Fig. 5

Knockdown of PAR-2 alleviated CIBP by inhibiting the activation of the ERK pathway and inhibiting the activation of astrocytes. The L4-5 spinal cord tissues of TCI rats on the 14th day after injection of PAR-2 inhibitor and ERK activator were obtained. A The levels of the ERK pathway-related proteins were detected by WB; B The mRNA level of GFAP was detected by RT-qPCR; C The protein level of GFAP was detected by WB; D The changes of mechanical pain threshold and thermal pain threshold were detected on the 3rd, 5th, 7th and 14th day. N = 6. The data were expressed as mean ± standard deviation. Independent t test was used for comparisons of data between 2 groups. *P < 0.05, **P < 0.01, ***P < 0.001. t-CREB: total CREB; t-ERK: total ERK