Fig. 3

RCI treatment reduced the number of ankle monocytes/macrophages and osteoclasts in rats with CIA. Quantitative analysis scoring of (a) CD68–positive monocytes/macrophages and (b) cathepsin K–positive osteoclasts were determined using Visiopharm quantitative digital pathology software. Values are represented as mean number of positive stained cells per area ± SEM. ^a p ≤ 0.05 vs disease control. Statistics were analyzed by 1-way ANOVA followed by the Dunnett’s multiple comparisons test. Abbreviations: ANOVA, analysis of variance; CIA, collagen-induced arthritis; ETN, etanercept; RCI, repository corticotropin injection; SEM, standard error of the mean