Fig. 3
From: Impaired expression of BCAT1 relates to muscle atrophy of mouse model of sarcopenia
Bcat1 knockdown impairs cell growth and promotes ROS production in muscle cells. A ROS concentration in C2C12 cells was detected by the fluorescence method. Grouping: control vector (shCtrl), shBcat1, shBcat1 + mTOR activator, shBcat1 + shRNA-resistant Bcat1 cDNA (rescue), shBcat1 + shRNA-resistant Bcat1 cDNA (rescue) + mTOR inhibitor, n = 3, cells were analyzed at 24 h post-treatment. B Viability of C2C12 cells was detected by the CCK8 method. Grouping: control vector (shCtrl), shBcat1, shBcat1 + mTOR activator, shBcat1 + shRNA-resistant Bcat1 cDNA (rescue), shBcat1 + shRNA-resistant Bcat1 cDNA (rescue) + mTOR inhibitor, n = 3, cells were analyzed at 24 h, 48 h, 72 h post-treatment. Data from three independent experiments are presented as mean + SEM. ***p < 0.001