Table 2 Two-way ANOVA results for protein and immuno/histochemical assays
Outcome | Tissue | Treatment Region (UE vs LE) | Group (Task vs C) | Treatment Region x Group Interaction |
|---|---|---|---|---|
CD68+ cells/mm2 | Median Nerve | F(1,26) = 5.78, p = 0.02 | F(1,26) = 5.74; p = 0.02 | F(1,26) = 5.62; p = 0.03 |
“ | Flexor digitorum muscle | F(1, 26) = 17.87, p = 0.0003 | F(1, 26) = 35.85; p < 0.0001 | F(1, 26) = 15.04; p = 0.0006 |
“ | Flexor digitorum tendon | F(1, 26) = 17.36, p = 0.0003 | F(1, 26) = 17.36; p = 0.0003 | F(1, 26) = 15.39; p = 0.0006 |
CD206+ (Mannose Receptor+) cells/mm2 | Median Nerve | F(1,26) = 3.96, p = 0.057 | F(1,26) = 9.16, p = 0.005 | F(1,26) = 2.87, p = 0.10 |
“ | Flexor digitorum muscle | F(1, 26) = 6.50; p = 0.01 | F(1, 26) = 36.14; p < 0.0001 | F(1, 26) = 4.75; p = 0.04 |
“ | Flexor digitorum tendon | F(1, 26) = 12.91; p = 0.001 | F(1, 26) = 19.55; p = 0.0002 | F(1, 26) = 14.66; p = 0.0007 |
Inflammatory Cytokines (pg/μg total protein) | ||||
IL-1β | Flexor digitorum muscle | F(1, 22) = 3.82, p = 0.06 | F(1, 22) = 7.49, p = 0.01 | F(1, 22) = 3.39, p = 0.08 |
IL-10 | “ | F(1, 21) = 8.07, p = 0.009 | F(1, 21) = 11.48, p = 0.003 | F(1, 21) = 8.32 p = 0.009 |
IL-18 | “ | F(1, 22) = 0.35, p = 0.56 | F(1, 22) = 0.38, p = 0.54 | F(1, 22) = 0.02, p = 0.89 |
CCL2/MCP-1 | “ | F(1, 22) = 0.006, p = 0.94 | F(1, 22) = 7.19, p = 0.94 | F(1, 22) = 0.66, p = 0.43 |
TNF-α | Serum | F(1, 26) = 0.30, p = 0.59 | F(1, 26) = 0.73, p = 0.40 | F(1, 26) = 01.22, p = 0.28 |
Extraneural Collagen % collagen staining | Median Nerve | F(1, 26) = 13.97; p = 0.0009 | F(1, 26) = 37.16; p < 0.0001 | F(1, 26) = 12.47; p = 0.002 |
Collagen Type I | ||||
% immunostaining | Flexor digitorum muscle | F(1, 26) = 17.02, p = 0.0003 | F(1, 26) = 45.53, p < 0.0001 | F(1, 26) = 18.05, p = 0.0002 |
pg/μg total protein | “ | F(1, 26) = 5.93, p = 0.024 | F(1, 26) = 4.34, p = 0.047 | F(1, 26) = 4.96; p = 0.04 |
Transforming Growth Factor beta 1 (TGF-β1) pg/μg total protein | Flexor digitorum muscle | F(1,26) = 0.10, p = 0.75 | F(1,26) = 0.22.74, p < 0.0001 | F(1,26) = 0.15, p = 0.70 |
Mid/Distal Flexor Digitorum Tendon Pathology Scores | ||||
Endotendon cell shape | Mid/Distal flexor digitorum tendon | F(1,26) = 3.94, p = 0.06 | F(1,26) = 47.21, p < 0.0001 | F(1,26) = 7.18, p = 0.01 |
Endotendon cellularity | “ | F(1,26) = 9.90, p = 0.004 | F(1,26) = 22.10, p < 0.0001 | F(1,26) = 4.18, p = 0.05 |
Endotendon collagen organization | “ | F(1,26) = 3.93, p = 0.049 | F(1,26) = 3.50, p = 0.07 | F(1,26) = 0.52, p = 0.48 |
Endotendon immune cells | “ | F(1,26) = 0.84, p = 0.37 | F(1,26) = 2.80, p = 0.11 | F(1,26) = 0.84, p = 0.37 |
Epitendon thickness | “ | F(1,26) = 4.11, p < 0.0001 | F(1,26) = 43.22, p = 0.05 | F(1,26) = 1.16, p = 0.29 |
Epitendon cellularity | “ | F(1,26) = 16.54, p = 0.09 | F(1,26) = 3.08, p = 0.0004 | F(1,26) = 2.15, p = 0.15 |
Epitendon immune cells | “ | F(1,26) = 4.61, p = 0.04 | F(1,26) = 18.14, p = 0.0002 | F(1,26) = 3.14, p = 0.09 |