Fig. 3

MiR-410-3p targets HMGB1 in mouse primary chondrocytes. a, Two predicted binding sites of miR-410-3p in the 3′ UTR region of HMGB1. b, Relative dual-luciferase activity in mouse primary chondrocytes by cloning a fragment of the wild type (WT) or mutant (MT) 3′ UTR of HMGB1 for both binding sites into the luciferase reporter vector and followed by cotransfection with miR-410-3p mimics or NC mimics into 293 T cells. c, qRT-PCR detection of transcript levels of HMGB1 in control, LPS treated mouse primary chondrocyte (LPS + NC) and overexpression of miR-410-3p in LPS treated mouse primary chondrocyte (LPS + miR-410-3p mimics). d, Western blot analysis detecting protein expression of HMGB1 in control, LPS + NC and LPS + miR-410-3p mimics mouse primary chondrocytes. β-actin was used as a loading control. The value of the control was set at ‘1’ in qRT-PCR analysis. **P < 0.01 vs NC or Control; ##P < 0.01 vs LPS + NC