Skip to main content

Table 2 Two-way ANOVA results for protein and immuno/histochemical assays

From: Key indicators of repetitive overuse-induced neuromuscular inflammation and fibrosis are prevented by manual therapy in a rat model

Outcome Tissue Treatment Region (UE vs LE) Group (Task vs C) Treatment Region x Group Interaction
CD68+ cells/mm2 Median Nerve F(1,26) = 5.78, p = 0.02 F(1,26) = 5.74; p = 0.02 F(1,26) = 5.62; p = 0.03
Flexor digitorum muscle F(1, 26) = 17.87, p = 0.0003 F(1, 26) = 35.85; p < 0.0001 F(1, 26) = 15.04; p = 0.0006
Flexor digitorum tendon F(1, 26) = 17.36, p = 0.0003 F(1, 26) = 17.36; p = 0.0003 F(1, 26) = 15.39; p = 0.0006
CD206+ (Mannose Receptor+) cells/mm2 Median Nerve F(1,26) = 3.96, p = 0.057 F(1,26) = 9.16, p = 0.005 F(1,26) = 2.87, p = 0.10
Flexor digitorum muscle F(1, 26) = 6.50; p = 0.01 F(1, 26) = 36.14; p < 0.0001 F(1, 26) = 4.75; p = 0.04
Flexor digitorum tendon F(1, 26) = 12.91; p = 0.001 F(1, 26) = 19.55; p = 0.0002 F(1, 26) = 14.66; p = 0.0007
Inflammatory Cytokines (pg/μg total protein)
 IL-1β Flexor digitorum muscle F(1, 22) = 3.82, p = 0.06 F(1, 22) = 7.49, p = 0.01 F(1, 22) = 3.39, p = 0.08
 IL-10 F(1, 21) = 8.07, p = 0.009 F(1, 21) = 11.48, p = 0.003 F(1, 21) = 8.32 p = 0.009
 IL-18 F(1, 22) = 0.35, p = 0.56 F(1, 22) = 0.38, p = 0.54 F(1, 22) = 0.02, p = 0.89
 CCL2/MCP-1 F(1, 22) = 0.006, p = 0.94 F(1, 22) = 7.19, p = 0.94 F(1, 22) = 0.66, p = 0.43
 TNF-α Serum F(1, 26) = 0.30, p = 0.59 F(1, 26) = 0.73, p = 0.40 F(1, 26) = 01.22, p = 0.28
Extraneural Collagen
% collagen staining
Median Nerve F(1, 26) = 13.97; p = 0.0009 F(1, 26) = 37.16; p < 0.0001 F(1, 26) = 12.47; p = 0.002
Collagen Type I
 % immunostaining Flexor digitorum muscle F(1, 26) = 17.02, p = 0.0003 F(1, 26) = 45.53, p < 0.0001 F(1, 26) = 18.05, p = 0.0002
 pg/μg total protein F(1, 26) = 5.93, p = 0.024 F(1, 26) = 4.34, p = 0.047 F(1, 26) = 4.96; p = 0.04
Transforming Growth Factor beta 1 (TGF-β1) pg/μg total protein Flexor digitorum muscle F(1,26) = 0.10, p = 0.75 F(1,26) = 0.22.74, p < 0.0001 F(1,26) = 0.15, p = 0.70
Mid/Distal Flexor Digitorum Tendon Pathology Scores
 Endotendon cell shape Mid/Distal flexor digitorum tendon F(1,26) = 3.94, p = 0.06 F(1,26) = 47.21, p < 0.0001 F(1,26) = 7.18, p = 0.01
 Endotendon cellularity F(1,26) = 9.90, p = 0.004 F(1,26) = 22.10, p < 0.0001 F(1,26) = 4.18, p = 0.05
 Endotendon collagen organization F(1,26) = 3.93, p = 0.049 F(1,26) = 3.50, p = 0.07 F(1,26) = 0.52, p = 0.48
 Endotendon immune cells F(1,26) = 0.84, p = 0.37 F(1,26) = 2.80, p = 0.11 F(1,26) = 0.84, p = 0.37
 Epitendon thickness F(1,26) = 4.11, p < 0.0001 F(1,26) = 43.22, p = 0.05 F(1,26) = 1.16, p = 0.29
 Epitendon cellularity F(1,26) = 16.54, p = 0.09 F(1,26) = 3.08, p = 0.0004 F(1,26) = 2.15, p = 0.15
 Epitendon immune cells F(1,26) = 4.61, p = 0.04 F(1,26) = 18.14, p = 0.0002 F(1,26) = 3.14, p = 0.09