Fig. 3

Reduced M1 macrophage numbers suppress M1 macrophage infiltration and muscle/bone atrophy after nerve injury. We used a chronic constriction injury (CCI) model mouse model as a nerve injury model. a Osteoclast numbers were lower as per immunohistochemical analysis of the femoral bone in the macrophage depletion group 1 week postoperatively than in the untreated group (n = 8, *P < 0.05). Center lines of box plot indicate median, box limits indicate the 25th and 75th percentiles, and whiskers extend to minimum and maximum values. b M1 macrophage numbers were lower as per flow cytometry of the muscles in the macrophage depletion group 1 week postoperatively than in the untreated group (n = 8, *P < 0.05). c Hindlimb muscle weight was higher in the macrophage depletion group1 week postoperatively than in the untreated group (n = 8, *P < 0.05). d In the macrophage depletion group, there was no significance of total femoral and tibial bone densities in 2 weeks after CCI (n = 8). Dot plots indicate median, and error bars indicate the 25th and 75th percentiles