Fig. 4
From: Influence of Angptl1 on osteoclast formation and osteoblastic phenotype in mouse cells
Effects of Angptl1 on osteoblast phenotype. a Total RNA was extracted from subconfluent MC3T3-E1 cells transiently transfected with empty vector or Angptl1. Then, qRT-PCR for Runx2, Osterix, ALP, osteocalcin, Angptl1 or GAPDH was performed. Data are expressed relative to the GAPDH mRNA value. Data represent mean ± SEM (n = 8 in each group). ##p < 0.01 by Mann-Whitney U test. b Mouse osteoblasts were cultured in the presence and absence of recombinant Angptl1 (100 ng/ml) for 24 h, and total RNA was extracted. Then, qRT-PCR for Runx2, Osterix, ALP, osteocalcin or GAPDH was performed. Data are expressed relative to the GAPDH mRNA value. Data represent mean ± SEM (n = 6 in each group). Cont; Control. (C) Mouse osteoblasts were cultured in the presence and absence of recombinant Angptl1 (100 ng/ml) for 24 h, and total RNA was extracted. Then, qRT-PCR for RANKL, OPG or GAPDH was performed. Data are expressed relative to the GAPDH mRNA value. Data represent mean ± SEM (n = 6 in each group). Cont; Control