Table 1 RT-qPCR Primer Sequences
From: TGF-β3 regulates adhesion formation through the JNK/c-Jun pathway during flexor tendon healing
Gene | Forward(5′-3′) | Reverse(5′-3′) |
|---|---|---|
TGF-β3 | 5′-TGCGCCCCCTCTACATTG-3’ | 5′-GGTTCGTGGACCCATTTCC-3’ |
TGF-β1 | 5′-TGAGTGGCTGTCTTTTGACG-3’ | 5′-ACTTCCAACCCAGGTCCTTC-3’ |
TGF-β2 | 5′-ATGTGCAGGATAATTGCTGCC-3’ | 5′-TGGTGTTGTACAGGCTGAGG-3’ |
Smad3 | 5′-GCAGGCTCTCCAAACCTCT-3’ | 5′-GTGGAATGTCTCCCCAACTC-3’ |
Smad7 | 5′-CTCAAACCAACTGAGACTGTC-3’ | 5′-AGGCTCCAGAAGAAGTTGGG-3’ |
Mmp9 | 5′-GCCGTCTACTC CTCCCCGTG T-3’ | 5′-GTCTCTCTCCTACCCTCTGG-3’ |
Mmp2 | 5′-TCAGTCGATCACTAGCGTCAAT-3’ | 5′-CTAACTTCTCCCCACAGGGA-3’ |
β-actin | 5′-CACGATGGAGGGGCCGGACTCATC-3’ | 5′-TAAAGACCTCTATGCCAACACAGT-3’ |