Table 1 The application of CRISPR/Cas9 in osteosarcoma research

CD11K

KHOS,U2OS

knock out

Proliferation↓, Migration↓,

Invasion↓, cell death↑

[37]

GLT25D1,

GLT25D2

Saos2

knock out

Non-survival cells

[38]

CD44

MNNG/HOS,

143B

knock out

Migration↓, Invasion↓, spheroids formation↓

[39]

CD81

143B

knock out

Tumors growth in mice↓

lung metastases↓

[40]

FGF5

MG63,U20S

knock out

Proliferation↓, tumor growth in mice↓, MAPK pathway activity↓

[41]

mutant TP53

KHOS,KHOSR2

knock out

Proliferation↓,migration↓,

clony formation↓,

IGF1-R↓,Bcl2↓,Survivin↓,

doxorubicin sensitivity↑

[42]

PD-L1

MNNG/HOS,

KHOS

knock out

Chemoresistance to doxorubicin and paclitaxel↓

[43]

CD44

KHOSR2,

U-2OSR2

knock out

Migration↓,Invasion↓,

spheroids formation↓,

doxorubicin sensitivity↑

[44]

ABCB1

KHOSR2,

U-2OSR

knock out

doxorubicin sensitivity↑

[45]

CNE9,

CNE10

U2OS

knock out

Proliferation↑,apoptosis↓,

SHOX expression↓

[46]

STAG2

U2OS

knock out

Proliferation↓,EMT↑,migration,cisplatin chemoresistance↑,PDL1,CDK4,RB expression↑,CCNB1,CCND1,CDK1↓,G2/M arrest,

PI3k/AKT Pathwayactivity↓

[47]

ESR1

143B

knock out

Proliferation↑,osteoblast differentiation↓,tumor growth and metastasis in mouse↑ VIMENTIN,SLUG,ZEB1,MMP9,SOX2,OCT4,NANOG expression↑

[48]

SENP2

HOS

knock out

Proliferation↑,migration↑,invasion↑,SOX9 expression↑

[49]

RECQL5

MG-63

knockin

Proliferation↓,apoptosis↑,cell cycle arrest,bcl-2↓,caspase-3↑

[50]

TP53

porcine zygotes

knock out

Genetical porcine model with mandibular osteosarcoma

[51]

RAD52

U2OS

knock out

Tumor Growth↓,lifespan↑,

DNA replication↓

[52]

PAWS1,

CD2AP

U2OS

knock out

knockin

Migration↓,Focal adhesions↓,cell adhesion ability↓

[53]

NRF2

U2OS

knock out

No function research

[54]

Cnn3

U2OS

knock out

Stress fiber networks organizations and contractility abnormalities.

stress fiber breakage events↑

[55]

G3BP

U2OS

knock out

For studying potential proviral roles of G3BP

[56]

CRY1,CRY2

U2OS

knock out

For studying circadian rhythms in mouse

[57]

SRGAP2

K12

knock out

Migration↑

[58]

VEGF

K7M2

knock out

Proliferation↓, Migration↓,

Invasion↓ in vivo and vitro.

[59]