Fig. 3

Knockdown of PAR-2 inhibited the activation of GFAP to alleviate CIPB in rats. The L4-5 spinal cord was obtained on the 14th day after modeling. A The expression of PAR-2 was detected by WB; B The mRNA level of astrocyte marker GFAP was detected by RT-qPCR; C The protein level of GFAP was detected by WB; D The changes of mechanical pain threshold and thermal pain threshold were detected on the 3rd, 5th, 7th and 14th day. N = 6. The data were expressed as mean ± standard deviation. One-way ANOVA was used for comparisons of data among multi-groups. Tukey’s multiple comparisons test was used for post hoc test *P < 0.05, **P < 0.01