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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Higher aggrecan 1-F21 epitope concentration in synovial fluid early after anterior cruciate ligament injury is associated with worse knee cartilage quality assessed by gadolinium enhanced magnetic resonance imaging 20 years later

Fig. 3

Western blot of synovial fluid and cartilage samples. a Synovial fluid A1 and D1 samples on membranes probed with antibodies against 6-sulfated chondroitin sulfate stubs (3B3), aggrecan epitope 1-F21 and ARGS-aggrecan. b ADAMTS-4 or MMP-3 in vitro digested cartilage A1D1 aggrecan samples on membranes probed with antibodies against aggrecan epitope 1-F21 and G1-domain of aggrecan. The position of Mw markers (left side) and the immunobands are indicated. The images are from different experiments showing representative signals. The original images from full size blotted gels are shown in Fig. S2. Keratan sulfate region (KS), chondroitin sulfate region (CS) and globular domains (G1, G2 and G3) are illustrated in Fig. 4. One to three μg sGAG was loaded per well. IGD = interglobular domain

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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