Fig. 2

The expression of PAR-2 in the spinal cord of TCI rats was upregulated. On the 3rd, 5th, 7th, 14th and 21st day after modeling, the L4-5 spinal cord tissues were obtained. A The expression of PAR-2 in the spinal cord of rats was detected by WB; B On the 14th day, the co-expression of PAR-2 and spinal astrocytes was detected by immunofluorescence double staining (the scale was 25 μm); C The mRNA level of astrocyte marker GFAP in rat spinal cord was observed by RT-qPCR; D The astrocyte marker GFAP protein level in rat spinal cord was detected by WB. N = 6. The data were expressed as mean ± standard deviation. One-way ANOVA was used for comparisons of data in panel A-D. Tukey’s multiple comparisons test was used for post hoc test *Compared with the sham group, P < 0.05, **Compared with the sham group, P < 0.01