Fig. 3

FOXO1 was the target of miR-532-5p. a Putative target sequence of FOXO1 on the 3′-UTR of miR-532-5p.(b) Protein expression level of FOXO1 in C2C12 cells. c Expression levels of FOXO1 in C2C12 cells. d Luciferase activity assay after co-transfection of C2C12 cells with a miR-532-5p mimetic and a reporter plasmid containing wild type (WT) or Mut (MT) 3’-UTR of FOXO1. ** P < 0.01