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Table 1 Detailed description of the four different donor tissues used for the isolation of BM-MSCs and MSC-like cells

From: The human arthritic hip joint is a source of mesenchymal stromal cells (MSCs) with extensive multipotent differentiation potential

 

Donor tissue

Surgical extraction

BM-MSCs

Bone marrow of the proximal femur

In order to fix the artificial shaft of the hip prothesis in the proximal femur, reaming of the proximal femoral shaft is necessary. The bone marrow cylinder removed during this procedure was secured.

MSC-like cells

Arthritic hyaline cartilage of the femoral head

The femoral head is removed during total hip arthroplasty. Macroscopically eroded, hyaline cartilage was scraped off the femoral head using a scalpel.

Full-thickness samples of the anterior joint capsule

The hip joint was accessed through the anterior joint capsule. The full-thickness sample of the joint capsule removed during this procedure was secured.

Ligamentum capitis femoris (LCF)

The LCF was attached to the femoral head which is removed during total hip arthroplasty. The LCF was removed from the femoral head using a scalpel.

  1. Precise description of the four different donor tissues that were harvested during hip replacement surgery and were later used for the isolation of BM-MSCs and MSC-like cells from hyaline cartilage, the LCF and the hip joint’s anterior capsule