Fig. 6
Biochemical composition of MSCs during three weeks of pellet culture following adenoviral gene transfer with GFP, SOX9, TGFB1 or IGF1 alone or in combination. Primary MSCs were infected with Ad.GFP (Control), Ad.SOX9 (SOX9), Ad.TGFB1 + Ad.SOX9 (SOX9 + TGFB1), Ad.TGFB1 alone (TGFB1), Ad.IGF1 + Ad.SOX9 (SOX9 + IGF1), or Ad.IGF1 alone (IGF1) at 5 × 102 vp/cell, seeded into aggregate cultures and maintained in serum-free medium for 21 days. At days 3, 7, 14 and 21 biochemical assays were performed to measure (a) cell proliferation by the ATP assay, (b) DNA content, (c) GAG synthesis via GAG/DNA ratios, and (d) ALP activity normalized to the DNA content. (a-d) The data represent mean values ± SD from n = 3 aggregates per condition and time point from marrow preparations of n = 3 different patients. Asterisks indicate values that are statistically different (p < 0.05) from marker gene vector–transduced control cultures or between samples