGGAAC3′ |
IL1-β | 5′GCCAGTGAAATGATGGCTTATT3′ | 5′AGGAGCACTTCATCTGTTTAGG3′ |
IL-6 | 5′CACTGGTCTTTTGGAGTTTGAG3′ | 5′GGACTTTTGTACTCATCTGCAC3′ |
IL-8 | 5′AACTGAGAGTGATTGAGAGTGG3′ | 5′ATGAATTCTCAGCCCTCTTCAA3′ |
NOS2 | 5′ AGGGACAAGCCTACCCCTC 3′ | 5′ CTCATCTCCCGTCAGTTGGT3′ |
TGF-β | 5′CTGTACATTGACTTCCGCAAG 3′ | 5′ TGTCCAGGCTCCAAATGTAG3′ |
GAPDH | 5′CTTAGCACCCCTGGCCAAG3′ | 5′TGGTCATGAGTCCTTCCACG3′ |