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Table 1 The application of CRISPR/Cas9 in osteosarcoma research

From: Applications of CRISPR/Cas9 in the research of malignant musculoskeletal tumors

Target Genes Cell lines CRISPR/Cas9 Applications Effects References
CD11K KHOS,U2OS knock out Proliferation↓, Migration↓,
Invasion↓, cell death↑
[37]
GLT25D1,
GLT25D2
Saos2 knock out Non-survival cells [38]
CD44 MNNG/HOS,
143B
knock out Migration↓, Invasion↓, spheroids formation↓ [39]
CD81 143B knock out Tumors growth in mice↓
lung metastases↓
[40]
FGF5 MG63,U20S knock out Proliferation↓, tumor growth in mice↓, MAPK pathway activity↓ [41]
mutant TP53 KHOS,KHOSR2 knock out Proliferation↓,migration↓,
clony formation↓,
IGF1-R↓,Bcl2↓,Survivin↓,
doxorubicin sensitivity↑
[42]
PD-L1 MNNG/HOS,
KHOS
knock out Chemoresistance to doxorubicin and paclitaxel↓ [43]
CD44 KHOSR2,
U-2OSR2
knock out Migration↓,Invasion↓,
spheroids formation↓,
doxorubicin sensitivity↑
[44]
ABCB1 KHOSR2,
U-2OSR
knock out doxorubicin sensitivity↑ [45]
CNE9,
CNE10
U2OS knock out Proliferation↑,apoptosis↓,
SHOX expression↓
[46]
STAG2 U2OS knock out Proliferation↓,EMT↑,migration,cisplatin chemoresistance↑,PDL1,CDK4,RB expression↑,CCNB1,CCND1,CDK1↓,G2/M arrest,
PI3k/AKT Pathwayactivity↓
[47]
ESR1 143B knock out Proliferation↑,osteoblast differentiation↓,tumor growth and metastasis in mouse↑ VIMENTIN,SLUG,ZEB1,MMP9,SOX2,OCT4,NANOG expression↑ [48]
SENP2 HOS knock out Proliferation↑,migration↑,invasion↑,SOX9 expression↑ [49]
RECQL5 MG-63 knockin Proliferation↓,apoptosis↑,cell cycle arrest,bcl-2↓,caspase-3↑ [50]
TP53 porcine zygotes knock out Genetical porcine model with mandibular osteosarcoma [51]
RAD52 U2OS knock out Tumor Growth↓,lifespan↑,
DNA replication↓
[52]
PAWS1,
CD2AP
U2OS knock out
knockin
Migration↓,Focal adhesions↓,cell adhesion ability↓ [53]
NRF2 U2OS knock out No function research [54]
Cnn3 U2OS knock out Stress fiber networks organizations and contractility abnormalities.
stress fiber breakage events↑
[55]
G3BP U2OS knock out For studying potential proviral roles of G3BP [56]
CRY1,CRY2 U2OS knock out For studying circadian rhythms in mouse [57]
SRGAP2 K12 knock out Migration↑ [58]
VEGF K7M2 knock out Proliferation↓, Migration↓,
Invasion↓ in vivo and vitro.
[59]
  1. Notes: ↑:Up-regulation: ↓:Down-regulation