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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis

Fig. 3

A. Showing the PCR amplification band of the positively cloned CBFB/pCDH plasmid vector was 564 bp, which was consistent with the sequence map. B. CBFB/ plkko.1 plasmid vector positive clones were 5 kbp and 2 kbp after double enzymatic digestion, and the sequencing results were consistent. C. Validating Western immunoblot results of plasmid transfection efficiency; D. cell proliferation in each group as detected by the CCK-8 assay; E, F. cellular apoptosis in each group as detected by Annexin V/PI double staining (*: p < 0.05, **: p < 0.01, ***: p < 0.001)

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