Skip to main content
Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Polyphyllin VII attenuated RANKL-induced osteoclast differentiation via inhibiting of TRAF6/c-Src/PI3K pathway and ROS production

Fig. 4

Effect of polyphyllin VII (PP7) on gene expression of TRACP5, Ctsk, and NFATc1 in the differentiated osteoclasts. After pretreatment with various concentrations of polyphyllin VII, BMMs were induced to differentiate into osteoclasts in the presence or absence of RANKL and M-CSF. After 24 h treatment, mRNA levels of TRACP5, Ctsk, and NFATc1 were quantified using RT-PCR as described in the methods. Panel a TRACP5 mRNA expression. Vertical axis: TRACP5 mRNA expression; horizontal axis: polyphyllin VII (PP7) concentrations (μM). Panel b Ctsk mRNA expression. Vertical axis: Ctsk mRNA expression; horizontal axis: polyphyllin VII (PP7) concentrations (μM). Panel c NFATc1 mRNA expression. Vertical axis: NFATc1 mRNA expression; horizontal axis: polyphyllin VII (PP7) concentrations (μM). * P < 0.05 as compared to the cells treated with 0 μM of polyphyllin VII. Blank control: BMM cells without RANKL or PP7 treatment

Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us