Fig. 4

Bilobalide reduced the activation of p38, ERK1/2, JNK, and NF-κB p65 in skeletal muscle following I/R. a Representative immunoblots of P-P38, total P38, P-ERK1/2, total ERK1/2, P-JNK, total JNK, P65 and β-actin in skeletal muscle. The total amount of protein loaded per lane was 40 μg. Detection of β-actin on the same blots was used to verify equal loading among the various lanes. b-e Bar graph illustrating the average relative expression of P-P38, total P38 (b), P-ERK1/2, total ERK1/2 (c), P-JNK, total JNK (d) and P65 (e) in each group. The protein levels were quantified by camera-based detection of emitted chemiluminescence. ^*P < 0.05 and ^**p < 0.01 compared with the Sham group; ^#p < 0.05 and ^##p < 0.01 compared with the I/R group; ns = not significant compared with the I/R + bilobalide-low group. n = 8 per group