Fig. 4
From: Antioxidant effect of nicotinamide mononucleotide in tendinopathy
ROS accumulation in vitro. Analysis of ROS accumulation in four different in vitro conditions: regular-glucose without NMN (RG NMN(−)), regular-glucose with NMN (RG NMN(+)), high-glucose without NMN (HG NMN(−)), and high-glucose with NMN (HG NMN(+)). (a) Fluorescence staining showing ROS accumulation (green; white arrow) and DAPI staining (blue) in tenocytes. (b) Quantification of intracellular ROS levels in the four groups. ROS accumulation was significantly higher in the HG NMN(−) group than in the RG NMN(−) group, whereas that was significantly lower in the HG NMN(+) group than in the HG NMN(−) group. * p < 0.05; ** p < 0.0001